Assessment of the accuracy of diagnostic procedures is made independent of diagnostic criteria by means of a receiver-operating-characteristics (ROC) curve. We performed ROC analysis for the major serum antiproteases: alpha-1-antitrypsin (A1AT) and alpha-2-macroglobulin (A2M), in 99 cancer patients compared with 71 normal individuals. A1AT and A2M were significantly higher in cancer patients (p < 0.0005). By comparing true positive and false positive rates for different serum levels, ROC analysis showed that serum A1AT quantification seems more useful in clinical practice than serum A2M.
DabbousM.K., RobertsA.N., BrinkleyB.Collagenase and neutral protease activities in cultures of rabbit VX-2 carcinoma.Cancer Res1977; 37: 3537–40.
2.
StevenF.S., ItzhakiS.Evidence for a latent form of collagenase extracted from rabbit tumour cells.Biochim Biophys Acta1977; 496: 241–7.
3.
LiottaL.A., AbeS., RobeyP.G., MartinG.R.Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor.Proc Natl Acad Sci USA1979; 76: 2268.
4.
SylvenB.Biochemical factors involved in the detachment from tumors.Schweiz Md Wochenschr1974; 104: 258–63.
5.
SharpH.L.Current status of alpha-1-antitrypsin, a protease inhibitor, in gastrointestinal disease.Gastroenterology1976; 70: 611–21.
RombachJ.J., ColletteH.J.A., SlotboomB.J., De WardF.ROC analysis of breast cancer diagnosis. 4th Breast Cancer Working Conference EORTC. London, July 1987.
10.
ManciniG., CarbonaraA.O., HeremansJ.F.Immunochemical quantitation of antigens by single radial immunodiffusion.Immunochemistry1965; 2: 235–54.
11.
MillanJ., LorenzoA., RodriguezP., GallurtP., RomeroJ., SenraA.Variations of major acute phase alpha-1-protein response in normal, non-malignant and malignant states. In: Peeters H, ed. Protides of the biological fluids.Oxford: Pergamon Press,1986; 34: 427–30.
12.
BorekE.Toward a universal tumour marker.Tumour Biology1984; 5: 1–14.
13.
BagshaweK.D.Tumour markers - Where do we go from here?Br J Cancer1983; 48: 167–75.
14.
CooperE.H., StoneJ.Acute phase reactant proteins in cancer.Adv Cancer Res1979; 30: 1–7.
15.
ChawlaR.K., RauschD.J., MillerF.W., VoglerW.R., LawsonD.H.Abnormal profile of serum proteinase inhibitors in cancer patients.Cancer Res1984; 44: 2718–23.
16.
LamoureauxG., MandevilleR., PoissonR., Legault-PoissonS., JolicoeurR.Biologic markers and breast cancer: a multiparametric study. I. Increased serum protein levels.Cancer1982; 49: 502–12.
17.
Lorenzo PeñuelasA.Utilización clínica de los inhibidores séricos de proteasas en los enfermos tumorales. Tesis Doctoral. Universidad de Cádiz, 1986.
