177 Lu-DOTATATE Peptide Receptor Radionuclide Therapy: Indigenously Developed Freeze Dried Cold Kit and Biological Response in In-Vitro and In-Vivo Models

Abstract

Somatostatin receptors (SStR) based ¹⁷⁷Lu-DOTATATE therapy is known as one of the highly effective neuroendocrine tumors (NETs) treatment strategy. Development of DOTATATE freeze-dried kit for imaging and therapy of SStR positive NETs is a prime goal in neuroendocrine cancer research. The present work describes the development of ¹⁷⁷Lu-DOTATATE freeze dried cold kit for indigenous needs, through technology development fund (TDF) program offered by Higher Education Commission (HEC) Pakistan. The parameters for freeze dried kit production was optimized and tested the stored lyophilized cold kits for different time intervals after labeling with ¹⁷⁷Lu radioisotope. The effect of ligand to radionuclide ratio, pH and reaction time at 90°C was recorded. Five times greater molar concentration of ligand, pH 5 and 30 min reaction time were the effective reaction conditions for maximum radiochemical yield. The radiolabeling yield at 1 day, 1-week and 4-week post storing period showed ∼100% radiochemical yield. The biodistribution study using rat model depicted the absence of non-targeted accumulation while glomerular filtration rate also explains the rapid renal washout. Cytotoxicity study showed quite favorable results for subjecting the radiopharmaceutical to clinical practice in Pakistan.

Keywords

neuroendocrine tumors NET SStR radiopharmaceuticals

Introduction

Molecular imaging technique (MIT) is well known to oncology setup due to its efficiency in imaging, staging and fixing the malignant diseases. MITs such as single photon emission computed tomography (SPECT) and positron emission tomography (PET) are frequently practiced at oncology centers using target specific radiolabeled biological molecules for diagnosis of deep-seated infections and malignancies.¹ Gamma or positron emitter radionuclide such as technetium-99m (^99mTc), indium-111 (¹¹¹In), gallium-68 (⁶⁸ Ga) and fluorine-18 (¹⁸F) labeled biological molecules are used for imaging process,^2

-5 while beta or alpha emitter radionuclide such as leutitium-177 (¹⁷⁷Lu), yttrium-90 (⁹⁰Y), rhenium-188 (¹⁸⁸Re), actinium-225 (²²⁵Ac) are used for therapeutic procedures.^6

-9 A variety of radiolabeled regulatory peptides are in clinical trials and few are in clinical practice for imaging and therapy of NETs. NET imaging and therapy bases on peptide-receptor affinity.

NETs form a heterogeneous group of neoplasms that have ability to grow at any place of human body with the secretion of peptides and neuroamines. The gastroenteropancreatic endocrine tumors are the most common (∼67.5%) among NETs including pancreas carcinoma (30%-40%), the small-intestine (15%-20%) and the rectum cancers (5%-15%) followed by bronchopulmonary tumors (25.3%).^10
-12 An exclusive feature of these cancers/tumors is the overexpression of SStR which act as NET markers and the principle targets of radionuclide-labeled somatostatin peptide analogues for therapy.¹³ Somatostatin is a naturally occurring hormone commonly known as growth hormone-inhibiting factor. It is cyclic peptide hormone bearing disulphide cyclic linkage with either 14 (SS-14) or 28 amino-acids (SS-28).¹⁴ The former form predominant in brain while latter sequence produced by intestinal enteroendocrine cells. These receptors also express in the adrenals, and the pancreas. It mainly regulates the endocrine system via interaction with G-protein coupled SStR which mainly comprises of 5 subtypes (SStR1-5).¹⁵ There are many neuroendocrine origin tissues that show overexpression of SStR in its malignancy state. NETs, predominantly characterized by the overexpression of any 1 of the 5 subtypes of SStR. Molecular imaging and therapy, based on targeting the SStR is justified with the coherence between the structure of the somatostatin analogue and SStR subtype.^16,17 Highly attractive somatostatin analogue that target SStR2 with its maximum efficiency (∼100%) is [DOTA0, Tyr3, Thr8]-octreotide (DOTATATE).¹⁸ SStR2 is overexpressed in a majority of NETs, including small-cell lung carcinomas (SCLCs), breast tumor tissues, and pancreatic cancer.^18
-20 DOTATATE is being practiced clinically, either for imaging NETs by labeling with ¹¹¹In or ⁶⁸ Ga, or for therapy by labeling with ¹⁷⁷Lu. This particular type of therpy which base on radiolabeled peptide and receptors interaction is termed as peptide receptor radionuclide therapy (PRRT). The procedure is gaining ample attention for its ∼100% efficiency to fix NETs, which on contrary are hard to treat either through surgery or chemotherapy. PRRT, however fix the malignancy at molecular level that is acomplished by using radionuclide labeled somatostatin peptide analogues which transfer therapeutic dose of betta or alpha radiations to cancer cells.²¹

