Hydrogen-rich water treatment maintains the quality and prolongs the shelf-life of postharvest table grapes by regulating respiratory and energy metabolisms
Restricted accessResearch articleFirst published online November, 2024
Hydrogen-rich water treatment maintains the quality and prolongs the shelf-life of postharvest table grapes by regulating respiratory and energy metabolisms
Table grapes suffer from a dramatic deterioration in quality, a reduction in shelf life and commercial value resulting from allivated postharvest respiratory metabolism and excessive energy consumption after harvest.
Objective
In this study, effects of hydrogen-rich water (HRW) on berry quality, respiratory metabolism, and energy level were investigated by multi concentration gradient tests (0% (control), 20%, 40%, 60%, 80%, and 100%).
Methods
To achieve this goal, by measuring fruit quality, respiratory metabolism, related enzyme activities, energy status, and other related indicators, to study its impact on postharvest fruit quality and further explore the mechanism of maintaining fruit quality after harvest.
Results
Compared to the control group, samples treated with HRW exhibited higher fruit firmness, total soluble solids (TSS), and titratable acids (TA), with the most prominent effect observed after treatment with 60% HRW. Compared with the control group, grapes treated with 60% HRW exhibited more effective regulation in the expression of GPI, AOX, SDH1, COX6a, G6DH, 6PGD, NADK, and AGPase gene, which helped maintain normal respiratory metabolism and energy levels in the fruit samples.
Conclusions
Our results showed that HRW treatment could potentially maintain the quality and prolong the shelf life of postharvest grapes by regulating gene expression and the activity of key enzymes involved in respiratory and energy metabolisms.
GuoDLZhaoHLZhangGH, et al.Transmission of early ripening trait related loci in grapevines from backbone cultivar Pearl of Csaba to its descendants. Sci Hortic2018; 244: 151–156.
2.
AlstonJMSambucciO. Grapes in the world economy. In: Dario Cantu and M. Andrew Walker, editors. Compendium of plant genomes. New York, NY: Springer Cham, 2019, pp. 1–24.
3.
MengXLiBLiuJ, et al.Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chem2008; 106: 501–508.
4.
TelesCSBenedettiBCGublerWD, et al.Prestorage application of high carbon dioxide combined with controlled atmosphere storage as a dual approach to control Botrytis cinerea in organic ‘flame Seedless’ and ‘crimson Seedless’ table grapes. Postharvest Biol Technol2014; 89: 32–39.
5.
RomanazziGGablerFMSmilanickJL. Preharvest chitosan and postharvest UV irradiation treatments suppress gray mold of table grapes. Plant Dis2006; 90: 445–450.
6.
SharmaPSharmaSRMittalTC. Effects and application of ionizing radiation on fruits and vegetables: a review. J Agric Eng2020; 57: 97–126.
7.
ZhuSLiangYMuL, et al.1-Methylcyclopropene On fruit quality of se-enriched grape (Vitis vinifera L.) during shelf life period. Agronomy2020; 10: 1411.
8.
ZutahyYLichterAKaplunovT, et al.Extended storage of ‘red globe’ grapes in modified SO2 generating pads. Postharvest Biol Technol2008; 50: 12–17.
9.
LiuHNPeiMSWeiTL, et al.ROS Scavenger hypotaurine delays postharvest softening of ‘Kyoho’ grape by regulating pectin and cell metabolism pathway. Postharvest Biol Technol2022; 186: 111833.
10.
SunYDGuoDLYangSD, et al.Melatonin treatment improves the shelf-life and postharvest quality of table grape (Vitis Labrusca L. Cv. “Fengzao”). J Berry Res2020; 10: 665–676.
11.
HuangCSKawamuraTPengX, et al.Hydrogen inhalation reduced epithelial apoptosis in ventilator-induced lung injury via a mechanism involving nuclear factor-kappa B activation. Biochem Biophys Res Commun2011; 408: 253–258.
12.
DongWShiLLiS, et al.Hydrogen-rich water delays fruit softening and prolongs shelf life of postharvest okras. Food Chem2023; 399: 133997.
13.
HuHLiPWangY, et al.Hydrogen-rich water delays postharvest ripening and senescence of kiwifruit. Food Chem2014; 156: 100–109.
14.
ZhaoXMMengXMLiWX, et al.Effect of hydrogen-rich water and slightly acidic electrolyzed water treatments on storage and preservation of fresh-cut kiwifruit. J Food Meas Charact2021; 15: 5203–5210.
15.
ChenHZhangJHaoH, et al.Hydrogen-rich water increases postharvest quality by enhancing antioxidant capacity in Hypsizygus marmoreus. AMB Express2017; 7: 21.
16.
DongWCaoSZhouQ, et al.Hydrogen-rich water treatment increased several phytohormones and prolonged the shelf life in postharvest okras. Front Plant Sci2023; 14: 1108515.
17.
DingBZhuDYaoQ, et al.Hydrogen-rich water treatment improves fresh-cut Gastrodia elata quality by regulating reactive oxygen species metabolism and energy metabolism during low temperature storage. Front Sustain Food Syst2023; 7: 1274701.
18.
DingXYaoQZhuD, et al.Hydrogen-rich water maintains the quality of Rosa roxburghii by regulating AsA biosynthesis and regeneration. Postharvest Biol Technol2023; 195: 112136.
19.
AnRLuoSZhouH, et al.Effects of hydrogen-rich water combined with vacuum precooling on the senescence and antioxidant capacity of pakchoi (Brassica rapa subsp. Chinensis). Sci Hortic2021; 289: 110469.
