Distribution,Kinetics and Immunoscintigraphy of 131-I Labelled Intact Antifibrinogen-fibrin Antibody (AbFbg) and its F(ab') 2 Fragment

Abstract

131-I-labelled anti fibrin-fibrinogen antibody (AbFbg) was compared with its F(ab')₂ fragment in distribution studies and by immunoscintigraphy with a view to tumour visualization in tumour bearing rats. The distribution studies indicated that the intact antibody is more concentrated in tumour tissue than the F(ab')₂ fragment. By 168h after injection, when tumour-to-tissue ratios were highest in the majority of tissues, the tumour concentration of intact antibody was 3 to 4 times that of the F(ab')₂ fragment. The intact antibody is more suitable than the F(ab')₂ fragment for tumour imaging especially in the abdominal region where the highest tumour-to tissue ratios were obtained with intact antibody in liver, spleen, intestines and kidneys.