Genetic diversity analysis of some Vaccinium species in the Black Sea region of Türkiye using iPBS markers

Abstract

Background:

The genus Vaccinium is economically significant for its highly nutritious berries. Türkiye hosts wild genetic resources of Vaccinium, primarily distributed along the Black Sea region. These species are valuable for crop improvement, local food security, and require urgent conservation efforts.

Objectives:

This study analyzed the genetic diversity, population structure, and gene flow among wild Vaccinium species in Türkiye using iPBS retrotransposon markers.

Methods:

A total of 22 individuals from three wild Vaccinium species (V. arctostaphylos, V. myrtillus, V. uliginosum) and two domesticated cultivars were analyzed using 10 iPBS markers. Genetic diversity was assessed based on polymorphic bands, Shannon's index, and gene flow. Cluster analysis (UPGMA), principal component analysis (PCoA), and STRUCTURE analysis were performed to evaluate population structure.

Results:

Out of 312 bands, 177 (56%) were polymorphic, with the highest bands (32) produced by iPBS2391. Low genetic diversity (Shannon's index = 0.14–0.2) and moderate gene flow (0.3) were observed. AMOVA revealed 49% of variance within individuals and 51% among species. Distinct clusters were identified, though some individuals showed high admixture and heterozygosity, forming separate clusters.

Conclusion:

These findings provide insights into the genetic diversity and population structure of Turkish wild Vaccinium species, offering valuable information for conservation strategies and berry cultivation efforts worldwide.

Keywords

iPBS blueberry molecular characterization polymorphism

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References

Carbone

Mourgues

Perrotta

, et al. Advances in functional research of antioxidants and organoleptic traits in berry crops. BioFactors 2008; 34: 23–36.

Szajdek

Borowska

. Bioactive compounds and health-promoting properties of berry fruits: a review. Plant Foods Hum Nutr 2008; 63: 147–156.

Retamales

Hancock

JF.

Blueberries, vol. 27. Wallingford: CABI Publishing, 2018.

Ehlenfeldt

Prior

. Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. J Agric Food Chem 2001; 49: 2222–2227.

Deng

Yang

Yue

, et al. Influences of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Control 2014; 38: 184–191.

Milivojevic

Slatnar

Mikulic-Petkovsek

, et al. The influence of early yield on the accumulation of major taste and health-related compounds in black and red currant cultivars (Ribes spp.). J Agric Food Chem 2012; 60: 2682–2691.

Ballington

. Collection, utilization, and preservation of genetic resources in Vaccinium. HortScience 2001; 36: 213–220.

Hancock

Lyrene

Finn

, et al. Blueberries and cranberries. In: Hancock JF (ed) Temperate fruit crop breeding: germplasm to genomics. Dordrecht: Springer Netherlands, 2008, pp.115–150.

Cesoniene

Daubaras

Paulauskas

, et al. Morphological and genetic diversity of European cranberry (Vaccinium oxycoccos L., Ericaceae) clones in Lithuanian reserves. Acta Soc Bot Pol 2013; 82: 211–217.

10.

Sakhanokho

Rinehart

Stringer

, et al. Variation in nuclear DNA content and chromosome numbers in blueberry. Sci Hortic 2018; 233: 108–113.

11.

Ehlenfeldt

Ballington

. Vaccinium species of section Hemimyrtillus: their value to cultivated blueberry and approaches to utilization. Botany 2012; 90: 347–353.

12.

Bozdag

Sultana

Serçe

. Evaluation of some horticulturally important morphological traits of Turkish wild Vaccinium species. In: XXX international horticultural congress IHC2018: III international berry fruit symposium, 2018, pp.301–308.

13.

Iordăchescu

Asănică

Mihai

. RAPD, ISSR and SSR molecular markers applications in Vaccinium spp. Sci Pap Ser B Hortic 2022; 66: 87–93.

14.