¹⁷⁷Lu labeled DOTATATE has shown promising results in phase-3 clinical trials which sufficiently satisfy the requirements for FDA approval. Recently, Spain has approved the ¹⁷⁷Lu-DOTATATE therapy on the bases of its well tolerable efficiency in treating SStR2 positive NETs.¹³ Currently Europe USA and few other developed countries offer ¹⁷⁷Lu-DOTATATE therapy at tolerable cost for thier peoples. In Pakistan the ¹⁷⁷Lu-DOTATATE therapy is also practicing in few oncology centers by importing freeze dried cold kit at very high cost-per-patient. The cost-per-patient could be reduced at lowest possible level by indigenous development of ¹⁷⁷Lu-DOTATATE freeze dried cold kit and production of ¹⁷⁷LuCl₃ at high specific activity level. HEC-Islamabad through TDF scheme provided the opportunity to develop ¹⁷⁷Lu-DOTATATE freeze dried cold kit to fulfill the indigenous need at affordable cost per patient. This manuscript is a show-case of partial pre-clinical results to share effectiveness of indigenously developed ¹⁷⁷Lu-DOTATATE cold kit and providing the ground for further clinical tests and to initiate the process for regional approval for clinical practice in country.

Material and Methods

Materials

All the chemicals were of analytical grade and purchased from Sigma-Aldrich, Fisher Scientific and Alfa Aesar. Whatman 3 chromatographic paper was purchased from Agilent

Germany. [DOTA⁰, Tyr³, Thr⁸]-octreotide were synthesized in GL-Biochem laboratory, China under sterile conditions. The ¹⁷⁷Lu radionuclide in the form of ¹⁷⁷LuCl₃ was obtained from Pakistan Atomic Research Reactor-1 (PARR-1), PINSTECH, Islamabad with sufficiently good specific activity required for research purposes and animal study.

Formulation of Freeze Dried DOTATATE kit

In order to formulate freeze dried cold kit of DOTATATE, the radiochemical preparation was assessed at different reaction conditions and test the effect of ligand-radioactivity ratio, pH and reaction time to obtain maximum labeling yield. The optimized reaction conditions at which ∼100% labeling yield of ¹⁷⁷Lu-DOTATATE was obtained was used to prepare freeze dried kits. Brief description of freeze-dried DOTA-TATE kit preparation is summarized as follow; DOTATATE acetate was dissolved in ultrapure filtered water to prepare the stock solution having concentration 1µg/ µL and stored in 50 µL aliquots. A solution of gentisic acid was prepared by dissolving 40 mg of gentisic acid in 2 mL of 0.4 M acetate buffer in dark. Stock solution of ascorbic acid was prepared by dissolving 210 mg of ascorbic acid in 2 mL 0.4 M acetate buffer having pH 4.8 and stored the solution in dark. 50 µg of DOTATATE (1µg/ µL in water) was taken in to an amber vial and added 5 mg of gentisic acid along with 26 mg of ascorbic acid from stock solution. The resulting solution was mixed thoroughly and pH was adjusted to 5. The solution was passed through 0 .22 µm millipore filter and lyophilized for 24 h. The kits were stored at 4°C in refrigerator.

Production of ¹⁷⁷LuCl₃

Production and radiochemical processing of ¹⁷⁷Lu in the form of ¹⁷⁷LuCl₃ salt was carried out at Pakistan Atomic Research Reactor-1(PARR-1), PINSTECH, Islamabad, Pakistan. The ¹⁷⁷LuCl₃ obtained from PARR-1 was used for preparation and optimization of cold kit parameters. Typically, 100 µg of isotopically enriched Lu₂O₃ target (52% in ¹⁷⁶Lu) was dissolved in concentrated HCl to get LuCl₃. The resulting solution was then dried in a quartz tube in desiccators and reconstituted with ultrapure water—dried it again, sealed the tube in aluminum sheet for irradiation. The sample was irradiated at a thermal neutron flux of 1.5 × 10¹⁴ ncm⁻² s⁻¹ for a period of 18 h. The irradiated target was allowed to cool for 12 h and dissolved in gently warm HCl solution (pH 3-4) followed by cooling to room temperature and filtering through 0.22 µm millipore filter paper to obtain pure ¹⁷⁷LuCl₃. Thus obtained ¹⁷⁷LuCl₃ could be used for therapeutic dose of ¹⁷⁷Lu-DOTATATE.

