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Abstract

Osteoclasts play a vital role in orthodontic tooth movement. Transactivation of nuclear factor κB (NFκB) by phosphorylation of the p65 component of NFκB at amino acid 536 (p65*⁵³⁶) plays a role in osteoclast differentiation stimulated by receptor activator of nuclear factor κB-ligand (RANK-L). We hypothesized that this transactivation pathway might be involved in the responses of alveolar bone cells during orthodontic tooth movement. We detected sharp increases in the levels of p65*⁵³⁶ 3 and 12 hrs after the application of orthodontic stimuli in rats. In cell culture, osteoclast-like cells displayed no changes in p65*⁵³⁶ in response to RANK-L, but levels rapidly increased after the cells were mechanically scraped. We conclude that p65*⁵³⁶ is produced rapidly in response to orthodontic stimuli and mechanical insults, and may be important in bone remodeling associated with orthodontic tooth movement.

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Nuclear Factor κB p65 Phosphorylation in Orthodontic Tooth Movement

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