Monoclonal Antibodies to αDna Polymerase as a Marker of Cell Proliferative Activity

Abstract

A hybridoma cell line (5F) secreting monoclonal antibodies directed to αDNA polymerase has been developed. Kinetic studies on peripheral blood lymphocytes stimulated with mitogen and human colon cancer cell lines established in vitro were made by the two autoradiographic techniques of Thymidine Labelling Inde and Primer-dependent αDNA polymerase Labelling Index and the immunoperoxidase assay (PAP) with monoclonal antibody to αDNA polymerase. We demonstrated the exclusively intranuclear presence of αDNA polymerase in lymphocytes induced to proliferate and actively growing colon cancer cells in contrast with the cytoplasmic distribution of the enzyme in resting stage populations. The feasibility of using monoclonal antibodies to αDNA polymerase to determine cell growth fraction was evaluated.