Effect of Oxymatrine Combined with Low Dose 5-FU on Lymphatic Vessel and Microvascular Endothelial Cell Growth of Gastric Cancer in a Severe Combined Immunodeficient Mouse Orthotopic Implantation Model
Free accessResearch articleFirst published online January, 2011
Effect of Oxymatrine Combined with Low Dose 5-FU on Lymphatic Vessel and Microvascular Endothelial Cell Growth of Gastric Cancer in a Severe Combined Immunodeficient Mouse Orthotopic Implantation Model
In this study, we explored the effect of Oxymatrine combined with low dose 5-Fu on lymphatic vessel and vascular endothelial growth factor of orthotopic implantated gastric cancer in severe combined immunodeficient (SCID) nude mice. Human gastric cancer cell line SGC-7901 was orthotopically implanted into the gastric tract of nude mice. Nude mice were treated with normal saline (control group), low dose 5-Fu, oxymatrine, oxymatrine combined with low dose 5-Fu using intraperitoneal injection. The expression of LVD, VEGF-C, VEGF-D, VEGF-R-3 and their Ct were analyzed in a severe combined immunodeficient mouse orthotopic implantatation gastric cancer model. We found that oxymatrine combined with low dose 5-Fu could decrease LVD and inhibit VEGF expression by a synergistic effect in SCID nude mouse orthotopic implantatation gastric cancer model.
PorteraCAJrBermanRSEllisLM. Molecular determinants of gastric cancer metastasis. Surg Oncol1998; 7:183–95.
2.
FidlerIJ. Critical determinants of metastasis. Semin Cancer Biol2002; 12:89–96.
3.
ZhuJSShengBChenJLChenGQYuXHYuHFZhuZM. Molecule action mechanisms of Oxymatrine combined with low dose 5-Fu on human gastric cancer SGC-7901 cells in vivo or in vitro. World J Gastroenterol2003; 10:2366–69.
4.
ZhuJSJiangXHChengWX. Studies on peritoneal chemotherapy treatment of gastric cancer ascites and correlative effects. Hepato Gastroenterol2000; 15: 108.
5.
WangZNXuHMJiangLZhouXLuCZhangX. Expression of survivin aRNA in peritoneal lavage fluid from patients with gastric cancer. Chin Med J2004; 117:1210–17.
BÖhmMWielandISchützeKRübbenH. Microbeam moment: Non-contact laser microdissection of membrane-mounted native tissue. Am J Pathol1997; 151:63–67.
8.
LiottaLPetricoinE. Molecular profiling of human cancer. Nat Rev2000; 1:48–56.
9.
WangLZhuJSSongMQChenGQChenJL. Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray. World J Gastroenterol2006; 12: 6949–54.
10.
VersteegHHSpekCAPeppelenboschMPRichelDJ. Tissue factor and cancer metastasis: The role of intracellular and extracellular signaling pathways. Mol Med2004; 10:6–11.
11.
KakinumaTHwangST. Chemokines, chemokine receptors, and cancer metastasis. J Leukoc Biol2006; 79:639–51.
12.
CuiYYingYvan HasseltA. OPCML is a broad tumor suppressor for multiple carcinomas and lymphomas with frequently epigenetic inactivation. PLoS ONE2008; 3:e2990.
13.
YeFZhangSFXieXLuWG. OPCML gene promoter methylation and gene expression in tumor and stroma cells of invasive cervical carcinoma. Cancer Invest2008; 26:569–74.
14.
HayashidaTUedaMAiuraKTadaHOnizukaMSenoMYamadaHKitajimaM. Anti-angiogenic effect of an insertional fusion protein of human basic fibroblast growth factor and ribonuclease-1. Protein Eng Des Sci2005; 18:321–7.
15.
MahajanNPWhangYEMohlerJLEarpHS. Activated tyrosine kinase Ackl promotes prostate tumorigenesis: Role of Ackl in polyubiquitination of tumor suppressor. Cancer Res2005; 65:10514–23.
16.
LynchGKohlerSLeserJBeilMGarcia-MarinLJLutzMP. The tyrosine kinase Yes regulates actin structure and secretion during pancreatic acinar cell damage in rats. Pflugers Arch2004; 447:445–51.
17.
NtougkosERushRScottDFrankenbergTGabraHSmythJFSellarGC. The IgLON family in epithelial ovarian cancer: Expression profiles and clinicopathologic correlates. Clin Cancer Res2005; 11:5764–68.
18.
ReedJEDunnJRdu PlessisDG. Expression of cellular adhesion molecule ‘OPCML’ is down-regulated in gliomas and other brain tumours. Neuropathol Appl Neurobiol2007; 33:77–85.
19.
SellarGCWattKPRabiaszGJ. OPCML at 11q25 is epigenetically inactivated and has tumor-suppressor function in epithelial ovarian cancer. Nat Genet2003; 34:337–43.
20.
GuoWShangFLiuQ. Differential regulation of components of the ubiquitin-proteasome pathway during lens cell differentiation. Invest Ophthalmol Vis Sci2004; 4:1194–01.
21.
WeiWAyadNGWanY. Degradation of the SCF component Skp2 in cell-cycle phase G1 by the anaphase-promoting complex. Nature2004; 6979: 194–8.
22.
KudoYKitajimaSSatoS. Transfection of p27(Kip 1) threonine residue 187 mutant type gene, which is not influenced by ubiquitin-mediated degradation, induces cell cycle arrest in oral squamous cell carcinoma cells. Oncology2002; 4:398–404.
23.
DefattaRJDe BenedettiA. Translational up-regulation of Yes accompanies eIF4E-mediated oncogenic transformation. Int J Oncol2000; 23: 1709–13.
24.
LawsonCGoupilSLeclercP. Increased activity of the human sperm tyrosine kinase sre by the camp-dependent pathway in the presence of calcium. Biol Reprod2008; 79:657–66.
25.
BarracloughJHodgkinsonCHoggADiveCWelmanA. Increases in c-Yes expression level and activity promote motility but not proliferation of human colorectal carcinoma cells. Neoplasia2007; 9:745–54.
26.
MahajanNPLiuYMajumderSWarrenMRParkerCEMohlerJLEarpHSWhangYE. Activated Cdc42-associated kinase Ack1 promotes prostate cancer progression via androgen receptor tyrosine phosphorylation. Proc Natl Acad Sci2007; 104: 8438–43.
27.
ModzelewskaKNewmanLPDesaiRKeelyPJ. Ack1 mediates Cdc42-dependent cell migration and signaling to p130Cas. J Biol Chem2006; 281:37527–35.
28.
CastiglioneFTaddeiABuccolieroAMGarbiniFGheriCFFreschiGBechiPDegl'InnocentiDRTaddeiGL. TNM staging and T-cell receptor gamma expression in gastric adenocarcinoma. Correlation with disease progression?Tumori2008; 94:384–88.
29.
IddingsDBilchikA. The biologic significance of micrometastatic disease and sentinel lymph node technology on colorectal cancer. J Surg Oncol2007; 96:671–7.
