Blackberry (Rubus subg. Rubus Watson) is valued for its nutritional content, and optimizing its production is crucial in temperate regions like Serbia. Plant growth regulators (PGRs), such as Regalis (Prohexadione-Ca), have potential to enhance yield and fruit quality by modulating plant growth. This study evaluates the effects of Regalis on the ‘Čačanska Bestrna’ blackberry cultivar in Serbia.
Objective
This study aims to assess the impact of Regalis on vegetative growth, yield, fruit quality, and nutrient composition of blackberries.
Methods
The study was conducted at two locations in Western Serbia on blackberry canes foliar treated with the growth regulator Regalis and compared to untreated control bushes. Physical and chemical fruit traits were determined by validated analytical procedures. Mineral content was analyzed by atomic absorption spectrophotometry.
Results
The results showed that Regalis significantly improved yield, fruit size, soluble solids, sugars, and micronutrient content, with effects varying by cultivation site.
Conclusions
Regalis is an effective tool for enhancing blackberry yield and quality, especially under specific agro-ecological conditions in Serbia. Further research on long-term effects is recommended.
PavlovićAVPapettiAZagoracDČD, et al.Phenolics composition of leaf extracts of raspberry and blackberry cultivars grown in Serbia. Ind Crop Prod2016; 87: 304–314.
4.
MiloševićTMiloševićNGlišićI,et al.Fruit quality attributes of blackberry grown under limited environmental conditions. Plant Soil Environ2012; 58: 322–327.
5.
StanisavljevićM. New small fruit cultivars from Čačak: 1. The new blackberry (Rubus sp.) cultivar ‘Čačanska Bestrna’. Acta Hortic1999; 505: 291–296.
6.
Statistical Office of the Republic of Serbia. Available from: http://www.stat.gov.rs (2024, accessed 15 December 2024).
7.
RamírezHLeza-HernándezPCBenavidesA, et al.Prohexadione-Ca modifies content of gibberellins and vitamin C, antioxidant capacity and enzymatic activity in apple. Acta Hortic2010; 884: 139–144.
8.
LiuHZhaoHLyuL, et al.Synergistic effect of natural antifungal agents for postharvest diseases of blackberry fruits. J Sci Food Agric2019; 99: 3343–3349.
9.
Martin-RivillaHGarcia-VillaracoARamos-SolanoB, et al.Metabolic elicitors of Pseudomonas fluorescens N 21.4 elicit flavonoid metabolism in blackberry fruit. J Sci Food Agric2021; 101: 205–214.
10.
GowdVBaoTWangL, et al.Antioxidant and antidiabetic activity of blackberry after gastrointestinal digestion and human gut microbiota fermentation. Food Chem2018; 269: 618–627.
11.
HusseinJEl-NaggarMEFoudaMMG, et al.The efficiency of blackberry loaded AgNPs, AuNPs and Ag@AuNPs mediated pectin in the treatment of cisplatin-induced cardiotoxicity in experimental rats. Int J Biol Macromol2020; 159: 1084–1093.
12.
SolarovSEljugaGIlićM, et al.Traditional use of medicinal plants in rural areas of Osijek-Baranja county, Republic of Croatia. Lekovite Sirovine2022; 42: 10–23.
13.
OliveiraDMKwiatkowskiARosaCI,et al.Refrigeration and edible coatings in blackberry (Rubus spp.) conservation. J Food Sci Techn2014; 51: 2120–2126.
14.
MedjdoubRValJBlancoA. Physiological effects of prohexadione-calcium in apple trees: effects on parameters related to photoproductivity. J Hortic Sci Biotech2007; 82: 126–132.
15.
RamírezHLeza-HernándezPCBenavidesA, et al.Prohexadione-Ca modifies content of gibberellins and vitamin C, antioxidant capacity and enzymatic activity in apple. Acta Hortic2010; 884: 139–144.
16.
