Proteolytic Modifications of Lipoprotein(a): Potential Relevance to its Postulated Atherothrombogenic Role

Lawn RM , Scanu AM . Lipoprotein(a). In: Fuster V , Ross R , Topol EJ , eds. Coronary artery disease. Philadelphia: Lippincott-Raven Publishers, 1996:151–61.

Utermann G . Lipoprotein(a). In: Scriver CR , Beaudet AL , Sly WS , Valle D , eds. Metabolic and molecular bases of inherited disease. 7th ed., vol. 2, New York: McGraw Hill, 1995:1887–912.

Jauhiainen M , Koskinen P , Ehnholm C , Frick MH , Manttari M , Manninen V , Huttunen JK . Lipoprotein(a) and coronary heart disease risk: a nested case-control study of the Helsinki Heart Study participants. Atherosclerosis 1991;89:59–67.

Ridker PM , Hennekens CH , Stampfer MJ . A prospective study of lipoprotein(a) and the risk of myocardial infarction. JAMA 1993;270:2195–99.

Scanu AM , Fless GM . Lipoprotein(a): heterogeneity and biological relevance. J Clin Invest 1990;85:1709–15.

Lawn RM , Wade DP , Hammer RE , Chiesa G , Verstuyft JG , Rubin EM . Atherogenesis in transgenic mice expressing human apolipoprotein(a). Nature 1992;360:670–2.

Mancini FP , Newland DL , Mooser V , Murata J , Marcovina S , Young SG , Hammer RE , Sanan DA , Hobbs HH . Relative contributions of apolipoprotein(a) and apolipoprotein B to the development of fatty lesions in the proximal aorta of mice.

Hajjar HA , Gavish D , Breslow JL , Nachman RL . Lipoprotein(a) modulation of endothelial cell surface fibrinolysis and its potential role in atherosclerosis. Nature 1989;339:303–5.

Miles LA , Fless GM , Levine EG , Scanu AM , Plow EF . A potential role for the thrombotic risks associated with lipoprotein(a). Nature 1989;339:301–3.

McCormick SPA , Linton MF , Hobbs HH , Taylor S , Curtiss LK , Young SG . Expression of human apolipoprotein B90 in transgenic mice. Demonstration that apolipoprotein B90 lacks the structural requirements to form lipoprotein(a). J Biol Chem 1994;269:24284–9.

Callow MJ , Rubin EM . Site-specific mutagenesis demonstrates that cysteine 4326 of apolipoprotein B is required for covalent linkage with apolipoprotein(a) in vivo. J Biol Chem 1995;270:23914–7.

Chiesa G , Hobbs HH , Koschinsky ML , Lawn RM , Maika SD , Hammer RE . Reconstitution of lipoprotein(a) by infusion of human low density lipoprotein into transgenic mice expressing human apolipoprotein(a). J Biol Chem 1992;267:24369–74.

Edelstein C , Davidson NO , Scanu AM . Oleates stimulates the formation of triglyceride-rich particles containing apo B100-apo(a) in long-term primary cultures of human hepatoyctes. Chem Phys Lipids 1994;67/68:135–43.

Bonen D , Hausman A , Hadjiagapiou C , Skarosi S , Davidson N . Expression of a recombinant apolipoprotein(a) in HepG2 cells. J Biol Chem 1997;272:5659–67.

White A , Rainwater D , Lanford R . Intracellular maturation of apolipoprotein(a) and assembly of lipoprotein(a) in primary baboon hepatoyctes. J Lipid Res 1993;34:509–17.

Scanu AM , Miles L , Fless GM , Pfaffinger D , Eisenbart J , Jackson E , Hoover-Plow JL , Brunck T , Plow EF . Rhesus monkey lipoprotein(a) binds to Iysine sepharose and U937 monocytoid cells less efficiently than human lipoprotein(a). J Clin Invest 1993;91:283–91.

Edelstein CM , Mandala D , Pfaffinger D , Scanu AM . Determinants of lipoprotein(a) assembly: a study of wild-type and mutant apolipoprotein(a) phenotypes isolated from human and rhesus monkey lipoprotein(a) under mild reductive conditions. Biochemistry 1995:34:16483–92.

Ernst A , Helmhold M , Brunner C , Pethoschramm A , Armstrong VW , Muller HJ . Identification of two functionally distinct lysine binding sites in kringle 37 and in kringles 32-36 of human apolipoprotein(a). J Biol Chem 1995;270:6227–34.

