The Tumor Markers Tpa,Tps,Tpa Cyk and Cyfra 21–1 React Differently with the Keratins 8,18 and 19

Abstract

The commercially available tumor marker tests TPA, TPS, TPA_CYK and CYFRA 21–1 react with simple epithelium keratins. From clinical studies it can be deduced that the pattern of keratin recognition must be different for each of these tests. We therefore studied the reactivity of the keratin fragment combinations K8/K18 and K8/K19 in the different tests and determined the reactivity of the corresponding soluble antibodies with purified keratin 8, 18 and 19 in immunoblots. TPS and CYFRA 21–1 were found to distinguish clearly between the keratin fragment combinations K8/K18 (TPS) and K8/K19 (CYFRA 21–1). TPA and TPA_CYK reacted with both combinations, however, with different intensities. On immunoblots the CYFRA 21–1 antibodies reacted exclusively with K19, whereas the antibodies of the other assays reacted with at least 2 of the keratins investigated.

Keywords

TPA TPS CYFRA 21–1 Keratins Tumor markers

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References

Moll

, Franke

W.W.

, Schiller

D.L.

, Geibzer

, Krepier

The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.

Cell 1982; 31: 11–24

Fuchs

, Grace

M.P.

, Kim

K.H.

, Marchuk

Differential expression of two classes of keratins in normal and malignant epithelial cells and their evolutionary conservation. In: Levin

A.J.

, Vande Woude

G.F.

, Tropp

W.C.

, Wason

J.D.

, eds. Cancer cells, Vol. 1; The transformed phenotype. Cold Spring Harbour, N.Y: Cold Spring Harbour Laboratory 1984; 161–8.

Moll

Differenzierung und Entdifferenzierung im Spiegel der Intermediärfilament-Expression: Untersuchungen an normalen, alterierten und malignen Epithelien mit Betonung der Cytokeratine.

Verh Dtsch Ges Path 1991; 75: 446–59.

Hatzfeld

, Franke

W.W.

Pair formation and promiscuity of cytokeratins: Formation in vitro of heterotypic complexes and intermediate-sized filaments by homologous and heterologous recombinations of purified polypeptides.

J Cell Biol 1985; 101: 1826–41.

Achtstätter

, Hatzfeld

, Quinlan

R.A.

, Parmelee

D.C.

, Franke

W.W.

Separation of cytokeratin polypeptides by gel electrophoretic and chromatographic techniques and their identification by immunoblotting.

Meth in Enzymol 1986; 134: 355–71.

Mellerick

D.M.

, Osborn

, Weber

On the nature of serological TPA; monoclonal keratin 8, 18 and 19 antibodies react differently with TPA prepared from human cultured cells and TPA in serum.

Oncogene 1990; 5: 1007–17.

Stieber

, Bodenmüller

, Banauch

Cytokeratin 19 fragments: A new marker for non-small-cell lung cancer.

Clin Biochem 1993; 26: 301–4.

Sundström

, Stigbrand

A two-site enzyme-linked immunosorbent assay for cytokeratin 8.

Int J Cancer 1990; 46: 604–7.

Stieber

, Dienemann

, Hasholzner

, Zimmermann

, Hofmann

, Fateh-Moghadam

Comparison of CYFRA 21–1, TPA and TPS in lung cancer.

J Tumor Marker Oncol 1993; 8: 48, abstract.

10.

Ferdieghini

, Gadducci

, Annicchiarico

Serum TPA, TPS, cytokeratins 8–18 and CYFRA 21–1 levels in uterine malignancies.

J Tumor Marker Oncol 1993; 8: 46 (abstract).

11.

Gershoni

J.M.

, Palade

G.E.

Protein blotting: Principles and applications.

Annal Biochem 1983; 131: 1–15.

12.

Bodenmüller

, Ofenloch-Hähnle

, Banauch

, Dessauer

Lung cancer associated cytokeratin 19 fragments: Biochemical background of the serum assay CYFRA 21–1.

J Tumor Marker Oncol 1993; 8: 44 (abstract).

13.

Björklund

Tumor markers TPA, TPA-S and cytokeratins.

Tumordiagn u Ther 1992; 13: 78–80.