Labeling of DOTATATE Kit With ¹⁷⁷Lu

The freeze dried cold kit of DOATATAE was reconstituted with sterile water and diluted to pH 5 followed by the addition of ¹⁷⁷LuCl₃ solution. The mixture was heated to 90°C for 35 min to label the DOTATATE with ¹⁷⁷Lu. Different labeling reaction was performed by increasing DOTATATE concentration such as 1,2,3,4, or 5 times as compared to ¹⁷⁷Lu.

Quality Control Studies

Following the preparation of ¹⁷⁷Lu-DOTATATE and cooling at room temperature, the radiochemical mixture was subjected for quality control analysis using chromatography process.

Paper Chromatography Analysis

The radiochemical purity of ¹⁷⁷Lu-DOTATATE was determined by paper chromatography. The analysis was carried out by spotting an aliquot of 1 µL reaction mixture at 1.5 cm from one end of the 14 cm long chromatography paper strip (Whatman 3). The strip was developed using 50% acetonitrile aqueous solution. After drying the strip, it was scanned through Radio-TLC scanner having flat type NaI(Tl) detector. The R _f value was calculated through software B-SCAN.

Stability of ¹⁷⁷Lu-DOTATATE Freeze Dried Kits

The radiochemical yield of ¹⁷⁷Lu-DOTATATE which reconstituted using freeze dried cold kits which have been stored for 24 h, 1 week and 4 weeks, was investigated by incubating the radiopharmaceutical at room temperature for predefined time intervals through paper chromatography. The percent yield after radiolabeling reaction revealed that DOTATATE analogue remained stable in one month storing period.

Biodistribution Studies

Biodistribution pattern of ¹⁷⁷Lu labeled FDK of DOTATATE was studied in normal wistar rats each weighing 40-50 g in a group of 3-5. The ¹⁷⁷Lu-DOTATATE solution was further diluted with saline to 25 MBq activity/mL. An aliquot of 200 µL of ¹⁷⁷Lu-DOTATATE solution was then administration in animals through the tail vein of rats. For each time point, 3 animals were injected. The animals were given chloroform anesthesia prior to sacrifice at 2 h, 24 h, 48 h, and 72 h postinjection. Immediately, after animal sacrifice, blood was collected through cardiac puncture and counted the radioactivity using well-type NaI(Tl) scintillation counter. Different organs of the sacrificed animals were also excised, saline-washed, dried over filter paper, weighed and counted the radioactivity. The percentage of injected activity (%IA) accumulated in various organs was calculated. The activity excreted was indirectly determined from the difference between total injected activity (IA) and %IA accounted for all the organs.

Glomerular Filtration Rate

Glomerular filtration rate (GFR) study was performed using protocol as described previously published reports.²² Briefly, an aliquot of 200 µL of ¹⁷⁷Lu-DOTATATE was administered into the ear vein of a group of 3 New Zealand white rabbits (kept under starvation conditions) early morning on the day of experiment. The GFR was analyzed with built-in software in SPECT camera software, and the urine activity was also calculated simultaneously with GFR counting.

Hematology, Cytotoxicity and Histopathology Studies

Cytotoxicity of ¹⁷⁷Lu-DOTATATE against the normal biological tissues was evaluated by injecting the labeled peptide to the New Zealand white rabbit each weighing about 2-3 Kg. The cytotoxicity in animals were studied in following 3 groups; i) using over-dose of the ¹⁷⁷Lu-DOTATATE in which 555 MBq was injected and sacrificed after 3 weeks (21 days) postinjection period, ii) using therapeutic dose of ¹⁷⁷Lu-DOTATATE according to weight of the rabbit and completed the 3 doses each after 1 month, and iii) injecting maximum dose of the cold DOTATATE. The pre-injection (Pr-I) and post-injection (Ps-I) rabbits’ blood samples were collected for biochemical and hematology studies to analyze the systemic effect on blood parameters in all 3 conditions. All the animals were sacrificed and specific vital organs were excised. The tissues of heart, kidney and Lungs were taken and fixed with neutral formalin (10%), embedded in paraffin, and then manually sectioned with a microtome to obtain 4–5 mm-thick paraffin sections. Dewaxed sections were then stained with hematoxylin and eosin (H&E) method and observed under the light microscope for cytotoxicity and histopathology results.

