Resin Monomer 2-Hydroxyethyl Methacrylate (HEMA) is a Potent Inducer of Apoptotic Cell Death in Human and Mouse Cells

Abstract

Mechanisms by which the resin monomer 2-hydroxyethyl methacrylate (HEMA) induces hypersensitivity reactions in humans are not well-established, nor have the direct effects of HEMA on cell death been fully characterized. The objective of this study was to establish whether HEMA is capable of inducing apoptotic cell death, and whether differences exist in the levels of apoptotic death induced by HEMA in cells obtained from healthy individuals and from patients with established HEMA hypersensitivity. HEMA induced apoptotic death in Peripheral Blood Mononuclear Cells (PBMCs) obtained from both healthy and HEMA-sensitized patients and in the murine RAW cells in a dose-dependent manner. However, induction of cell death by HEMA was lower in PBMCs obtained from patients in comparison with healthy individuals. Studies reported in this paper demonstrate that HEMA induces apoptotic death, and that decreased susceptibility of lymphocytes to HEMA-mediated death might be an important mechanism for the generation and persistence of hypersensitivity reactions in patients.

Get full access to this article

View all access options for this article.

References

About I, Camps J, Mitsiadis TA, Bottero MJ, Butler W, Franquin JC -2002-. Influence of resinous monomers on the differentiation in vitro of human pulp cells into odotoblasts. J Biomed Mater Res 63:418–423.

Buckley DA, Du Vivier AW -2001-. The therapeutic use of topical contact sensitizers in benign dermatoses. Br J Dermatol 145:385–405.

Duncan CJ, Lawrie A, Blaylock MG, Douglas JG, Walsh GM -2003-. Reduced eosinophil apoptosis in induced sputum correlates with asthma severity. Eur Resp J 22:484–490.

Fischer B, Benzina S, Ganansia-Leymarie V, Denis JM, Bergerat JP, Dufour P, et al. -2004-. Cisplatin enhances the cytotoxicity of fast neutrons in a murine lymphoma cell line. Can J Physiol Pharmacol 82:140–145.

Fruhauf S, Zeller WJ -1991-. In vitro evaluation of platinum, titanium and ruthenium metal complexes in cisplatin-sensitive and -resistant rat ovarian tumors. Cancer Chemother Pharmacol 27:301–307.

Gerzina TM, Hume WR -1994-. Effect of dentine on release of TEGDMA from resin composite in vitro. J Oral Rehabil 21:463–468.

Gerzina TM, Hume WR -1995-. Effect of hydrostatic pressure on the diffusion of monomers through dentin in vitro. J Dent Res 74:369–373.

Gerzina TM, Hume WR -1996-. Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent 24:125–128.

Hamid A, Hume WR -1997a-. A study of component release from resin pit and fissure sealants in vitro. Dent Mater 13:98–102.

10.

Hamid A, Hume WR -1997b-. Diffusion of resin monomers through human carious dentin in vitro. Endod Dent Traumatol 13:1–5.

11.

Hamid A, Hume WR -1997c-. The effect of dentine thickness on diffusion of resin monomers in vitro. J Oral Rehabil 24:20–25.

12.

Jewett A, Giorgi JV, Bonavida B -1990-. Antibody-dependent cellular cytotoxicity against HIV-coated target cells by peripheral blood monocytes from HIV seropositive asymptomatic patients. J Immunol 145:4065–4071.

13.

Jewett A, Gan XH, Lebow LT, Bonavida B -1996-. Differential secretion of TNF-alpha and IFN-gamma by human peripheral blood-derived NK subsets and association with functional maturation. J Clin Immunol 16:46–54.

14.

Jewett A, Cavalcanti M, Bonavida B -1997-. Pivotal role of endogenous TNF-alpha in the induction of functional inactivation and apoptosis in NK cells. J Immunol 159:4815–4822.

15.

Jontell M, Hanks CT, Bratel J, Bergenholtz G -1995-. Effects of unpolymerized resin components on the function of accessory cells derived from the rat incisor pulp. J Dent Res 74:1162–1167.

16.

Koch S, Mayer F, Honecker F, Schittenhelm M, Bokemeyer C -2003-. Efficacy of cytotoxic agents used in the treatment of testicular germ cell tumours under normoxic and hypoxic conditions in vitro. Br J Cancer 89:2133–2139.

17.

Kogure T, Ueno Y, Iwasaki T, Shimosegawa T -2004-. The efficacy of the combination therapy of 5-fluorouracil, cisplatin and leucovorin for hepatocellular carcinoma and its predictable factors. Cancer Chemother Pharmacol 53:296–304.

18.

Kowalski ML, Grzegorczyk J, Pawliczak R, Kornatowski T, Wagrowska- Danilewicz M, Danilewicz M -2002-. Decreased apoptosis & distinct profile of infiltrating cells in the nasal polyps of patients with aspirin hypersensitivity. Allergy 57:493–500.

19.

Laflamme C, Israel-Assayag E, Cormier Y -2003-. Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Resp J 21:225–231.

20.

Li Y, Ellis KL, Ali S, El-Rayes BF, Nedeljkovic-Kurepa A, Kucuk O, et al. -2004-. Apoptosis-inducing effect of chemotherapeutic agents is potentiated by soy isoflavone genistein, a natural inhibitor of NF-kappaB in BxPC-3 pancreatic cancer cell line. Pancreas 28:e90–e95.

21.

Mori S, Murakami-Mori K, Jewett A, Nakamura S, Bonavida B -1996-. Resistance of AIDS-associated Kaposi’s sarcoma cells to Fas-mediated apoptosis. Cancer Res 56:1874–1879.

22.

Nelson BH, Martyak TP, Thompson LJ, Moon JJ, Wang T -2003-. Uncoupling of promitogenic and antiapoptotic functions of IL-2 by Smad-dependent TGF-beta signaling. J Immunol 170:5563–5570.

23.

Overbeeke R, Steffens-Nakken H, Vermes I, Reutelingsperger C, Haanen C -1998-. Early features of apoptosis detected by four different flow cytometry assays. Apoptosis 3:115–121.

24.

Sandberg E, Bergenholtz G, Eklund C, Dahlgren UI -2002-. HEMA bound to self-protein promotes auto-antibody production in mice. J Dent Res 81:633–636.

25.

Sarkar S, Kalia V, Montelaro RC -2003-. Caspase-mediated apoptosis and cell death of rhesus macaque CD4+ T-cells due to cryopreservation of peripheral blood mononuclear cells can be rescued by cytokine treatment after thawing. Cryobiology 47:44–58.

26.

Shen L, Rock KL -2004-. Cellular protein is the source of cross-priming antigen in vivo. Proc Natl Acad Sci USA 101:3035–3040.

27.

Wright SC, Jewett A, Mitsuyasu R, Bonavida B -1998-. Spontaneous cytotoxicity & tumor necrosis factor production by peripheral blood monocytes from AIDS patients. J Immunol 141:99–104.

Supplementary Material

Please find the following supplemental material available below.

For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.

For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.

0.00 MB

0.83 MB