Neutrophils infiltrate systemic vasculature of women with preeclampsia, so we tested the hypothesis that factors in plasma of preeclamptic women activate endothelial cells to produce IL-8 resulting in transendothelial migration of neutrophils. Neutrophil migration was studied using the Transwell system. An endothelial cell line was grown to confluence on the inserts and treated with 10% plasma from normal nonpregnant (NNP), normal pregnant (NP) and preeclamptic (PE) women or with an oxidizing solution containing linoleic acid (OxLA). Compared to medium control, NNP plasma or NP plasma, PE plasma significantly stimulated IL-8 and neutrophil migration which was inhibited by vitamins E and C or IL-8 neutralizing antibody. Compared to medium control or LA, OxLA stimulated IL-8 and neutrophil migration which was inhibited by vitamins E and C or IL-8 antibody. Conclusion: Factors present in plasma of preeclamptic women stimulate transendothelial migration of neutrophils which is due to induction of oxidative stress and production of IL-8.
Gervasi MT, Chaiworapongsa T., Pacora P., et al. Phenotypic and metabolic characteristics of monocytes and granulocytes in preeclampsia. Am J Obstet Gynecol. 2001 ;185:792-797.
Haeger M., Unander M., Norder-Hansson B., Tylman M., Bengtsson A.Complement, neutrophil, and macrophage activation in women with severe preeclampsia and the syndrome of hemolysis, elevated liver enzymes, and low platelet count . Obstet Gynecol. 1992;79:19-26.
4.
Sacks GP, Studena K., Sargent K., Redman CWNormal pregnancy and preeclampsia both produce inflammatory changes in peripheral blood leukocytes akin to those of sepsis. Am J Obstet Gynecol . 1998;179:80-86.
5.
Tsukimori K. , Maeda H., Ishida K., Nagata H., Koyanagi T., Nakano H.The superoxide generation of neutrophils in normal and preeclamptic pregnancies. Obstet Gynecol. 1993;81:536-540.
6.
Gorog P.Activation of human blood monocytes by oxidized polyunsaturated fatty acids: a possible mechanism for the generation of lipid peroxides in the circulation . Int J Exp Path. 1991;72:227-237.
7.
Vaughan JE, Walsh SWNeutrophils from pregnant women produce thromboxane and tumor necrosis factor-alpha in response to linoleic acid and oxidative stress. Am J Obstet Gynecol. 2005;193:830-835.
8.
Vaughan JE, Walsh SW, Ford GDThromboxane mediates neutrophil superoxide production in pregnancy. Am J Obstet Gynecol. 2006;195:1415-1420.
9.
Walsh SW, Vaughan JE, Wang Y., Roberts LJ II.Placental isoprostane is significantly increased in preeclampsia. FASEB J. 2000;14:1289-1296.
10.
Walsh SW, Wang Y.Secretion of lipid peroxides by the human placenta. Am J Obstet Gynecol. 1993;169:1462-1466.
11.
Walsh SW, Wang Y.Trophoblast and placental villous core production of lipid peroxides, thromboxane, and prostacyclin in preeclampsia. J Clin Endocrinol Metab . 1995;80:1888-1893.
12.
Mellembakken JR, Aukrust P., Olafsen MK, Ueland T., Hestdal K., Videm V.Activation of leukocytes during the uteroplacental passage in preeclampsia. Hypertension. 2002 ;39:155-160.
13.
Walsh SWLipid peroxidation in pregnancy. Hypertens Pregnancy. 1994;13:1-32.
14.
Leik CE, Walsh SWNeutrophils infiltrate resistance-sized vessels of subcutaneous fat in women with preeclampsia. Hypertension. 2004;44:72-77.
15.
Shah TJ, Walsh SWActivation of NF-kappaB and expression of COX-2 in association with neutrophil infiltration in systemic vascular tissue of women with preeclampsia. Am J Obstet Gynecol. 2007;196:48.e41-48.e48.
16.
