Bile is one matrix type that may be collected at autopsy and submitted to the toxicology laboratory for analysis. Because it is an excretion product of the liver, it can be used for screening purposes and to determine what drugs an individual used or was exposed to prior to death. This paper presents collection and analytical considerations of bile, and provides an overview of its utility from a testing and interpretation perspective. Acad Forensic Pathol. 2018 8(2): 324-327
RhoadesRATannerGA. Medical physiology. Boston: Little Brown; 1995. 839 p.
2.
BévalotFCartiserNBottinelliC. State of the art in bile analysis in forensic toxicology. Forensic Sci Int. 20162; 259:133–54. PMID: 26773224. https://doi.org/10.1016/j.forsciint.2015.10.034.
3.
KarchSB. Postmortem toxicology of abused drugs. Boca Raton: CRC Press; 2007. 212 p.
AgarwalALemosN. Significance of bile analysis in drug-induced deaths*. J Anal Toxicol. 19961-2; 20(1):61–3. PMID: 8837955. https://doi.org/10.1093/jat/20.1.61.
6.
LevineB. Principles of forensic toxicology. 4th ed. Washington: AACC Press; 2013. 550 p.
7.
YamaguchiKGodaTYamakiSOhnoY. Structural analysis of quazepam metabolites in bile by ion trap time-of-flight mass spectrometry. Forensic Sci Int. 2015 Nov; 256:7-16. PMID: 26301753. https://doi.org/10.1016/j.forsciint.2015.07.016.
8.
AmplatzBZöhrerEHaasC. Bile acid preparation and comprehensive analysis by high performance liquid chromatographyhigh-resolution mass spectrometry. Clin Chim Acta. 20171; 464: 85–92. PMID: 27838249. https://doi.org/10.1016/j.cca.2016.11.014.
9.
FernándezPAldonzaMBouzasA. GC-FID determination of cocaine and its metabolites in human bile and vitreous humor. J Appl Toxicol. 20065-6; 26(3):253–7. PMID: 16389661. https://doi.org/10.1002/jat.1130.
10.
RobertsonMDDrummerOH. Postmortem distribution and redistribution of nitrobenzodiazepines in man. J Forensic Sci. 19981; 43(1):9–13. PMID: 9456518. https://doi.org/10.1520/jfs16082j.
11.
FantonLBevalotFGustinMP. Interpretation of drug concentrations in an alternative matrix: the case of meprobamate in bile. Int J Legal Med. 20093; 123(2):97–102. PMID: 18581126. https://doi.org/10.1007/s00414-008-0259-x.
12.
TominagaMMichiueTOritaniS. Evaluation of postmortem drug concentrations in bile compared with blood and urine in forensic autopsy cases. J Anal Toxicol. 20166; 40(5):367–73. PMID: 27185819. https://doi.org/10.1093/jat/bkw028.
