Sage Journals: Discover world-class research

Abstract

Surface properties may affect the clinical outcome of titanium dental implants. The aim of the present study was to investigate the effects of 3 different titanium surfaces—smooth (S), sandblasted (SB), and titanium plasma-sprayed (TPS)—on proliferation, differentiation, and apoptosis of human osteoblast-like cells, SaOS-2. Cell proliferation was significantly (p < 0.05) higher on the S surface, and synthesis of extracellular matrix proteins was more abundant on TPS and SB than on S surfaces. Analysis of integrin receptors showed a higher expression of α2, α5, αVβ3, and ß1 on TPS as compared with SB and S surfaces. An increase in alkaline phosphatase activity was detected only on SB and TPS surfaces. Analysis of cell apoptosis did not demonstrate any significant difference among the 3 different surfaces. The results indicate that titanium surface topography affects proliferation and differentiation of osteoblast-like SaOS-2 cells, suggesting that surface properties might be important for bone response around dental implants in vivo.

Get full access to this article

View all access options for this article.

References

Brunette DM -1988-. The effects of implant surface topography on the behaviour of cells. Int J Oral Maxillofac Implants 3:231–246.

Cochran DL -1999-. A comparison of endosseous dental implant surfaces. J Periodontol 70:1523–1539.

Cohen JJ -1993-. Apoptosis. Immunol Today 14:126–130.

Cooper LF -2000-. A role for surface topography in creating and maintaining bone at titanium endosseous implants. J Prosthet Dent 84:522–534.

Davies JE -1998-. Mechanisms of endosseous integration. Int J Prosthodont 11:391–401.

Degasne I, Basle MF, Demais V, Hure G, Lesourd M, Grolleau B, et al. -1999-. Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells -SaOS-2- on titanium surfaces. Calcif Tissue Int 64:499–507.

Farley JR, Hall SL, Herring S, Tarbaux NM, Matsuyama T, Wergedal JE -1991-. Skeletal alkaline phosphatase activity is an index of the osteoblastic phenotype in subpopulations of the human osteosarcoma cell line SaOS-2. Metabolism 40:664–671.

Lian JB, Stein GS -1993-. The developmental stages of osteoblast growth and differentiation exhibit selective responses of genes to growth factors -TGFbeta1- and hormones -vitamin D and glucocorticoids-. J Oral Implantol 19:95–105.

Martin JY, Schwartz Z, Hummert TW, Schraub DM, Simpson J, Lankford J Jr, et al. -1995-. Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells -MG63-. J Biomed Mater Res 29:389–401.

10.

Matsunaga T, Kojo T, Tsujisawa T, Fukuizumi T, Wada S, Uchida Y, et al. -2001-. Preferential degradation of osteoclasts by titanium tetrachloride. J Biomed Mater Res 55:313–319.

11.

Mosmann T -1983-. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63.

12.

Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, et al. -1990-. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143:420–430.

13.

Postiglione L, Domenico GD, Montagnani S, Spigna GD, Salzano S, Castaldo C, et al. -2003-. Granulocyte-macrophage colony-stimulating factor -GM-CSF- induces the osteoblastic differentiation of the human osteosarcoma cell line SaOS-2. Calcif Tiss Int 72:85–97.

14.

Rodan SB, Imai Y, Thiede MA, Wesolowski G, Thompson D, Bar-Shavit Z, et al. -1987-. Characterization of a human osteosarcoma cell line -SaOS-2- with osteoblastic properties. Cancer Res 47:4961–4966.

15.

Schwartz Z, Lohmann CH, Oefinger J, Bonewald LF, Dean DD, Boyan BD -1999-. Implant surface characteristics modulate differentiation behavior of cells in the osteoblastic lineage. Adv Dent Res 13:38–48.

16.

Springer TA -1990-. Adhesion receptors of the immune system. Nature 346:425–434.

17.

Stanford CM, Keller JC -1991-. The concept of osseointegration and bone matrix expression. Crit Rev Oral Biol Med 2:83–101.

18.

Swart KM, Keller JC, Wightman JP, Draughan RA, Stanford CM, Michaels CM -1992-. Short-term plasma-cleaning treatments enhance in vitro osteoblast attachment to titanium. J Oral Implant 18:130–137.

Behavior of SaOS-2 Cells Cultured on Different Titanium Surfaces

Abstract

Get full access to this article

References