Effects of Hydrogen Peroxide on Human Dentin Structure

Abstract

It has been hypothesized that hydrogen peroxide (H₂O₂) bleaching may cause destruction of dentin by a mechanism of protein oxidation. However, to our knowledge, there has been no direct chemical evidence to validate this viewpoint. To investigate the effects of H₂O₂ on the structure of human dentin, we used Fourier transform infrared spectroscopy (FTIR) and attenuated total reflection (ATR) spectroscopy. Human intact dentin specimens were treated either with 30% H₂O₂ or Hanks’ balanced salt solution (HBSS). Significant differences were observed in ATR spectra parameters. Additionally, demineralized dentin specimens were also tested. They were completely dissolved in the H₂O₂, but remained intact in the 0.1 N HCl and HBSS. The results suggested that H₂O₂ attacked both the organic and mineral components of dentin. Destruction of the organic components was mainly because of the oxidizing ability of H₂O₂, while changes in the mineral components were probably due to its acidity.

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