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Abstract

The dynamics of the subcellular distribution of PLCβ1 was investigated during meiosis competence acquisition and meiosis resumption in relation to oocyte diameter and to nonsurrounded-nucleolus or surrounded-nucleolus chromatin configurations. Oocytes collected after both in vivo and in vitro folliculogenesis were studied. In both conditions, at the beginning of the process, most oocytes exhibited a nuclear PLCβ 1 associated with a nonsurrounded-nucleolus chromatin configuration. Then at the final stage of the process, the factors shifted mainly toward a cytoplasmic PLCβ1 and a surrounded-nucleolus chromatin configuration, typical of a preovulatory fertilizable oocyte. Additionally, only germinal vesicle oocytes with a diameter > 75 μm, and exhibiting cytoplasmic PLC β1 distribution and surrounded-nucleolus chromatin configuration, resumed meiosis. Our findings demonstrate a strong correlation between oocyte diameter, chromatin configuration, and PLCβ1 localization. Thus, PLCβ1 localization appears to be a key factor determining the progressive acquisition of meiotic competence and final resumption of meiosis.

Keywords

Oocyte PLCβ1 meiosis folliculogenesis chromatin growth mouse.

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Poirot C. , Vacher-Lavenu MC , Helardot P. , Guibert J. , Brugières L. , Jouannet P. Human ovarian tissue cryopreservation: indications and feasibility . Hum Reprod. 2002;17:1447-1452.

Eppig JJ , Schroeder AC Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro. Biol Reprod. 1989;41:268-276.

Spears N. , Boland NI , Murray AA , Gosden RG Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile . Hum Reprod. 1994;9:527-532.

Cortvrindt R. , Smitz J. , Van Steirteghem AC In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system . Hum Reprod. 1996;11: 2656-2666.

Pesty A. , Miyara F. , Debey P. , Lefevre B. , Poirot C. Multiparameter assessment of mouse oogenesis during follicular growth in vitro . Mol Hum Reprod. 2007;13:3-9.

Gomes JE , Pesty A. , Gouveia-Oliveira A. , Cidadão AJ , Plancha CE , Lefèvre B. Age and gonadotropins control Ca2+-spike acquisition in mouse oocytes isolated from early preantral follicles. Int J Dev Biol. 1999;43:839-842.

Faenza I. , Bregoli L. , Ramazzotti G. , et al. Nuclear phospholipase C beta1 and cellular differentiation. Front Biosci. 2008;13:2452-2463.

Pesty A. , Avazeri N. , Lefèvre B. Nuclear calcium release by InsP3-receptor channels plays a role in meiosis reinitiation in the mouse oocyte. Cell Calcium. 1998;24:239-251.

Avazeri N. , Courtot A-M. , Pesty A. , Duquenne C. , Lefèvre B. Cytoplasmic nuclear phospholipase C-beta 1 relocation: role in resumption of meiosis in the mouse oocyte. Mol Biol Cell. 2000;11:4369-4380.

10.

Avazeri N. , Courtot A-M. , Pesty A. , Lefevre B. Meiosis resumption, calcium-sensitive period, and PLC-β1 relocation into the nucleus in the mouse oocyte. Cell Signal. 2003;15: 1003-1010.

11.

Bouniol-Baly C. , Hamraoui L. , Guibert J. , Beaujean N. , Szöllösi MS , Debey P. Differential transcriptional activity associated with chromatin configuration in fully-grown mouse germinal vesicle oocytes . Biol Reprod. 1999;60:580-587.

12.

Christians E. , Boiani M. , Garagna S. , et al. Gene expression and chromatin organization during mouse oocyte growth. Dev Biol. 1999 ;207:76-85.

13.

York JD , Majerus PW Nuclear phosphatidylinositols decrease during S-phase of the cell cycle in HeLa cells. J Biol Chem. 1994;269:7847-7850.

14.

Manzoli L. , Billi AM , Gilmour RS , et al. Phosphoinositide signaling in nuclei of Friend cells: tiazofurin down-regulates phospholipase C beta 1. Cancer Res. 1995;55:2978-2980.

15.

Manzoli L. , Billi AM , Rubbini S. , et al. Essential role for nuclear phospholipase C beta1 in insulin-like growth factor I-induced mitogenesis. Cancer Res. 1997;57:2137-2139.

16.

Faenza I. , Ramazzotti G. , Bavelloni A. , et al. Inositidedependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter. Endocrinology. 2007;148:1108-1117.

17.

Baker SJ , Srsen V. , Lapping R. , Spears N. Combined effect of follicle-follicle interactions and declining follicle-stimulating hormone on murine follicle health in vitro. Biol Reprod. 2001;65:1304-1310.

18.

Spears N. , Baker S. , Srsen V. , et al. Mouse ovarian follicles secrete factors affecting the growth and development of like-sized ovarian follicles in vitro. Biol Reprod. 2002;67:1726-1733.

19.

Eppig JJ , Schultz RM , O'Brien M. , Chesnel F. Relationships between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Dev Biol. 1994 ;164: 1-9.

20.

Lefèvre B. , Pesty A. , Testart J. Cytoplasmic and nucleic calcium oscillations in immature mouse oocytes: evidence of wave polarization by confocal imaging. Exp Cell Res. 1995;218:166-173.

21.

Gilchrist RB , Ritter LJ , Myllymaa S. , et al. Molecular basis of oocyte-paracrine signaling that promotes granulosa cell proliferation. J Cell Sci. 2006;119:3811-3821.

22.

Wang Q. , Sun OY Evaluation of oocyte quality: morphological, cellular and molecular predictors . Reprod Fertil Dev. 2007;19:1-12.

23.

Murray A. , Lannagan T. , Kind P. , et al. Investigation of the effect of PLC-β1 in mouse reproduction . The Society for the Study of Reproduction, 37th annual meeting, 2004. Abstract 235.

24.

Ballester M. , Molist J. , Lopez-Bejar M. , et al. Disruption of the mouse phospholipase C-β1 gene in a β-lactoglobulin transgenic line affects viability, growth, and fertility in mice. Gene. 2004;341:279-289.

25.

Reddy P. , Liu L. , Adhikari D. , et al. Oocyte-specific deletion of Pten causes premature activation of the primordial follicle pool. Science. 2008;319:611-613.

The Role of PLCβ1 in the Control of Oocyte Meiosis During Folliculogenesis

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