Protein kinase C (PKC) is an important molecule involved in various cell function, and mediates induced secretion of vascular endothelial growth factor (VEGF). It is hypothesized that PKC and VEGF may be associated with tooth development. Using the laser microdissection method and real-time reverse-transcription-polymerase chain-reaction (RT-PCR), we investigated the expression of PKC βI and βII, VEGF, and amelogenin (used as a marker of differentiation to ameloblasts) in the inner and outer enamel epithelia, stellate reticulum, and dental papilla in each stage of the dental germ. We found that the expression levels of PKC βI and βII were increased in the inner enamel epithelium during the early bell stage. In addition, the increased expression levels of PKC βI and βII were accompanied by increased VEGF expression. These results indicate that PKC βI, βII, and VEGF are closely associated with the differentiation of the inner enamel epithelium to ameloblasts.
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