HUGO ( FNDC3A ): a New Gene Overexpressed in Human Odontoblasts

Abstract

Previously, we established a subtractive cDNA library enriched in odontoblast-specific genes and hypothesized that new, previously unidentified, markers would be present, associated with the odontoblast phenotype. In this paper, we report the first characterization of a new gene we have named HUGO, and its associated deduced protein sequence. This gene expression is under the control of two alternative promoters, resulting in the synthesis of two proteins, one of which, HUGO2, is included in the other, HUGO1. HUGO proteins are mainly composed of a proline-rich region at the N-terminus, 8 type III-fibronectin modules, and a transmembranous helix at the C-terminus. In odontoblasts, the proteins are located in Golgi vesicles. However, they display a broader expression pattern, since they are also expressed by nerve fibers in the dental pulp and other tissues (e.g., trachea, brain, kidney), as demonstrated by immunohistochemistry and qPCR, respectively. Their location in odontoblasts suggests a role in collagen and glycosaminoglycan synthesis.

Get full access to this article

View all access options for this article.

References

Allard B, Magloire H, Couble ML, Maurin JC, Bleicher F -2006-. Voltage-gated sodium channels confer excitability to human odontoblasts: possible role in tooth pain transmission. J Biol Chem 281:29002–29010.

Bleicher F, Couble ML, Farges JC, Couble P, Magloire H -1999-. Sequential expression of matrix protein genes in developing rat teeth. Matrix Biol 18:133–143.

Buchaille R, Couble ML, Magloire H, Bleicher F -2000a-. A subtractive PCR-based cDNA library from human odontoblast cells: identification of novel genes expressed in tooth forming cells. Matrix Biol 19:421–430.

Buchaille R, Couble ML, Magloire H, Bleicher F -2000b-. Expression of the small leucine-rich proteoglycan osteoadherin/osteomodulin in human dental pulp and developing rat teeth. Bone 27:265–270.

Corpet F -1988-. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16:10881–10890.

Couble ML, Farges JC, Bleicher F, Perrat-Mabillon B, Boudeulle M, Magloire H -2000-. Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int 66:129–138.

Durand SH, Flacher V, Romeas A, Carrouel F, Colomb E, Vincent C, et al. -2006-. Lipoteichoic acid increases TLR and functional chemokine expression while reducing dentin formation in in vitro differentiated human odontoblasts. J Immunol 176:2880–2887.

Goldberg M, Smith AJ -2004-. Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering. Crit Rev Oral Biol Med 15:13–27.

Jernvall J, Thesleff I -2000-. Reiterative signaling and patterning during mammalian tooth morphogenesis. Mech Dev 92:19–29.

10.

Kuo MY, Lan WH, Lin SK, Tsai KS, Hahn LJ -1992-. Collagen gene expression in human dental pulp cell cultures. Arch Oral Biol 37:945–952.

11.

Linde A, Lundgren T -1995-. From serum to the mineral phase. The role of the odontoblast in calcium transport and mineral formation. Int J Dev Biol 39:213–222.

12.

Magloire H, Lesage F, Couble ML, Lazdunski M, Bleicher F -2003-. Expression and localization of TREK-1 K+ channels in human odontoblasts. J Dent Res 82:542–545.

13.

Mesgouez C, Oboeuf M, Mauro N, Colon P, MacDougall M, Machtou P, et al. -2006-. Ultrastructural and immunocytochemical characterization of immortalized odontoblast MO6–G3. Int Endod J 39:453–463.

14.

Meshorer E, Toiber D, Zurel D, Sahly I, Dori A, Cagnano E, et al. -2004-. Combinatorial complexity of 5′ alternative acetylcholinesterase transcripts and protein products. J Biol Chem 279:29740–29751.

15.

Pankov R, Yamada KM -2002-. Fibronectin at a glance. J Cell Sci 115:3861–3863.

16.

Ruch JV, Lesot H, Bègue-Kirn C -1995-. Odontoblast differentiation. Int J Dev Biol 39:51–68.

17.

Stanislawski L, Carreau JP, Pouchelet M, Chen ZH, Goldberg M -1997-. In vitro culture of human dental pulp cells: some aspects of cells emerging early from the explant. Clin Oral Investig 1:131–140.

18.

Takagi M, Parmley RT, Denys FR -1981-. Ultrastructural localization of complex carbohydrates in odontoblasts, predentin, and dentin. J Histochem Cytochem 29:747–758.

19.

Veron MH, Couble ML, Caillot G, Hartmann DJ, Magloire H -1990-. Expression of fibronectin and type I collagen by human dental pulp cells and gingiva fibroblasts grown on fibronectin substrate. Arch Oral Biol 35:565–569.

20.

Veron MH, Couble ML, Magloire H -1993-. Selective inhibition of collagen synthesis by fluoride in human pulp fibroblasts in vitro. Calcif Tissue Int 53:38–44.

21.

Weinstock M, Leblond CP -1974-. Synthesis, migration, and release of precursor collagen by odontoblasts as visualized by radioautography after -3H-proline administration. J Cell Biol 60:92–127.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.53 MB

0.00 MB