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Abstract

Ultraviolet germicidal irradiation (UVGI) lamps are used in biological safety cabinets and laboratory containment rooms as methods of surface decontamination. Although some organizations have discouraged the use of UVGI for disinfection, many scientists continue to request this method. The objective of this study was to assess the reliability of using UVGI produced from UV ceiling lamps as an effective method to decrease microbial agent contamination from floor surfaces in a biosafety level 3 laboratory. Actively growing Bacillus cereus and B. anthracis vegetative cells or spores (Sterne strain-Veterinary Vaccine Formula) were used as biological indicators to assess UVGI effects within the containment laboratory. Studies were conducted using UVGI exposure times ranging from 15 minutes to 2 hours with varying inoculums ranging from 10³ to 10⁹ colony-forming unit per sample. Later in the study an UVGI radiometer was used to determine the UVGI intensity and to measure the correlation between the biological indicator results and mechanical instrument data. Study results showed that ceiling-mounted UVGI lamps were effective in reducing the viability of both B. cereus and B. anthracis vegetative cells and spores after a minimum UVGI exposure time of 1 hour at an intensity as low as 8 uW/cm². Additionally, an UVGI radiometer could be used to determine UVGI effectiveness and a UVGI intensity of 8 uW/cm² to 42 uW/cm² corresponded with the increased disinfection observed.

References

American Biological Safety Association. (2000). Position paper on the use of ultraviolet lights in biological safety cabinets. Unpublished draft, ABSA Technical Research Committee.

Banrud

, Moan

(2000). Use of short wave ultraviolet radiation for disinfection in operating rooms. (Comment). Tidsskr Nor Lægeforen, 120, 953.

Blatchley

, Peel

(2001). Disinfection by ultraviolet irradiation. In Block

S. S.

(Ed.), Disinfection, sterilization, and preservation (pp. 823–848) (5th ed.). Philadelphia: Lippincott, Williams & Wilkins.

Botzenhart

, Ruden

, Tolon

, von Scharfenberg

(1976). Clinical uses of ultraviolet light radiation. Praktische Anastesie, 11, 320–327.

Centers for Disease Control and Prevention and Centers for Medicare and Medicaid Services, Health and Human Services. (2003). Clinical Laboratory Improvement Act of 1988. 42 CFR Part 493, Final Rule.

Dietz

, Bohm

, Strauch

(1980). Investigations on disinfection and sterilization of surfaces by ultraviolet radiation. Zentralbl Bakteriol Mikrobiol Hyg [B], 171, 158–167.

Donald

, Lauer

J. L.

(1995). Decontamination, sterilization, disinfection, and antisepsis. In Fleming

, Richardson

, Tulis

, Vesley

(Eds.), Laboratory safety: Principles and practices (pp. 219–238). Washington, DC: ASM Press.

Dulbecco

(1980). Organization, alteration, and expression of the genetic information. In Davis

, Dulbecco

, Eisen

, Ginsberg

(Eds.), Microbiology (3rd ed.). (pp. 183–221). Hagerstown, MD: Harper & Row.

First

K. M.

, Weker

R. A.

, Yasui

, Nardell

E. A.

(2005). Monitoring human exposures to upper-room germicidal ultraviolet irradiation. Journal Occupational Environmental Hygiene, 2, 285–292.

10.

Green

C. F.

, Scarpino

P. V.

, Jensen

N. J.

, Gibb

S. G.

(2004). Disinfection of selected Aspergillus spp. using ultraviolet germicidal irradiation. Canadian Journal of Microbiology, 50, 221–224.

11.

, First

, Burge

(2002). The characterization of upper-room ultraviolet germicidal irradiation in inactivating airborne microorganisms. Environmental Health Perspective, 110, 95–101.

12.

Lai

K. M.

, Burge

H. A.

, First

M. W.

(2004). Size and UV germicidal irradiation susceptibility of Serratia marcescens when aerosolized from different suspending media. Applied Environmental Microbiology, 70, 2021–2027.

13.

Menzies

, Popa

, Hanley

J. A.

, Rand

, Milton

D. K.

(2003). Effect of ultraviolet germicidal lights installed in office ventilation systems on workers' health and well-being: A double-blind multiple crossover trial. Lancet, 362, 1785–1791.

14.

Myatt

, Johnston

, Rudnick

, Milton

(2003). Airborne rhinovirus detection and effect of ultraviolet irradiation on detection by a semi-nested RT-PCR assay. BioMed Central Public Health, 3, 5.

15.

Nicas

, Miller

(1999). A multi-zone model evaluation of the efficacy of upper-room air ultraviolet germicidal irradiation. Applied Occupational Environmental Hygiene, 14, 317–328.

16.

Riley

, Knight

, Middlebrook

(1976). Ultraviolet susceptibility of BCG and virulent tubercle bacilli. American Review of Respiratory Disease, 113, 413–418.

17.

Rudnick

S. N.

(2001). Predicting the ultraviolet radiation distribution in a room with multi-louvered germicidal fixtures. American Industrial Hygiene Association Journal, 62, 434–445.

18.

Talbot

, Jensen

, Moffat

, Wells

(2002). Occupational risk from ultraviolet germicidal irradiation (UVGI) lamps. International Journal Tuberculosis and Lung Disease, 6, 647–648.

19.

U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, & National Institutes of Health. (2000). Primary containment for biohazards: Selection, installation and use of biological safety cabinets (2nd ed.). Richmond

J. Y.

, McKinney

R. W.

(Eds.). Washington, DC: U.S. Government Printing Office. Available at www.cdc.gov/od/ohs/biosfty/bsc/bsc.htm

20.

U.S. Department of Health, Education and Welfare. (1972). Criteria for a recommended standard-occupational exposure to ultra-violet radiation. Public Health Service, Document HSM-73-11009.

21.

Varnam

, Evans

(1991). Foodborne pathogens: An illustrated text. Aylesbury, England: Wolfe Publishing Ltd.

Biological Monitoring of Ultraviolet Germicidal Irradiation in a Biosafety Level 3 Laboratory

Abstract

References