20.
DongBZhuDYaoQ, et al.Hydrogen-rich water treatment maintains the quality of Rosa sterilis fruit by regulating antioxidant capacity and energy metabolism. LWT2022; 161: 113361.
21.
SunJLinHZhangS, et al.The roles of ROS production-scavenging system in Lasiodiplodia theobromae (Pat.) Griff. & Maubl. -induced pericarp browning and disease development of harvested longan fruit. Food Chem2018; 247: 16–22.
22.
JiaZBaoYZhaoY, et al.Cold shock treatment enhances cold tolerance in peach fruit through modulating PpbZIP9 and PpVIP1-mediated respiratory metabolism. Postharvest Biol Technol2023; 204: 112421.
23.
ZhangWZhaoHZhangJ, et al.Different molecular weights chitosan coatings delay the senescence of postharvest nectarine fruit in relation to changes of redox state and respiratory pathway metabolism. Food Chem2019; 289: 160–168.
24.
Jardim-MessederDCaverzanARauberR, et al.Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses. New Phytol2015; 208: 776–789.
25.
LiDLimwachiranonJLiL, et al.Involvement of energy metabolism to chilling tolerance induced by hydrogen sulfide in cold-stored banana fruit. Food Chem2016; 208: 272–278.
26.
LinLLinYLinH, et al.Comparison between ‘Fuyan’ and ‘Dongbi’ longans in aril breakdown and respiration metabolism. Postharvest Biol Technol2019; 153: 176–182.
27.
YuYHLiXFYangSD, et al.CPPU-induced changes in energy status and respiration metabolism of grape young berry development in relation to Berry setting. Sci Hortic2021; 283: 110084.
28.
LinYLinYLinH, et al.Application of propyl gallate alleviates pericarp browning in harvested longan fruit by modulating metabolisms of respiration and energy. Food Chem2018; 240: 863–869.
29.
ChenYLinHJiangY, et al.Phomopsis longanae chi-induced pericarp browning and disease development of harvested longan fruit in association with energy status. Postharvest Biol Technol2014; 93: 24–28.
30.
PeiMSLiuHNYuYH, et al.Folic acid delays postharvest quality deterioration of table grape by regulating cell wall metabolism-associated hub WRKY31 transcription factor. Postharvest Biol Technol2023; 197: 112207.
31.
LinYLinHChenY, et al.The role of ROS-induced change of respiratory metabolism in pulp breakdown development of longan fruit during storage. Food Chem2020; 305: 125439.
32.
LiuSJingGZhuS. Nitric oxide (NO) involved in antioxidant enzyme gene regulation to delay mitochondrial damage in peach fruit. Postharvest Biol Technol2022; 192: 111993.
33.
HuanCHanSJiangL, , et al.Postharvest hot air and hot water treatments affect the antioxidant system in peach fruit during refrigerated storage. Postharvest Biol Technol. 2017; 126: 1–14.
34.
LiuYLiYBiY, et al.Induction of defense response against Alternaria rot in Zaosu pear fruit by exogenous L-lysine through regulating ROS metabolism and activating defense-related proteins. Postharvest Biol Technol2021; 179: 111567.
35.
SaltveitME.Respiratory metabolism. In: Elhadi M. Yahia, editor. Postharvest physiology and biochemistry of fruits and vegetables. Cambridge, UK: Woodhead Publishing, 2019, pp. 73–91.
36.
LinYChenMLinH, et al.Phomopsis longanae-induced pericarp browning and disease development of longan fruit can be alleviated or aggravated by regulation of ATP-mediated membrane lipid metabolism. Food Chem2018; 269: 644–651.
37.
LinYXLinYFChenYH, et al.Hydrogen peroxide induced changes in energy Status and respiration metabolism of harvested Longan fruit in relation to pericarp browning. J Agric Food Chem2016; 64: 4627–4632.
38.
TanXLFanZQZengZX, et al.Exogenous melatonin maintains leaf quality of postharvest Chinese flowering cabbage by modulating respiratory metabolism and energy status. Postharvest Biol Technol2021; 177: 111524.
39.
ZhangYXWangYChenFH, et al.Exogenous nitric oxide inhibits the respiratory metabolism of postharvest wax apple fruit and its role in the delayed cottony softening. Sci Hortic2023; 317: 112043.
40.
LiuHNPeiMSWeiTL, et al.Molecular cloning and expression analysis of hydrogen peroxide sensors under H2O2 and ROS inhibitor treatment in ‘Kyoho’ grape berry. Postharvest Biol Technol2021; 180: 111617.
41.
WuZTuMYangX, et al.Effect of cutting on the reactive oxygen species accumulation and energy change in postharvest melon fruit during storage. Sci Hortic2019; 257: 108752.
42.
ChenYSunJLinH, et al.Salicylic acid reduces the incidence of Phomopsis longanae chi infection in harvested longan fruit by affecting the energy status and respiratory metabolism. Postharvest Biol Technol2020; 160: 111035.
43.
LiuTWangHKuangJ, et al.Short-term anaerobic, pure oxygen and refrigerated storage conditions affect the energy status and selective gene expression in litchi fruit. LWT-Food Sci Technol2015; 60: 1254–1261.
44.
WangTHuMYuanD, et al.Melatonin alleviates pericarp browning in litchi fruit by regulating membrane lipid and energy metabolisms. Postharvest Biol Technol2020; 160: 111066.
