Rapid and Biologically Safe Procedures for the Evaluation of Antigen-Specific T Cell Response to Microbial Pathogens that May be Used in the BSL-3 and BSL-4 Environment
Free accessResearch articleFirst published online March, 2008
Rapid and Biologically Safe Procedures for the Evaluation of Antigen-Specific T Cell Response to Microbial Pathogens that May be Used in the BSL-3 and BSL-4 Environment
T cell response precedes serological response, and is more feasible than pathogen
detection by molecular tools. Thus, monitoring T cell response to class-A pathogens may
allow for a timely treatment and a correct handling of patients containment issues, after
exposure to potentially lethal agents, such as biological threat, or emerging pathogens.
In this paper, we describe a procedure for the evaluation of T cell response to microbial
pathogens, including class-A pathogens in BSL-3 and BSL-4 equipped laboratories.
FasanellaA., LositoS., AdoneR., CiuchiniF., TrottaT. & AltamuraS.
A. (2003). PCR
assay to detect Bacillus anthracis spores in heat-treated specimens. J. Clin. Microbiol., 41, 896–899.
6.
FlickR., FlickK., FeldmannH., & ElghF. (2003). Reverse genetics for crimean-congo hemorrhagic fever
virus. J. Virol., 77, 5997–6006.
7.
GioiaC., HorejshD., AgratiC., MartiniF., CapobianchiM. R., IppolitoG., & PocciaF. (2005). T cell response profiling to biological threat
agents including the SARS coronavirus. Int. J. Immunopathol.
Pharmacol., 18, 525–530.
LalR. B., EdisonL. J., & ChusedT.
M. (1988). Fixation and
long-term storage of human lymphocytes for surface marker analysis by flow
cytometry. Cytometry, 9, 213–219.
10.
LoY. M., & ChanK. C. (2006). Setting up a polymerase chain reaction laboratory.
Methods Mol. Biol., 336, 11–18.
11.
MahantyS., KalwarR., & RollinP.
E. (1999). Cytokine
measurement in biological samples after physicochemical treatment for inactivation of
biosafety level 4 viral agents. J. Med. Virol., 59, 341–345.
12.
PocciaF., GioiaC., MontesanoC., MartiniF., HorejshD., CastillettiC., PucilloL., CapobianchiM.
R., & IppolitoG. (2003). Flow cytometry and T cell response monitoring after
smallpox vaccination. Emerg. Infect. Dis., 9, 1468–1470.
13.
StroherU., WestE., BuganyH., KlenkH. D., SchnittlerH.
J., & FeldmannH. (2001). Infection and activation of monocytes by Marburg and
Ebola viruses. J. Virol., 75, 11025–11033.
