Confidence Interval Estimation for DNA and mRNA Concentration by Real-Time PCR: A New Environment for an Old Theorem

Orlando C. , Pinzani P. , Pazzagli M. . Developments in quantitative PCR. Clin Chem Lab Med 1998; 36: 255–69.

Bustin S.A. . Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000; 25: 169–93.

Ginzinger D.G. . Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 2002; 30: 503–12.

Jung R. , Soondrum K. , Neumaier M. . Quantitative PCR. Clin Chem Lab Med 2000; 38: 833–6.

Bustin S.A. . Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 2002; 29: 23–39.

Fieller E.C. . The biological standardization of insulin. J R Stat Soc 1940; Suppl 7: 1–64.

SAS Institute Inc., SAS OnlineDoc®, Version 8, Cary, NC: SAS Institute Inc., 1999.