This article deals with the design of an integrated giant magnetoimpedance biochip combined with a sandwich immunoassay for the detection of alpha-fetoprotein, and we have offered the possible physical origins of the enhanced giant magnetoimpedance responses caused by Dynabeads. The giant-magnetoimpedance-based magnetic immunoassay showed a good sensitivity and specificity for the detection of 3 ng/mL alpha-fetoprotein. We also observed that there was significant difference in detection sensitivity between two giant magnetoimpedance biochips, which is probably related to the electroplating instability. Such an integrated giant magnetoimpedance biochip could be extended for the detection toward a wide spectrum of target biomolecules and shows promising potential for clinical applications.
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