Dentin is a useful model for the study of mineral maturation. Using Fourier Transform Infrared Imaging (FTIRI), we characterized distinct regions in developing dentin at 7-μm spatial resolution. Mineral-to-matrix ratio and crystallinity in bovine dentin from cervical and incisal parts of 3rd-trimester fetal compared with one-year-old incisor crowns showed that virtually all maturation stages in dentin could be spectroscopically isolated and analyzed. In the fetal incisors, mantle and circumpulpal dentin presented distinct patterns of mineral maturation. Gradients in both mineral properties examined were observed at the mineralization front and at the dentino-enamel junction.
Get full access to this article
View all access options for this article.
References
1.
Bhargava R, Levin IW -2001-. Fourier transform infrared imaging: theory and practice. Anal Chem73:5157–5167.
2.
Bodier-Houllé P, Steuer P, Meyer JM, Bigeard L, Cuisinier FJ -2000-. High resolution electron microscopic study of the relationship between human enamel and dentin crystals at the dentinoenamel junction. Cell Tissue Res301:389–395.
3.
Bonar LC, Roufosse AH, Sabine WK, Grynpas MD, Glimcher MJ -1983-. X-ray diffraction studies of the crystallinity of bone mineral in newly synthesized and density fractionated bone. Calcif Tissue Int35:202–209.
4.
Boskey AL, Paschalis EP, Binderman I, Doty SB -2002a-. BMP-6 accelerates both chondrogenesis and mineral maturation in differentiating chick limb-bud mesenchymal cell cultures. J Cell Biochem84:509–519.
5.
Boskey AL, Spevak L, Paschalis E, Doty SB, McKee MD -2002b-. Osteopontin deficiency increases mineral content and mineral crystallinity in mouse bone. Calcif Tissue Int71:145–154.
6.
Butler WT -1984-. Dentin collagen: chemical structure and role in mineralization. In: Dentin and dentinogenesis. Vol. II. Linde A, editor. Boca Raton, FL: CRC Press.
Camacho NP, West P, Torzilli PA, Mendelsohn R -2001-. FTIR microscopic imaging of collagen and proteoglycan in bovine cartilage. Biopolymers62:1–8.
9.
Gadaleta SJ, Camacho NP, Mendelsohn R, Boskey AL -1996-. Fourier transform infrared microscopy of calcified turkey leg tendon. Calcif Tissue Int58:17–23.
10.
Goldberg M, Septier D, Torres-Quintana M, Lecolle S, Hall R, Gafni G, et al. -1998-. New insights on the dynamics of dentin formation. Proceedings of the 6th International Conference on Chemistry and Biology of Mineralized Tissues. Vittel, France.
Heywood BR, Sparks NH, Shellis RP, Weiner S, Mann S -1990-. Ultrastructure, morphology and crystal growth of biogenic and synthetic apatites. Connect Tissue Res25:103–119.
13.
Katchburian E -1973-. Membrane-bound bodies as initiators of mineralization of dentine. J Anat116:285–302.
14.
Kinney JH, Balooch M, Marshall SJ, Marshall GW Jr, Weihs TP -1996-. Hardness and Young’s modulus of human peritubular and intertubular dentine. Arch Oral Biol41:9–13.
15.
Kinney JH, Pople JA, Marshall GW, Marshall SJ -2001-. Collagen orientation and crystallite size in human dentin: a small angle x-ray scattering study. Calcif Tissue Int69:31–37.
16.
Lee S, Kossiva D, Glimcher MJ -1983-. Phosphoproteins of bovine dentin: evidence for polydispersity during tooth maturation. Biochemistry22:2596–2601.
17.
Linde A -1984-. Non-collagenous proteins and proteoglycans in dentinogenesis. In: Dentin and dentinogenesis. Vol. II. Linde A, editor. Boca Raton, FL: CRC Press.
18.
Marcott C, Reeder RC, Paschalis EP, Boskey AL, Mendelsohn R -1999-. Infrared microspectroscopic imaging of biomineralized tissues using a mercury-cadmium-telluride focal plane array detector. Phosphorus, Sulfur, Silicon & Related Elements146:417–420.
19.
Marshall GW Jr, Balooch M, Gallagher R, Gansky SA, Marshall SJ -2001-. Mechanical properties of the dentinoenamel junction: AFM studies of nanohardness, elastic modulus and fracture. J Biomed Mater Res54:87–95.
20.
McKee MD, Zalzal S, Nanci A -1996-. Extracellular matrix in tooth cementum and mantle dentin: localization of osteopontin and other noncollagenous proteins, plasma proteins and glycoconjugates by electron microscopy. Anat Rec245:293–312.
21.
Mendelsohn R, Paschalis EP, Boskey AL -1999-. Infrared spectroscopy, microscopy and microscopic imaging of biomineralizing tissues. Spectra-structure correlations from human iliac crest biopsies. J Biomed Optics4:14–21.
22.
Moss ML -1974-. Studies on dentin. I. Mantle dentin. Acta Anat87:481–507.
23.
Pashley DH -1989-. Dentin: a dynamic substrate—a review. Scanning Microsc3:161–176.
24.
Pienkowski D, Doers TM, Monier-Faugere MC, Geng Z, Camacho NP, Boskey AL, et al. -1997-. Calcitonin alters bone quality in beagle dogs. J Bone Miner Res12:1936–1943.
25.
Rahima M, Tsay TG, Andujar M, Veis A -1988-. Localization of phosphophoryn in rat incisor dentin using immunocytochemical techniques. J Histochem Cytochem36:153–157.
26.
Ten Cate AR -1994-. Oral histology. St. Louis, MO: Mosby Year Book.
27.
Tesch W, Eidelman N, Roschger P, Goldenberg F, Klaushofer K, Fratzl P -2001-. Graded microstructure and mechanical properties of human crown dentin. Calcif Tissue Int69:147–157.
28.
Veis A -1993-. Mineral-matrix interactions in bone and dentin. J Bone Miner Res8-Suppl 2-:S493–S497.
29.
Weiner S, Veis A, Beniash E, Arad T, Dillon JW, Sabsay B, et al. -1999-. Peritubular dentin formation: crystal organization and the macromolecular constituents in human teeth. J Struct Biol126:27–41.
30.
Wentrup-Byrne E, Armstrong CA, Armstrong RS, Collins BM -1997-. Fourier transform Raman microscopic mapping of the molecular components in a human tooth. J Raman Spectrosc28:151–158.
31.
White SN, Paine ML, Luo W, Sarikaya M, Fong H, Yu A, et al. -2000-. The dentino-enamel junction is a broad transitional zone uniting dissimilar bioceramic composites. J Am Ceram Soc83:238–240.