SouthwickSMIngelsCHansenR,et al.The effects of apogee on shoot growth, secondary flowering, fire blight, fruit quality, yield and return bloom in ʻBartlett’ pear growing in California. J Hortic Sci Biotech2004; 79: 380–389.
17.
Lo GiudiceDWolfTKZoeckleinBW. Effects of prohexadione-calcium on grape yield components and fruit and wine composition. Am J Enol Vitic2004; 55: 73–83.
18.
MandemakerAJCuttingJGMSmithDB,et al.Effect of prohexadionecalcium on shoot growth, fruit set and retention in ‘Hass’ avocado in New Zealand. New Zealand Avoc Grow Assoc Ann Res Rep2005; 5: 35–42.
19.
BlackBL. Strawberry runner suppression with prohexadione-calcium. Acta Hortic2006; 708: 249–252.
20.
JacynaTLipaT. Direct and apparent residual effects of prohexadione-calcium applied to young cropping sweet cherry trees. Acta Agrobot2010; 63: 87–92.
21.
PalonenPMouhuK. Vegetative growth and flowering of primocane raspberry ʻAriadne’ as affected by prohexadione-calcium. HortSci2009; 44: 529–531.
22.
NenadićD. Removal of the first flush of canes – a new method in raspberry cultivation. Yugoslav Pomol [Uklanjanje Prvih Serija Izdanaka – nov Metod u Gajenju Maline. Jugoslovensko Voćarstvo-in Serbian]1986; 75/76: 539–543.
23.
Karaklajić-StajićŽNikolićMTomićJ, et al.Influence of ripeness stage on the rate of pigment degradation in blackberry fruits during frozen storage. Eur J Hortic Sci2017; 82: 198–203.
24.
Republic Hydro-Meteorological Bureau in Belgrade, Serbia Available from: http://www.hidmet.gov.rs (2024, accessed 20 November 2024).
25.
Karaklajic-StajicZTomicJPesakovicM, et al.Black queens of fruits: chemical composition of blackberry (Rubus subg. Rubus Watson) and black currant (Ribes nigrum L.) cultivars selected in Serbia. Foods2023; 12: 2775.
26.
EganHKirkRSSawyerR. Pearson's chemical analysis of foods. 8th ed. London, UK: Longman Group Ltd., 1981, p. 557.
27.
MoraisDRRottaEMSargiSC, et al.Proximate composition, mineral contents and fatty acid composition of the different parts and dried peels of tropical fruits cultivated in Brazil. J Braz Chem Soc2017; 28: 308–318.
28.
VeličkovićM. Opšte voćarstvo I: Biologija i ekologija drvenastih voćaka. [General pomology I: Biology and ecology of fruit trees]. Republic of Serbia: University of Belgrade, Faculty of Agriculture, 2004; Serbian.
29.
DeviKNVyasASSinghMS,et al.Effect of bioregulators on growth, yield and chemical constituents of soybean (Glycine max). J Agric Sci2011; 3: 151–159.
30.
XuT. Flavor and quality characteristics of two primocane-bearing blackberry cultivars as impacted by foliar and shade application. Doctoral dissertation, Virginia Tech, 2023.
31.
MilivojevićJRadivojevicDMaksimovićJD, et al.Does plant growth and yield affected by Prohexadione Ca cause changes in chemical fruit composition of ‘Loch Ness' and ‘Triple Crown’ blackberries?Eur J Hortic Sci2017; 82: 190–197.
32.
PoledicaMMMilivojevicJMRadivojevicDD,et al.Prohexadione-Ca and young cane removal treatments control growth, productivity, and fruit quality of the Willamette raspberry. Turk J Agric For2012; 36: 680–687.
33.
ByersRECarbaughDHCombsLD. The influence of prohexadione-calcium sprays on apple tree growth, chemical fruit thinning, and return bloom. J Am Pom Soc2004; 58: 111–117.
34.
HytönenTMouhuKKoivuI, et al.Planting year Prohexadione-calcium treatment increases the cropping potential and yield of strawberry. Acta Hortic2008; 842: 741–744.