Results

Quality Control Parameter Study and Radiochemical Yield

The radiochemical yield greatly affected by ligand to radionuclide ratio, pH and reaction time. In order to develop freeze dried DOTATATE kit and to achieve maximum radiochemical yield, and consequently maximum therapeutic advantages; the radiolabeling of ¹⁷⁷Lu with DOTATATE were studied in detail using different reaction conditions such as ligand to radionuclide ratio in which ligand concentration was increased from 1 to 5 times as compared to ¹⁷⁷Lu (Figure 1), pH in the range of 3.5 to 6.5 units (Figure 2) and 10 to 50 min reaction time (Figure 3) at 90°C reaction temperature. At each set of reaction conditions the reaction mixture was tested for radiochemical yield using paper chromatography. At optimized reaction conditions, the radiochromatogram of reaction mixture was developed in 2 mobile systems as shown in Figure 4.

Figure 1.

Effect of ligand concentration against fixed radioactivity of ¹⁷⁷LuCl.

Figure 2.

Effect of pH on radiochemical yield.

Figure 3.

Effect of on reaction time on radiochemical yield.

Figure 4.

Radiochromatogram of ¹⁷⁷Lu-DOTATATE quality control developed in 2 different.

Stability of DOTATATE Freeze Dried Kit

Shelf life stability of DOTATATE freeze-dried kit was assessed after 24 h, 1 week and 4 weeks of its preparation by labeling with ¹⁷⁷Lu. The results of radiochemical yield of labeled freeze dried kits are shown in Figure 5.

Figure 5.

Freeze dried kit stability study by labeling with ¹⁷⁷Lu.

Biodistribution Study

Biodistribution of ¹⁷⁷Lu-DOTATATE was assessed in wister rates. Briefly, at 2, 24, 48 and 72 h post injection of 200 µL of an aliquot of ¹⁷⁷Lu-DOTATATE through tail vein, the rats were anesthetized, sacrificed, organ excised, washed and subjected for radioactivity counts. The data were analyzed and summarized in the form of graph as shown in Figure 6.

Figure 6.

Biodistribution pattern of ¹⁷⁷Lu-DOTATATE in normal wister rats’ models.

GFR Study

GFR study was conducted to test the effect of indigenously developed ¹⁷⁷Lu-DOTATATE kit on renal function. Renal filtration rate was noted at regular intervals that was analyzed through built in GFR software in SPECT camera. The results of GFR study are shown in Figure 7.

Figure 7.

Glomerular filtration rate study to evaluate renal functioning and renal accumulation behavior of ¹⁷⁷Lu-DOTATATE.

Hematology, Cytotoxicity and Histopathology Study

Table 1 shows the hematology results of ¹⁷⁷Lu-DOTATATE administrated animal model. Due to the radionuclide involvement in cancer therapeutic procedures, it is more likely that the disturbance of blood parameters by ionizing radiations may take place. Further, the ionizing radiations can also cause cytotoxicity in animal body. The results of cytotoxicity study using histopathology are shown in Table 1 and Table 2; while the effect on tissues of different key organs are shown in Figure 8.

Table 1.

Blood Parameter Study to Evaluate Cytotoxicity of ¹⁷⁷Lu-DOTATATE and Free ¹⁷⁷LuCl3.

Sr. No.	Parameter	¹⁷⁷Lu-DOTATATE (20 µg)		¹⁷⁷LuCl₃		Normal range
Sr. No.	Parameter	Pr-I	Ps-I	Pr-I	Ps-I	Normal range
1.	WBC /µl	4800	4700	8600	8400	4000-11000
2.	RBC m/µl	4.0	3.89	5.47	6.22	4.5-6.0
3.	Hemoglobin g/dl	11.6	11.4	12.9	12.3
4.	Hematocrit %	30.0	32.3	35	36.4	40-50
5.	MCV fl	51	52.8	63.9	58.6	76-96
6.	MCH pg	21	19.4	23.6	19.8	27-31
7.	MCHC g/dl	32	31	36.9	32.3	32-36
8.	Platelets/µl	490000	489000	311000	545000	140000-450000
	Differentia Count
9.	Neutrophils%	70	75	62	76	50-75
10.	Lymphocytes%	25	20	31	16	20-45
11.	Monocytes%	1	3	4	5	02-08
12.	Eosinophils%	1	2	3	3	0-6

Table 2.

Liver and Kidney Function Parameters Study.