Smart SJ, Casale TBInterleukin-8-induced transcellular neutrophil migration is facilitated by endothelial and pulmonary epithelial cells. Am J Respir Cell Mol Biol. 1993;9:489-495.
17.
Smart SJ, Casale TBTNF-alpha-induced transendothelial neutrophil migration is IL-8 dependent . Am J Physiol. 1994;266:L238-L245.
18.
Wiedermann CJ, Schratzberger P., Kahler CMMigration of neutrophils across endothelial monolayers is stimulated by treatment of the monolayers with beta-endorphin . Brain Behav Immun. 1994;8:270-277.
19.
Leik CE, Walsh SWLinoleic acid, but not oleic acid, upregulates production of interleukin-8 by human vascular smooth muscle cells via arachidonic acid metabolites under conditions of oxidative stress. J Soc Gynecol Investig. 2005;12:593-598.
20.
Jentzsch AM , Bachmann H., Furst P., Biesalski HKImproved analysis of malondialdehyde in human body fluids. Free Radic Biol Med. 1996;20:251-256.
21.
Roberts JM, Taylor RN, Musci TJ, Rodgers GM, Hubel CA, McLaughlin MKPreeclampsia: an endothelial cell disorder. Am J Obstet Gynecol . 1989;161:1200-1204.
22.
Taylor RN, de Groot CJ, Cho YK, Lim KHCirculating factors as markers and mediators of endothelial cell dysfunction in preeclampsia. Semin Reprod Endocrinol. 1998;16:17-31.
23.
Endresen MJ , Morris JM, Nobrega AC, Buckley D., Linton EA, Redman CWSerum from preeclamptic women induces vascular cell adhesion molecule-1 expression on human endothelial cells in vitro: a possible role of increased circulating levels of free fatty acids. Am J Obstet Gynecol. 1998;179:665-670.
24.
Takacs P., Kauma SW, Sholley MM, Walsh SW, Dinsmoor MJ, Green K.Increased circulating lipid peroxides in severe preeclampsia activate NF-kappaB and upregulate ICAM-1 in vascular endothelial cells. FASEB J. 2001;15: 279-281.
25.
Lorentzen B. , Henriksen T.Plasma lipids and vascular dysfunction in preeclampsia. Semin Reprod Endocrinol. 1998 ;16:33-39.
26.
Hubel CAOxidative stress in the pathogenesis of preeclampsia. Proc Soc Exp Biol Med. 1999;222:222-235.
27.
Walsh SWMaternal-placental interactions of oxidative stress and antioxidants in preeclampsia . Semin Reprod Endocrinol. 1998;16:93-104.
28.
Veenstra van Nieuwenhoven AL, Bouman A., Moes H., et al. Cytokine production in natural killer cells and lymphocytes in pregnant women compared with women in the follicular phase of the ovarian cycle. Fertil Steril. 2002;77: 1032-1037.
29.
Lurie S., Frenkel E., Tuvbin Y.Comparison of the differential distribution of leukocytes in preeclampsia versus uncomplicated pregnancy. Gynecol Obstet Invest. 1998;45:229-231.
30.
Meziani F., Tesse A., David E., et al. Shed membrane particles from preeclamptic women generate vascular wall inflammation and blunt vascular contractility. Am J Pathol . 2006;169: 1473-1483.
Branch DW, Mitchell MD, Miller E., Palinski W., Witztum JLPre-eclampsia and serum antibodies to oxidised low-density lipoprotein. Lancet. 1994;343:645-646.
33.
Diedrich F. , Renner A., Rath W., Kuhn W., Wieland E.Lipid hydroperoxides and free radical scavenging enzyme activities in preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelet count) syndrome: no evidence for circulating primary products of lipid peroxidation. Am J Obstet Gynecol. 2001;185:166-172.
34.
Gratacos E. , Casals E., Deulofeu R., et al. Serum antibodies to oxidized low-density lipoprotein in pregnant women with preeclampsia and chronic hypertension: lack of correlation with lipid peroxides. Hypertens Pregnancy . 2001;20: 177-183.
