Putative Inhibitory Extracellular Matrix Molecules Do Not Prevent Dorsal Root Regeneration into Fetal Spinal Cord Transplants

Abstract

We examined the distribution of several extracellular matrix molecules (ECM) and their relationship to regenerating axons in embryonic day 14 spinal cord transplants 1 to 12 weeks after transplantation into adult rats. We used immunocytochemical tech niques to label chondroitin sulfate proteoglycans (CSPGs) and tenascin-C in adjacent sections. Synthesis of these molecules by astrocytes is thought to be one mechanism by which astrocytes inhibit regeneration in the central nervous system (CNS); glial fibrillary acidic protein antibody was used to label astrocytes and examine their rela tionship to both the ECM molecules and regenerating calcitonin gene-related pep tide (CORP)-contammg dorsal roots. We also compared the expression and distribu tion of these five markers in transplants with normal spinal cord development.