Erratum to “Astrocyte-Selective Volume Increase in Elevated Extracellular Potassium Conditions Is Mediated by the Na + /K + ATPase and Occurs Independently of Aquaporin 4”

Walch, E., Murphy, T. R., Cuvelier, N., Aldoghmi, M., Morozova, C., Donohue, J., Young, G., Samant, A., Garcia, S., Alvarez, C., Bilas, A., Davila, D., Binder, D. K., & Fiacco, T. A. (2020). Astrocyte-Selective Volume Increase in Elevated Extracellular Potassium Conditions Is Mediated by the Na⁺/K⁺ ATPase and Occurs Independently of Aquaporin 4. ASN Neuro. https://doi.org/10.1177/1759091420967152

In the published version of Figure 8A, the scale bar is not visible. The correct version of the figure is as given below:

Figure 8.

Block of Neuronal Firing Has No Effect on Astrocyte Swelling in Elevated Potassium. (A) Representative recording of a CA1 pyramidal neuron in 10.5 mM [K⁺]_o. Application of 10.5 mM [K⁺]_o depolarized the cell by ∼25 mV and resulted in generation of frequent action potentials within ∼30 s. In control ACSF (2.5 mM [K⁺]_o), action potentials were absent in this cell, both during the baseline and wash periods. (B) On average, neurons rested at –67.2 mV and depolarized to –48.3 mV in the presence of 10.5 mM [K⁺]_o. (C) Application of 10.5 mM [K⁺]_o significantly increased the frequency of action potentials in CA1 pyramidal cells on average. For (B) and (C), n = 11 neurons, **p < .01, ***p < .001. Pseudocolor images of astrocytes (D, E) depict cell volume at baseline overlaid with images taken after 5 min in isoosmolar 10.5 mM [K⁺]_o (D) or 10.5 mM [K⁺]_o plus 1 µM TTX (E) (left panels). “Wash” images (right panels) show cell baseline volume overlaid with volume post-^[K⁺]_o reperfusion in control ACSF. The magenta outline (arrows) shows expansion of the cell soma over baseline volume. (F) Coapplication of 1 µM TTX with 10.5 mM [K⁺]_o had no significant effect on astrocyte swelling compared with 10.5 mM [K⁺]_o alone (control n = 8; + TTX n = 10). TTX, like other antagonists, was applied 10 min prior to addition of 10.5 mM [K⁺]_o + TTX and was present throughout the electrophysiology recordings and imaging measurements.