Restricted accessResearch articleFirst published online 2023-10
Long non-coding RNA RMST serves as a diagnostic biomarker in patients with carotid artery stenosis and predicts the occurrence of cerebral ischemic event: A retrospective study
The purpose of this retrospective study is to explore the diagnostic and prognostic roles of serum RMST in carotid artery stenosis (CAS).
Methods
Serum levels of RMST were detected in CAS patients, and the relationship between degree of carotid stenosis and RMST levels was analyzed. The ROC curve was drawn to evaluate RMST value in predicting the risk of CAS. Then, all CAS patients received a 5-year follow-up. K-M curve was used to analyze the significance of RMST on prognosis of CAS patients. Multi-factor cox logistic regression analysis was conducted to evaluate independent factors for outcome of CAS patients.
Results
An increased RMST expression was certified in CAS patients when compared with healthy controls. The increase of serum RMST expression was related to high degree of carotid stenosis. In addition, serum RMST was a possible diagnosis and an independent influencing factor of prognosis in patients with CAS.
Conclusions
Raised serum RMST level was found in patients with CAS. Detecting RMST expression levels was of high value for predicting the occurrence and outcomes in CAS.
ZhangKLinQZhangT, et al.Contemporary prevalence and risk factors of carotid artery stenosis in asymptomatic low-income Chinese individuals: a population-based study. Postgrad Med2020; 132: 650–656.
3.
ArasuRArasuAMullerJ. Carotid artery stenosis: an approach to its diagnosis and management. Aust J Gen Pract2021; 50: 821–825.
DharmakidariSBhattacharyaPChaturvediS. Carotid artery stenosis: medical therapy, surgery, and stenting. Curr Neurol Neurosci Rep2017; 17: 77.
8.
BeckmanJAAnselGMLydenSP, et al.Carotid artery stenting in asymptomatic carotid artery stenosis: JACC review topic of the week. J Am Coll Cardiol2020; 75: 648–656.
9.
AberAHowardAWoodsHB, et al.Impact of carotid artery stenosis on quality of life: a systematic review. Patient2019; 12: 213–222.
10.
BunchH. Gene regulation of mammalian long non-coding RNA. Mol Genet Genomics2018; 293: 1–15.
11.
ZhangXTangXLiuK, et al.Long Noncoding RNA Malat1 regulates cerebrovascular pathologies in ischemic stroke. J Neurosci2017; 37: 1797–1806.
12.
WangJRuanJZhuM, et al.Predictive value of long noncoding RNA ZFAS1 in patients with ischemic stroke. Clin Exp Hypertens2019; 41(7): 615–621.
13.
SimionVZhouHPierceJB, et al.LncRNA VINAS regulates atherosclerosis by modulating NF-κB and MAPK signaling. JCI Insight2020; 5(21): e140627.
14.
YinDXuFLuM, et al.Long non-coding RNA RMST promotes oxygen-glucose deprivation-induced injury in brain microvascular endothelial cells by regulating miR-204-5p/VCAM1 axis. Life Sci2021; 284: 119244.
15.
LiJWangNNieH, et al.Long non-coding RNA RMST worsens ischemic stroke via MicroRNA-221-3p/PIK3R1/TGF-β signaling pathway. Mol Neurobiol2022; 59: 2808–2821.
16.
Luzón-ToroBVillalba-BenitoLFernándezRM, et al.RMRP, RMST, FTX and IPW:novel potential long non-coding RNAs in medullary thyroid cancer. Orphanet J Rare Dis2021; 16: 4.
17.
WangLLiuDWuX, et al.Long non-coding RNA (LncRNA) RMST in triple-negative breast cancer (TNBC): expression analysis and biological roles research. J Cell Physiol2018; 233: 6603–6612.
18.
YangFLiuYHDongSY, et al.Co-expression networks revealed potential core lncRNAs in the triple-negative breast cancer. Gene2016; 591: 471–477.
19.
HuangLShumEYJonesSH, et al.A Upf3b-mutant mouse model with behavioral and neurogenesis defects. Mol Psychiatry2018; 23: 1773–1786.
20.
NgSYBoguGKSohBS, et al.The long noncoding RNA RMST interacts with SOX2 to regulate neurogenesis. Mol Cell2013; 51: 349–359.
21.
HouXXChengH. Long non-coding RNA RMST silencing protects against middle cerebral artery occlusion (MCAO)-induced ischemic stroke. Biochem Biophys Res Commun2018; 495: 2602–2608.
22.
PaciaroniMEliasziwMKappelleLJ, et al.Medical complications associated with carotid endarterectomy. North American Symptomatic Carotid Endarterectomy Trial (NASCET). Stroke1999; 30: 1759–1763.
23.
LalBKDuxMCSikdarS, et al.Asymptomatic carotid stenosis is associated with cognitive impairment. J Vasc Surg2017; 66: 1083–1092.
24.
WuSWuBLiuM, et al.Stroke in China: advances and challenges in epidemiology, prevention, and management. Lancet Neurol2019; 18: 394–405.
25.
BadeaRTerecoasaERibiganA, et al.Factors associated with severe carotid artery stenosis in a population with one of the highest incidences of ischemic stroke in Europe - single national center analysis. Maedica2020; 15: 339–347.
26.
BoursinPPaternotteSDercyB, et al.[Semantics, epidemiology and semiology of stroke]. Soins2018; 63: 24–27.
27.
CarreiraMDuarte-GamasLRocha-NevesJ, et al.Management of the carotid artery stenosis in asymptomatic patients. Rev Port Cir Cardiotorac Vasc2020; 27: 159–166.
28.
WangLJiaJHongZ, et al.Effects of chemerin and homocysteine levels and their associations with occurrence and development of ischemic cerebrovascular disease. Lipids Health Dis2021; 20: 108.
29.
ChengYHeW. Establishment of the intracranial hemodynamic model based on contrast medium and clinical applications. Medicine2016; 95: e5550.
30.
GaoJChenXShanC, et al.Autophagy in cardiovascular diseases: role of noncoding RNAs. Mol Ther Nucleic Acids2021; 23: 101–118.
31.
DolzSGórrizDTemblJI, et al.Circulating MicroRNAs as novel biomarkers of stenosis progression in asymptomatic carotid stenosis. Stroke2017; 48: 10–16.
32.
MaHDongA. Dysregulation of lncRNA SNHG1/miR-145 axis affects the biological function of human carotid artery smooth muscle cells as a mechanism of carotid artery restenosis. Exp Therap Med2021; 21: 423.
33.
QiaoPYanHWangJ. EGb761 protects brain microvascular endothelial cells against oxygen-glucose deprivation-induced injury through lncRNA Rmst/miR-150 axis. Neurochem Res2020; 45: 2398–2408.
34.
GuJXZhangXMiaoRC, et al.Six-long non-coding RNA signature predicts recurrence-free survival in hepatocellular carcinoma. World J Gastroenterol2019; 25: 220–232.
35.
ChenCZhangSWeiY, et al.LncRNA RMST regulates neuronal apoptosis and inflammatory response via sponging miR-150-5p in Parkinson's Disease. Neuroimmunomodulation2021; 29: 55–62.