Sr. No.	Liver & kidney function parameters	¹⁷⁷Lu-DOTATATE		Free ¹⁷⁷LuCl₃		Normal range
Sr. No.	Liver & kidney function parameters	Pr-I	Ps-I	Pr-I	Ps-I	Normal range
1.	Serum Bilirubin (total) (mg/dl)	0.5	0.7	0.5	0.7	∼1.0
2.	Serum ALT(SGPT) (µ/L)	39	68	80	84	∼46
3.	Serum AST(SGOT)(µ/L)	22	82	30	71	∼35
4.	Serum ALP (µ/L)	134	111	98	114	Adults <258 children <600
5.	Serum uric acid (mg/dl)	3.2	4.2	5.1	3.3	∼3.5-7
6.	Serum urea (µ/L)	42	3.7	38	32	∼10-50
7.	Serum creatinine (µ/L)	1.1	0.8	1.1	0.7	∼0.6-1.2

Figure 8.

Histopathology images of control, free ¹⁷⁷Lu and ¹⁷⁷Lu-DOTATATE administrated kidney, liver and heart tissues.

Discussion

Currently, ¹⁷⁷Lu-DOTATATE is gaining breathtaking attention in therapy of NETs followed by ⁶⁸Ga-DOTATATE mediated PET imaging for initial diagnosis, and selection of patients for PRRT.²³ The agent is under different clinical trials to get approval from respective authorities in different regions of world.^24
-26 In Pakistan, we have also developed the freeze-dried kit of DOTATATE for imaging and therapy of NETs at affordable cost.

The quality control analysis for developing freeze-dried kit at different ligand to radionuclide ratio, pH and reaction time indicate 5 times higher ligand ratio as compared to radionuclide facilitate 100% labeling yield at pH 5 and 30 min reaction time at 90°C temperature. Less than this value increases the free radioactivity. Varying the pH and reaction time directly affect the labeling yield. At optimized reaction conditions 100% labeling yield guarantied the promising efficacy of therapeutic procedure. The freeze-dried kit was tested at 24 h, 1-week and 4-weeks of post-preparation/storing period. The results indicate consistency in radiochemical purity and stability of the kit which are the primary requirements for freeze dried kit to provide at remote areas or to store for number of week to utilize on demand for therapeutic procedures. The ¹⁷⁷Lu-labeling with freeze dried kit in intervals of weeks, analyzed using chromatography, the results show the promising ability to bind with ¹⁷⁷Lu with maximum labeling yield.

The ¹⁷⁷Lu-DOTATATE kit was subjected to biodistribution in animal model to record the accumulated activity in different body organs. All organs showed normal uptake which gradually washed-out from organ tissue. The normal uptake phenomenon of ¹⁷⁷Lu-DOTATATE is mainly due to its selectivity for target which indicated the efficacy of freeze dried kit as well. Kidneys, however showed slightly more accumulation of ¹⁷⁷Lu-DOTATATE and the blood creatinine level remain in the limit. Previously, reported data showed slight increase (11.1%) of blood creatinine level along with renal accumulation.²⁴ The whole biodistribution pattern, however not showed unusual accumulation which expresses that there were no overexpressed SStR cells/tissues.^27,28 The GFR results showed the peak time 8.1 and 9.8 min, percentage uptake 56.5% and 67.23% and GFR value 34.67 mL/min was calculated. The excretion rate of ¹⁷⁷Lu-DOTATATE indicated the normal renal filtration which indicate thevalidity of the kit for clinical study.²⁹ The GFR value of gold standard GFR agent (^99mTc-DTPA) is 72 mL/min which is small organic molecule and non-proteinous in nature, but due to protein nature of DOTATATE the renal filtration was recorded slow.^7,22 The radiolabeled peptide therapy, however face the renal accumulation in general such as in case of imaging and therapy using radiolabeled minigastrin peptides²⁹ but in contrast to other radiolabeled peptides ¹⁷⁷Lu-DOTATATE shows rapid renal filtration which is the success point of ¹⁷⁷Lu-DOTATATE therapy.³⁰

