Pollination and fertilization are critical determinants of fruit set and quality in raspberry. To optimize cross-breeding strategies and cultivation practices for raspberry, this study investigated key factors influencing pollination efficiency, including stigma receptivity and pollen tube growth dynamics. Results indicated that raspberry exhibited a wet stigma morphology with a solid-style structure containing a guiding tissue. Maximum stigma receptivity was observed at the petal opening stage. Pollen germination on the stigma initiated within 4 h post-pollination, with pollen tubes reaching the ovary base by 12 h, culminating in successful double fertilization. Embryo sac activity remained elevated throughout the first 48 h post-pollination, achieving an exceptional fruit set rate exceeding 80%.
ShahHMSSinghZHasanMU, et al.Preharvest methyl jasmonate application delays cell wall degradation and upregulates phenolic metabolism and antioxidant activities in cold stored raspberries. Food Chem2025; 462: 141020.
2.
ShakyaR. Stigma receptivity with pollen in sunflower accompanies novel histochemical and biochemical changes in both male and female reproductive structures. Vegetos2020; 33: 376–384.
3.
RiellaVBorgesAArias-SibillotteM, et al.The effective pollination period of the olive cultivar ‘Arbequina’(Olea europaea L.) in a non-traditional production region. S Afr J Bot2022; 151: 246–254.
4.
MitchellHKniestHWon-JinO. Digital photogrammetry and microscope photographs. Photogrammetric Record1999; 16: 695–704.
5.
HaoQXuLWangH, et al.Evaluation of pollen viability, stigma receptivity, and the cross barrier between tropical and hardy water lily cultivars. Flora2022; 290: 152046.
6.
SeverynsAClaessenHSwennenR, et al.Functional analysis of pollen tube growth dynamics in banana (Musa spp.). Sci Hortic2025; 349: 114268.
7.
LosadaJMHerreroM. Flower strategy and stigma performance in the apple inflorescence. Sci Hortic2013; 150: 283–289.
8.
Heslop-HarrisonYShivannaK. The receptive surface of the angiosperm stigma. Ann Bot1977; 41: 1233–1258.
9.
QinFShenYLiZ, et al.Shade delayed flowering phenology and decreased reproductive growth of Medicago sativa L.. Front Plant Sci2022; 13: 835380.
10.
ZhangHWangQWeiJ. Study on flowering dynamics and pollination habits of monochasma savatieri under artificial cultivation conditions. Plants2025; 14: 15.
11.
JianQHaoranJShoumeiZ, et al.Study on pollen viability and stigma acceptability of Guava. Chinese Agricultur Sci Bull2024; 40: 70–75.
12.
ZhaoyanCLongDHuqingW, et al.Pollen viability, stigma receptivity and their effect on fruit set of passionfruit at different flower developmental stages. J Fruit Sci2023; 40: 969–977.
13.
SteinacherGWagnerJ. Flower longevity and duration of pistil receptivity in high mountain plants. Flora-morphol, distrib, funct ecol plants2010; 205: 376–387.
14.
PanCChenZZhangM, et al.Effects of flowering period on floral traits, pollinator behavior and seed production of david’s mountain laurel (Sophora davidii). Plant Signal Behav2024; 19: 2383823.
15.
SunXWangFCuiR, et al.Studies on reproductive strategies of Vitex negundo L. Var.heterophylla(franch.) rehder (Lamiaceae) based on morphological characteristics and SSR markers. Ecol Evol2020; 10: 5270–5280.
16.
ZhangLZengTHuH, et al.Interspecific divergence of two sinalliaria (Brassicaceae) Species in eastern China. Front Plant Sci2018; 9: 77.
17.
WangCXieYMaL. Flower traits and breeding system of Rhododendron platypodum diels, an endangered plant species in China. PloS one2025; 20: e0319513.
18.
KarimiHRMohammadiNEstajiA, et al.Effect of supplementary pollination using enriched pollen suspension with zn on fruit set and fruit quality of pistachio. Sci Hortic2017; 216: 272–277.
19.
IgawaTYanagawaYMiyagishimaS-Y, et al.Analysis of gamete membrane dynamics during double fertilization of Arabidopsis. J Plant Res2013; 126: 387–394.
20.
ChenKWangQYuX, et al.OsSRF8 interacts with OsINP1 and OsDAF1 to regulate pollen aperture formation in rice. Nat Commun2024; 15: 4512.
21.
XiaoHRadovichCWeltyN, et al.Integration of tomato reproductive developmental landmarks and expression profiles, and the effect of SUN on fruit shape. BMC Plant Biol2009; 9: 49.
22.
AlcarazMLHormazaJIRodrigoJ. Pistil starch reserves at anthesis correlate with final flower fate in avocado (Persea americana). PLoS One2013; 8: e78467.
23.
RanePThakreMVermaMK, et al.Studies on pollen micro-morphology, pollen storage methods, and cross-compatibility among grape (Vitis spp.) genotypes. Front Plant Sci2024; 15: 1353808.
