Microcalorimetric Measurements of Platelet Metabolism After Synthetic Arterial Bypass Procedures in Sheep and the Effect of Low Molecular Weight Dextran
Restricted accessResearch articleFirst published online July, 1988
Microcalorimetric Measurements of Platelet Metabolism After Synthetic Arterial Bypass Procedures in Sheep and the Effect of Low Molecular Weight Dextran
The overall metabolic activity in platelets after vascular grafting procedure with polytetrafluoroethylene arterial grafts was studied in 18 sheep by use of microcalorimetric measurements. It was found that the heat production (P) from platelets was abnormally low in grafted animals, P=51±8 fW/cell com pared with nongrafted controls, P=60±11 fW/cell, p < 0.001 (fW = 10-15 W). When low molecular weight dextran (LMWD) was given, a significantly higher heat production was observed, P =132 ± 6 fW/cell, thus suggesting a direct platelet effect by the dextran. Platelet aggregation response was decreased after grafting without statistically significant differences between grafted control ani mals and LMWD-treated animals. After the grafting procedure, a drop in platelet count was noted, but it was less pronounced in the LMWD-treated group.
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References
1.
Levin K.: Heat production by leucocytes and thrombocytes measured with a flow microcalorimetric method in normal man and during thyroid dysfunction. Clin Chim Acta32:87-94, 1971.
2.
Monti M., Wadso I.: Microcalorimetric studies of human platelet metabolism at rest. Influence of pH, temperature, cell concentrations, preparation methods and storage. Scand J Hematol19:111-115, 1977.
3.
Spink C., Wadso I.: Calorimetry as an analytical tool in biochemistry and biology. In: Methods of Biochemical Analysis, ed. by Glick G.New York, Wiley-Interscience , 1976, 23:1-59.
4.
Bandman U., Monti M., Wadso I.: Microcalorimetric measurements of heat production in whole blood and blood cells and normal persons. Scand J Clin Lab Invest35:121-127, 1975.
5.
Boyo HE, Ikomi-Kumn JA: Increased metabolic heat production by erythrocytes in sickle-cell disease . LancetI:1215, 1972.
6.
Harker LA, Hondin RI: The role of platelets in acute vascular events. Circulation62 (Suppl): V13-V18, 1980.
7.
Harker LA, Slichter SI, Sauvage LR: Platelet consumption by arterial prostheses. The effect of endothelialization and pharmacologic inhibitors of platelet function. Ann Surg186:594-601, 1977.
8.
Monti M., Fagher B.: A microcalorimetric measurement of heat production in platelets of patients with prosthetic heart valves. Clin Chim Acta102:53-89, 1980.
9.
Murer EH, Hellem AJ, Rozenberg ML: Energy metabolism and platelet function. Scand J Clin Lab Invest19:280-282, 1967.
10.
Ross PD, Fletcher AP, Jamieson GA: Microcalorimetric study of isolated blood platelets in the presence of thrombin and other aggregating agents. Biochem Biophys Acta313:160-118, 1973.
11.
Karpatkin S. : Heterogeneity of human platelets. I. Metabolic and kinetic evidence suggestive of young and old platelets. J Clin Invest48:1073-1082, 1969.
12.
Mason RG, Read MJ, Sheriner RW: Comparison of certain functions of human platelets separated from blood by various means. Am J Pathol76:323-332, 1974.
13.
Bennet PN, Dhall DP, McKenzie FN, et al: Effects of dextran infusion on the adhesiveness of human blood platelets. Lancet2:1001-1003, 1966 .
14.
Christenson JT, Al-Huneidi W., Owunwanne A.: Early platelet distribution in various graft materials. Surg ResComm1:151-158, 1987.
15.
Al-Huneidi W., Owunwanne A., Christenson JT: The effect of low molecular weight dextran on early platelet deposition onto vascular grafts. Intemat Angiol (submitted).