35.
OwensCLStoverE. Vegetative growth and flowering of young apple trees in response to prohexadione-calcium. Hortic Sci1999; 34: 1194–1196.
36.
EvansJREvansRRRegusciCL,et al.Mode of action, metabolism, and uptake of BUS 125 W, prohexadione-calcium. HortSci1999; 34: 1200–1201.
37.
BasakALINA. The effect of prohexadione-Ca on shoot growth and cropping of young apple trees of ‘Jonagold’ cv. Roczniki Akademii Rolniczej w Poznaniu. Ogrodnictwo2007; 41: 261–268.
38.
ClineJA. Interactive effects of 6-BA, GA4+7, and prohexadione-calcium on Gala apples. Can J Plant Sci2017; 97: 632‒644.
39.
ReekieJYCHicklentonPR. Strawberry growth response to prohexadione–calcium. Strawberry research to 2001. In: Proceedings of the 5th North American Strawberry Conference, 2001, vol. 5, pp. 147–152.
40.
RouxCLemarquandAOrainG, et al.Effects of the plant growth regulator prohexadione-calcium and the SAR-inducer acibenzolar-S-methyl on the quality of apples at harvest. J Hortic Sci Biotechnol2006; 81: 139–145.
41.
LalMMirMIqbalU,et al.Influence of prohexadione-calcium and paclobutrazol on growth, yield and mineral content of pear cv. Clapp’s favourite. Int J Chem Stud2020; 8: 256–259.
42.
Gonzalo-DiagoAAvizcuriJOrtigosaN, et al.Prohexadione-calcium as a regulator of vine growth. Effect on the physical-chemical characteristics of wine grapes. In: Grapes: cultivation, varieties and nutritional uses. Hauppauge, NY: Nova Science Publishers, 2012, pp. 133–153.
43.
AvizcuriJGonzalo-diagoASanz-asensioJ, et al.Effect of cluster thinning and prohexadione calcium applications on phenolic composition and sensory properties of red wines. J Agric Food Chem2013; 61: 1124–1137.
44.
FlossEL. Fisiologia das plantas cultivadas: o estudo do que está por trás do que se vê. 5th ed. Rio Grande: Universidade de Passo Fundo, 2011.
45.
JingTLiJHeY, et al.Role of calcium nutrition in plant physiology: advances in research and insights into acidic soil conditions-A comprehensive review. Plant Phys Biochem2024; 210: 108602.
46.
KimYHAbdul Latif KhanALHamayunM, et al.Effects of prohexadione calcium on growth and gibberellins contents of Chrysanthemum morifolium R. cv Monalisa White. Sci Hortic2010; 123: 423–427.
47.
HakalaMLapveteläinenAHuopalahtiR, et al.Effects of varieties and cultivation conditions on the composition of strawberries. J Food Compost Anal2003; 16: 67–80.
48.
TosunIUstunNSTekgulerB. Physical and chemical changes during ripening of blackberry fruits. Sci Agri2008; 65: 87–90.
49.
Konić-RistićAŠavikinKZdunićG, et al.Biological activity and chemical composition of different berry juices. Food Chem2011; 125: 1412–1417.
50.
FalchiRBonghiCDrincovichMF, et al.Sugar metabolism in stone fruit: source-sink relationships and environmental and agronomical effects. Front Plant Sci2020; 11: 573982.
51.
SmithMRRaoIMMerchantA. Source-sink relationships in crop plants and their influence on yield development and nutritional quality. Front Plant Sci2018; 9: 1889.
52.
RomeroHPottDMVallarinoJG,et al.Metabolomics-based evaluation of crop quality changes as a consequence of climate change. Metabolites2021; 11: 61.
53.
BhattacharyaA.Role of plant growth hormones during soil water deficit: A review. In: Soil water deficit and physiological issues in plants. Singapore: Springer, 2021, pp.489–583. https://doi.org/10.1007/978-981-33-6276-5_6