Nowakowska

Pacholczak

. Analysis of genetic stability in the ex vitro rooted microcuttings of blueberry (Vaccinium corymbosum L.). Acta Sci Pol Hortorum Cultus 2017; 16: 19–27.

15.

Ehlenfeldt

Polashock

. Highly fertile intersectional blueberry hybrids of Vaccinium padifolium section Hemimyrtillus and V. corymbosum section Cyanococcus. J Am Soc Hortic Sci 2014; 139: 30–38.

16.

Carvalho

Matos

Carnide

. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort Sci (Prague) 2014; 41: 175–184.

17.

Zhang

Liu

, et al. Comparative transcriptome sequencing and de novo analysis of Vaccinium corymbosum during fruit and color development. BMC Plant Biol 2016; 16: 1–9.

18.

Sultana

Menzel

Heitkam

, et al. Bioinformatic and molecular analysis of satellite repeat diversity in Vaccinium genomes. Genes 2020; 11: 527.

19.

Çelik

Yüksek boylu maviyemiş (Highbush Blueberry) yetiştiriciliği (Gifimey Mesleki Yayınlar Serisi-III). 2012, p.150.

20.

Dellaporta

Wood

Hicks

. A plant DNA minipreparation: version II. Plant Mol Biol Rep 1983; 1: 19–21.

21.

Lyrene

Ballington

. Wide hybridization in Vaccinium. 1986.

22.

Ortiz

Vorsa

Bruederle

, et al. Occurrence of unreduced pollen in diploid blueberry species, Vaccinium sect. Cyanococcus. Theor Appl Genet 1992; 85: 55–60.

23.

Lyrene

Vorsa

Ballington

. Polyploidy and sexual polyploidization in the genus Vaccinium. Euphytica 2003; 133: 27–36.

24.

Sultana

Pascual-Díaz

Gers

, et al. Contribution to the knowledge of genome size evolution in edible blueberries (genus Vaccinium). J Berry Res 2020; 10: 243–257.

25.

Bjedov

Obratov-Petković

Misić

, et al. Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage. Silva Fenn 2015; 49: 1283.

26.

Haliloğlu

Türkoğlu

Öztürk

, et al. iPBS-Retrotransposon markers in the analysis of genetic diversity among common bean (Phaseolus vulgaris L.) germplasm from Türkiye. Genes 2022; 13: 1147.

27.

Demirel

Tındaş

Yavuz

, et al. Assessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker system. Plant Genet Resour 2018; 16: 137–145.

28.

Doungous

Kalendar

Filippova

, et al. Utility of iPBS retrotransposons markers for molecular characterization of African Gnetum species. Plant Biosyst 2020; 154: 587–592.

29.

Thiel-Egenter

Gugerli

Alvarez

, et al. Effects of species traits on the genetic diversity of high-mountain plants: a multi-species study across the Alps and the Carpathians. Glob Ecol Biogeogr 2009; 18: 78–87.

30.

Tang

Liu

Zhang

, et al. Genetic diversity and population structure of Amorphophallus albus, a plant species with extremely small populations (PSESP) endemic to dry-hot valley of Jinsha River. BMC Genet 2020; 21: 1–10.

31.

Hagenblad

Oliveira

Forsberg

, et al. Geographical distribution of genetic diversity in Secale landrace and wild accessions. BMC Plant Biol 2016; 16: 1–20.

32.

Nestby

Percival

Martinussen

, et al. The European blueberry (Vaccinium myrtillus L.) and the potential for cultivation. Eur J Plant Sci Biotechnol 2011; 5: 5–16.

33.

Alexander

Chalmandrier

Lenoir

, et al. Lags in the response of mountain plant communities to climate change. Glob Change Biol 2018; 24: 563–579.

34.

Swarup

Cargill

Crosby

, et al. Genetic diversity is indispensable for plant breeding to improve crops. Crop Sci 2021; 61: 839–852.

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