The influence of properties of calcium phosphate (CaP) coatings on bone cell activity and bone-implant osseointegration is not well-established. This study investigated the effects of characterized CaP coatings of various heat treatments on osteoblast response. It was hypothesized that heat treatments of CaP coatings alter the initial osteoblast attachment. The 400°C heat-treated coatings were observed to exhibit poor crystallinity and significantly greater phosphate or apatite species compared with as-sputtered and 600°C heat-treated coatings. Similarly, human embryonic palatal mesenchyme (HEPM) cells, an osteoblast precursor cell line, seeded on 400°C heat-treated coatings, exhibited significantly greater cell attachment compared with Ti surfaces, as-sputtered coatings, and 600°C heat-treated coatings. The HEPM cells on Ti surfaces and heat-treated coatings were observed to attach through filopodia, and underwent cell division, whereas the cells on as-sputtered coatings displayed fewer filopodia extensions and cell damage. Analysis of the data suggested that heat treatment of CaP coatings affects cell attachment.
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References
1.
Adams CS, Mansfield K, Perlot RL, Shapiro IM -2001-. Matrix regulation of skeletal cell apoptosis. Role of calcium and phosphate ions. J Biol Chem276:20316–20322.
2.
Chang YL, Stanford CM, Wefel JS, Keller JC -1999-. Osteoblastic cell attachment to hydroxyapatite-coated implant surfaces in vitro. Int J Oral Maxillofac Implants14:239–247.
3.
Christoffersen J -1980-. Kinetics of dissolution of calcium hydroxyapatite. III: Nucleation controlled dissolution of a polydisperse sample of crystals. J Cryst Growth49:29–44.
4.
Cooley DR, Van Dellen AF, Burgess JO, Windeler AS -1992-. The advantages of coated titanium implants prepared by radiofrequency sputtering from hydroxyapatite. J Prosthet Dent67:93–100.
5.
Dasarathy H, Riley C, Coble HD -1993-. Analysis of apatite deposits on substrates. J Biomed Mater Res27:477–482.
6.
de Groot K, Wolke JGC, Jansen JA -1994-. State of the art: hydroxylapatite coatings for dental implants. J Oral ImplantolXX:232&-234.
7.
Ferraz MP, Knowles JC, Olsen I, Monteiro FJ, Santos JD -1999-. Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P2O5 coatings. J Biomed Mater Res47:603–611.
8.
Filiaggi MJ, Coombs NA, Pilliar RM -1991-. Characterization of the interface in the plasma-sprayed HA coating/Ti-6Al-4V implant system. J Biomed Mater Res25:1211–1229.
9.
Gross KA, Berndt CC, Goldschlag DD, Iacono VJ -1997-. In vitro changes of hydroxyapatite coatings. Int J Oral Maxillofac Implants12:589–597.
10.
Haman JD, Lucas LC, Crawmer D -1995-. Characterization of high velocity oxy-fuel combustion sprayed hydroxyapatite. Biomaterials16:229–237.
11.
Herman H -1988-. Plasma spray deposition processes. MRS Bull12:60–67.
12.
Lacefield WR, Lucas LC, Rigney ED, Ong JL, Chen TS, Gantenberg J -1993-. Sputter deposition of calcium phosphate coatings for biomedical applications. In: Surface modification technologies. Vol. VI. Sudarshan TS, Braza JF, editors. Warrendale, Pennsylvania: Minerals, Metals and Materials Society, pp. 591-604.
13.
Lucas LC, Lacefield WR, Ong JL, Whitehead RY -1993-. Calcium-phosphate coatings for dental and medical implants. Coll Surf77:141–147.
14.
Nancollas GH, Tucker BE -1994-. Dissolution kinetics characterization of hydroxyapatite coatings on dental implants. J Oral Implantol20:221–226.
15.
Nimb L, Gotfredsen K, Steen Jensen J -1993-. Mechanical failure of hydroxyapatite-coated titanium and cobalt-chromium-molybdenum alloy implants. An animal study. Acta Orthop Belg59:333–338.
16.
Okamoto K, Matsuura T, Hosokawa R, Akagawa Y -1998-. RGD peptides regulate the specific adhesion scheme of osteoblasts to hydroxyapatite but not to titanium. J Dent Res77:481–487.
17.
Ong JL, Lucas LC -1994-. Post-deposition heat treatments for ion beam sputter deposited calcium phosphate coatings. Biomaterials15:337–341.
18.
Ong JL, Harris LA, Lucas LC, Lacefield WR, Rigney D -1991-. X-ray photoelectron spectroscopy characterization of ion beam sputter deposited calcium phosphate coatings. J Am Ceram Soc74:2301–2304.
19.
Ong JL, Lucas LC, Lacefield WR, Rigney ED -1992-. Structure, solubility and bond strength of thin calcium phosphate coatings produced by ion beam sputter deposition. Biomaterials13:249–254.
20.
Ong JL, Raikar GN, Smoot TM -1997-. Properties of calcium phosphate coatings before and after exposure to simulated biological fluid. Biomaterials18:1271–1275.
21.
Ong JL, Hoppe CA, Cardenas HL, Cavin R, Carnes DL, Sogal A, et al. -1998-. Osteoblast precursor cell activity on HA surfaces of different treatments. J Biomed Mater Res39:176–183.
22.
Ong JL, Bessho K, Cavin R, Carnes DL -2002-. Bone response to radio frequency sputtered calcium phosphate implants and titanium implant in vivo. J Biomed Mater Res59:184–190.
23.
Palka V, Postrkova E, Koerten HK -1998-. Some characteristics of hydroxylapatite powder after plasma spraying. Biomaterials19:1763–1772.
24.
Steflik DE -1994-. Hydroxylapatite coatings on dental implants: benefits and risks. J Oral ImplantolXX:198-200.
25.
Takebe J, Itoh S, Okada J, Ishibashi K -2000-. Anodic oxidation and hydrothermal treatment of titanium results in a surface that causes increased attachment and altered cytoskeletal morphology of rat bone marrow stromal cells in vitro. J Biomed Mater Res51:398–407.
26.
ter Brugge PJ, Jansen JA -2002-. Initial interaction of rat bone marrow cells with non-coated and calcium phosphate coated titanium substrates. Biomaterials23:3269–3277.
27.
ter Brugge PJ, Wolke JG, Jansen JA -2002-. Effect of calcium phosphate coating crystallinity and implant surface roughness on differentiation of rat bone marrow cells. J Biomed Mater Res60:70–78.
28.
Thomas KA, Cook SD -1985-. An evaluation of variables influencing implant fixation by direct bone apposition. J Biomed Mater Res19:875–901.
29.
Wolke JG, van Dijk K, Schaeken HG, de Groot K, Jansen JA -1994-. Study of the surface characteristics of magnetron-sputter calcium phosphate coatings. J Biomed Mater Res28:1477–1484.
30.
Yang Y, Ong JL -2003-. Bond strength, compositional and structural properties of HA coating on Ti substrate, ZrO2-coated Ti substrate, and TPS-coated Ti substrate. J Biomed Mater Res64:509–516.