Cytotoxicity study of ¹⁷⁷Lu-DOTATATE kits studied after 21st day of its preparation, by collecting the blood of ¹⁷⁷Lu-DOTATATE administrated animal at Pr-I and Ps-I time points. The complete blood profile results indicated non-significant difference between Pr-I and Ps-I blood analysis. The minute difference in blood parameters is mainly due to ionizing potential of β-radiations, however it could be tolerating. The histopathology, study also showed the physiological changes as exist in hematology study of blood. The cytotoxicity was further analyzed by studying liver and kidney functioning parameters. The values of serum bilirubin remained within normal limit in Pr-I and Ps-I ¹⁷⁷Lu-DOTATATE and free ¹⁷⁷LuCl₃ administration. Other parameters; serum ALP, serum uric acid, serum urea and serum creatinine remained in-range after administrating the ¹⁷⁷Lu-DOTATATE and free ¹⁷⁷LuCl_3, while serum ALT (SGPT) and serum AST (SGOT) increased in either case of administration, ¹⁷⁷Lu-DOTATATE or free ¹⁷⁷LuCl₃. Serum ALT(SGPT) was increased to 68 μ/L in case of ¹⁷⁷Lu-DOTATATE Ps-I and 84 μ/L in case of free ¹⁷⁷LuCl₃ Ps-I analysis. The Serum ALT(SGPT) limit in blood is ∼46. The other liver parameter, serum AST(SGOT) also showed increase in its level i.e. 82 μ/L in case of 177Lu-DOTATATE Ps-I and 71 μ/L in case of free 177LuCl3 Ps-I as compared to its higher limit, ∼35 μ/L. The increase in both liver markers indicate minor liver damage. It can be considered that some damaging effect may possible due to β-radiations. Moreover, the liver was the most common site of NET metastasis (91.7%) which also indicate the presence of SStR which facilitate the accumulation of DOTATATE and hence the damaging.¹³ The injection of free ¹⁷⁷Lu dose equivalent to the ¹⁷⁷LuDOTATATE dose, however showed comparable values. The histopathology analysis showed non-significant alteration in tissue morphology except some changes were seen in liver slides.

Conclusion

The radiochemical yield, stability study of freeze-dried kit, biodistribution, GFR values, and cytotoxicity results showed good development, however it needs further improvement to make the kit of world health standards and to submit the experimental data to “Animal and Human Ethics Board” of institute for further guidelines and experimentation to conduct Phase-I & II trials.

Footnotes

Authors’ Note

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Acknowledgments

We greatly acknowledge the efforts of HEC in developing research culture in Pakistan and also portfolio of technologies which are being practiced in developing countries, to facilitate the public of Pakistan. We thank HEC for awarding Technology Development Fund (TDF) No. TDF-001, to develop ¹⁷⁷Lu-DOTATATE therapeutic technology in Pakistan. We also acknowledge the efforts of our respected colleagues to facilitate and guided where we needed, especially Dr. Ammad Hussain Qureshi, DG PINSTECH, Dr. Nasir Majeed Mirza, Rector, PIEAS, Dr. Faheem Ahmad, Director NORI, Dr. Shazia Fatima, DCMO, Dr. Zain khurshid, SMO, Mr. Naseer Ahmed, Senior pharmacist. NORI, Prof. Dr Saeed Alam (histopathologists) IMDC, Islamabad, Dr. Shabana Saeed, DCMO, PIEAS, Dr. Rashid Rasheed (INOR, Abbottabad), Dr. Hussain Ali NIH, Dr. Saima Tariq (PS), Mr Javaid Iqbal, Mr. Kashif Adnan, Mr. Ijaz Ahmad, Technologist. We are also thankful to Director ORIC PIEAS.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

ORCID iD

Syed Ali Raza Naqvi

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177 Lu-DOTATATE Peptide Receptor Radionuclide Therapy: Indigenously Developed Freeze Dried Cold Kit and Biological Response in In-Vitro and In-Vivo Models

Abstract

Keywords

Introduction

Material and Methods

Materials

Formulation of Freeze Dried DOTATATE kit

Production of 177LuCl3

Labeling of DOTATATE Kit With 177Lu

Quality Control Studies

Paper Chromatography Analysis

Stability of 177Lu-DOTATATE Freeze Dried Kits

Biodistribution Studies

Glomerular Filtration Rate

Hematology, Cytotoxicity and Histopathology Studies

Results

Quality Control Parameter Study and Radiochemical Yield

Stability of DOTATATE Freeze Dried Kit

Biodistribution Study

GFR Study

Hematology, Cytotoxicity and Histopathology Study

Discussion

Conclusion

Footnotes

Authors’ Note

Acknowledgments

Declaration of Conflicting Interests

Funding

ORCID iD

References

Production of ¹⁷⁷LuCl₃

Labeling of DOTATATE Kit With ¹⁷⁷Lu

Stability of ¹⁷⁷Lu-DOTATATE Freeze Dried Kits