Klezovitch O , Edelstein C , Scanu AM . Evidence that the fibrinogen binding domain of apo(a) is outside the lysine binding site (LBS) of kringle IV-10. A study involving naturally-occurring Iysine binding defective lipoprotein(a) phenotypes. J Clin Invest 1996;98:185–91.

Salonen EM , Jauhiainen M , Zardi L , Vaheri A , Ehnholm C . Lipoprotein(a) binds to fibronectin and has serine proteinase activity capable of cleaving it. EMBO J 1989;8:4035–40.

Edelstein C , Italia JA , Klezovitch O , Scanu AM . Functional and metabolic differences between elastase-generated fragments of human lipoprotein(a) and apolipoprotein(a). J Lipid Res 1996;37:1786–801.

Klezovitch O , Edelstein C , Zhu L , Scanu AM . Apolipoprotein(a) binds via its C-terminal domain to the protein core of the proteoglycan-decorin. Implications for the retention of lipoprotein(a) in atherosclerotic lesions. J Biol Chem 1998;37:23856–65.

Edelstein C , Italia JA , Scanu AM . Polymorphonuclear cells isolated from human peripheral blood cleave lipoprotein(a) and apolipoprotein(a) at multiple interkringle sites via the enzyme elastase. J Biol Chem 1997;272:11079–87.

Edelstein C , Italia JA , Klezovitch ON , Scanu AM . Metalloproteinase-2 cleaves lipoprotein(a) and apolipoprotein(a) in the linker region between kringles IV-4 and IV-5. Circulation 1997;96(Suppl):I-111.

Mooser V , Marcovina SM , White AL , Hobbs HH . Kringle-containing fragments of apolipoprotein(a) circulate in human plasma and are excreted into the urine. J Clin Invest 1996;98:2414–24.

Bader G , Edelstein C , Shamburek RD , Nishiwak M , Nazih H , Schwartz C , Scanu AM , Brewer HB . Lp(a): comparison of the in vivo metabolism of apo(a) and Lp(a) in man. Circulation 1996;94(Suppl):36.

Argraves KM , Kozarsky KF , Fallon JT , Harpel PC , Strickland DK . The atherogenic lipoprotein Lp(a) is internalized and degraded in a process mediated by the VLDL receptor. J Clin Invest 1997;100:2170–81.

Pepin JM , O'Neil JA , Hoff NF . Quantification of apo(a) and apo B in human atherosclerotic lesions. J Lipid Res 1991;32:317–27.

Hoff HF , O'Neil J , Yashiro A . Partial characterization of lipoproteins containing apo(a) in human atherosclerotic lesions. J Lipid Res 1993;34:789–98.

Cushing GL , Gaubatz JW , Nava ML , Burdick BJ , Bocan TMA , Guyton JR , Weilbaecher D , DeBakey ME , Lawrie GM , Morrisett JD . Quantitation and localization of apolipoproteins(a) and B in coronary artery bypass vein grafts resected at re-operation. Arteriosclerosis 1989;9:593–603.

Scanu AM . Lipoprotein(a): a potential bridge between the fields of atherosclerosis and thrombosis. Arch Pathol Lab Med, 1988;112:1045–47.

Scanu AM , Fless GM . Lipoprotein(a): heterogeneity and biological relevance. J Clin Invest 1990;85:1709–15.

Boonmark NW , Lou XJ , Yang ZJ , Schwartz K Zhang JL , Rubin EM , Lawn RM . Modification of apolipoprotein(a) lysine binding site reduces atherosclerosis in transgenic mice. J Clin Invest 1997;100:558–64.

Hughes SD , Lou XJ , Ighani S , Verstuyft J , Grainger D , Lawn RM , Rubin EM . Lipoprotein(a) vascular accumulation in mice. In vivo analysis of the role of Iysine binding sites using recombinant adenovirus. J Clin Invest 1997;100:1493–500.

Karmansky I , Shnader H , Palant A , Gruener N . Lysine-binding species of lipoprotein(a) in coronary artery disease. Eur J Clin Invest 1994;24:360–6.

Scanu AM , Pfaffinger D , Lee JC , Hinman J . A single point mutation (Trp72-Arg) in human apo(a) kringle 4-37 associated with a lysine binding defect in Lp(a). Biochim Biophys Acta, 1994;1227:41–5.

Scanu AM , Atzeni MM , Edelstein C , Tonolo G , Maioli M , Klezovitch O . Lipoprotein(a): identification of subjects with a superbinding capacity for fibrinogen. Clin Genet 1997;52:367–70.

Libby P , Egan D , Skarlatos S . Role of infectious agents in atherosclerosis and thrombosis. Circulation 1997;97:4095–101.