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Abstract

Post-eruptive loss of ameloblasts requires identification of alternative sources for these cells to realize tooth-tissue-engineering strategies. Recent reports showed that bone-marrow-derived cells can give rise to different types of epithelial cells, suggesting their potential to serve as a source for ameloblasts. To investigate this potential, we mixed c-Kit⁺-enriched bone marrow cells with embryonic dental epithelial cells and cultured them in re-association with dental mesenchyme. Non-dividing, polarized, and secretory ameloblast-like cells were achieved without cell fusion. Before basement membrane reconstitution, some bone marrow cells migrated to the mesenchyme, where they exhibited morphological, molecular, and functional characteristics of odontoblasts. These results show, for the first time, that bone-marrow-derived cells can be reprogrammed to give rise to ameloblast-like cells, offering novel possibilities for tooth-tissue engineering and the study of the simultaneous differentiation of one bone marrow cell subpopulation into cells of two different embryonic lineages.

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References

Borue X, Lee S, Grove J, Herzog EL, Harris R, Diflo T, et al. (2004). Bone marrow-derived cells contribute to epithelial engraftment during wound healing. Am J Pathol 165:1767–1772.

Butler WT, Bhown M, Brunn JC, D’Souza RN, Farach-Carson MC, Happonen RP, et al. (1992). Isolation, characterization and immunolocalization of a 53-kDal dentin sialoprotein (DSP). Matrix 12:343–351.

DenBesten PK, Machule D, Zhang Y, Yan Q, Li W (2005). Characterization of human primary enamel organ epithelial cells in vitro. Arch Oral Biol 50:689–694.

Fincham AG, Moradian-Oldak J, Simmer JP (1999). The structural biology of the developing dental enamel matrix. J Struct Biol 126:270–299.

George A, Sabsay B, Simonian PA, Veis A (1993). Characterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralization. J Biol Chem 268:12624–12630.

Gronthos S, Mankani M, Brahim J, Robey PG, Shi S (2000). Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA 97:13625–13630.

Grove JE, Bruscia E, Krause DS (2004). Plasticity of bone marrow-derived stem cells. Stem Cells 22:487–500.

Hirota S, Ito A, Morii E, Wanaka A, Tohyama M, Kitamura Y, et al. (1992). Localization of mRNA for c-kit receptor and its ligand in the brain of adult rats: an analysis using in situ hybridization histochemistry. Brain Res Mol Brain Res 15:47–54.

Hu B, Nadiri A, Bopp-Kuchler S, Perrin-Schmitt F, Lesot H (2005). Dental epithelial histomorphogenesis in vitro. J Dent Res 84:521–525.

10.

Hu B, Nadiri A, Bopp-Kuchler S, Perrin-Schmitt F, Peters, H, Lesot H (2006). Tissue engineering of tooth crown, root and periodontium. Tissue Eng (in press).

11.

Krause DS (2005). Engraftment of bone marrow-derived epithelial cells. Ann NY Acad Sci 1044:117–124.

12.

Krause DS, Theise ND, Collector MI, Henegariu O, Hwang S, Gardner R, et al. (2001). Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 105:369–377.

13.

MacDougall M, Zeichner-David M, Slavkin HC (1985). Production and characterization of antibodies against murine dentine phosphoprotein. Biochem J 232:493–500.

14.

Matthews W, Jordan CT, Wiegand GW, Pardoll D, Lemischka IR (1991). A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell 65:1143–1152.

15.

Mina M, Braut A (2004). New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes. Cells Tissues Organs 176:120–133.

16.

Ohazama A, Modino SA, Miletich I, Sharpe PT (2004). Stem-cell-based tissue engineering of murine teeth (see comments). J Dent Res 83:518–522. Comment in: J Dent Res 83:517.

17.

Osman M, Ruch JV (1980). Secretion of basal lamina by trypsin-isolated embryonic mouse molar epithelia cultured in vitro. Dev Biol 75:467–470.

18.

Robinson C, Kirkham J, Brookes SJ, Bonass WA, Shore RC (1995). The chemistry of enamel development. Int J Dev Biol 39:145–152.

19.

Ruch JV, Lesot H, Bègue-Kirn C (1995). Odontoblast differentiation. Int J Dev Biol 39:51–68.

20.

Simmer JP, Fincham AG (1995). Molecular mechanisms of dental enamel formation. Crit Rev Oral Biol Med 6:84–108.

21.

Smith AJ (2004). Tooth tissue engineering and regeneration—a translational vision! [comment] J Dent Res 83:517. Comment on: J Dent Res 83:518–522.

22.

Smith AJ, Lesot H (2001). Induction and regulation of crown dentinogenesis: embryonic events as a template for dental tissue repair? Crit Rev Oral Biol Med 12:425–437.

23.

Smith CE, Nanci A (1995). Overview of morphological changes in enamel organ cells associated with major events in amelogenesis. Int J Dev Biol 39:153–161.

24.

Spees JL, Olson SD, Ylostalo J, Lynch PJ, Smith J, Perry A, et al. (2003). Differentiation, cell fusion, and nuclear fusion during ex vivo repair of epithelium by human adult stem cells from bone marrow stroma. Proc Natl Acad Sci USA 100:2397–2402.

25.

Tran SD, Pillemer SR, Dutra A, Barrett AJ, Brownstein MJ, Key S, et al. (2003). Differentiation of human bone marrow-derived cells into buccal epithelial cells in vivo: a molecular analytical study. Lancet 361:1084–1088.

26.

Wang G, Bunnell BA, Painter RG, Quiniones BC, Tom S, Lanson NA Jr, et al. (2005). Adult stem cells from bone marrow stroma differentiate into airway epithelial cells: potential therapy for cystic fibrosis. Proc Natl Acad Sci USA 102:186–191.

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Bone Marrow Cells Can Give Rise to Ameloblast-like Cells

Abstract

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