Sage Journals: Discover world-class research

Abstract

Here we report that successful bone formation with a vascular flap inside a cylindrical mold was induced from fat tissue with the use of recombinant human bone morphogenetic protein-2 in rats. Fat tissue connected to blood vessels was prepared to fit into the mold and implanted intramuscularly into the hind leg in Wistar rats. RhBMP-2 (20 μg) was applied in a collagen sheet previously placed on the inside surface of the mold. Bone formation was confirmed radiologically and morphologically at 2, 4, and 8 weeks after the surgery. In the control group without rhBMP-2 or the group with ligation of the blood vessels before the implantation, bone formation was not observed. Our success in bone formation having a definite size, shape, and blood supply may lead to a therapeutic approach to effective bone reconstitution. The present study is the first report on bone induction from fat tissue by rhBMP-2 in vivo.

Get full access to this article

View all access options for this article.

References

Alam MI, Asahina I, Seto I, Oda M, Enomoto S -2001-. Prefabricated vascularized bone flap: a tissue transformation technique for bone reconstruction. Plast Reconstr Surg 108:952–958.

DeLuca L, Raszewski R, Tresser N, Guyuron B -1997-. The fate of preserved autogenous bone graft. Plast Reconstr Surg 99:1324–1328.

Halvorsen YD, Franklin D, Bond AL, Hitt DC, Auchter C, Boskey AL, et al. -2001-. Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells. Tissue Eng 7:729–741.

Huang JI, Beanes SR, Zhu M, Lorenz HP, Hedrick MH, Benhaim P -2002-. Rat extramedullary adipose tissue as a source of osteochondrogenic progenitor cells. Plast Reconstr Surg 109:1033–1041.

Kusumoto K, Bessho K, Fujimura K, Akioka J, Ogawa Y, Iizuka T -1998-. Prefabricated muscle flap including bone induced by recombinant human bone morphogenetic protein-2: an experimental study of ectopic osteoinduction in a rat latissimus dorsi muscle flap. Br J Plast Surg 51:275–280.

Lecoeur L, Ouhayoun JP -1997-. In vitro induction of osteogenic differentiation from non-osteogenic mesenchymal cells. Biomaterials 18:989–993.

Mankani MH, Krebsbach PH, Satomura K, Kuznetsov SA, Hoyt R, Robey PG -2001-. Pedicled bone flap formation using transplanted bone marrow stromal cells. Arch Surg 136:263–270.

Taylor GI -1983-. The current status of free vascularized bone grafts. Clin Plast Surg 10:185–209.

Terheyden H, Jepsen S, Rueger DR -1999-. Mandibular reconstruction in miniature pigs with prefabricated vascularized bone grafts using recombinant human osteogenic protein-1: a preliminary study. Int J Oral Maxillofac Surg 28:461–463.

10.

Terheyden H, Warnke P, Dunsche A, Jepsen S, Brenner W, Palmie S, et al. -2001-. Mandibular reconstruction with prefabricated vascularized bone grafts using recombinant human osteogenic protein-1: an experimental study in miniature pigs. Part II: transplantation. Int J Oral Maxillofac Surg 30:469–478.

11.

Vögelin E, Jones NF, Lieberman JR, Baker JM, Tsingotjidou AS, Brekke JH -2002-. Prefabrication of bone by use of a vascularized periosteal flap and bone morphogenetic protein. Plast Reconstr Surg 109:190–198.

12.

Weiland AJ, Phillips TW, Randolph MA -1984-. Bone grafts: a radiologic, histologic, and biomechanical model comparing autografts, allografts, and free vascularized bone grafts. Plast Reconstr Surg 74:368–379.

13.

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al. -2001-. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7:211–228.

Bone with a Vascular Flap Induced from Fat Tissue with the Use of rhBMP-2 in Rats

Abstract

Get full access to this article

References