Abstract
The Nanyang Technological University School of Biological Sciences Biosafety Level 3 (BSL-3) laboratory is set up as a core facility for research work involving biological materials of potential biohazard. Many human pathogens—viruses, fungi, parasites, rickettsia, and bacteria are classified as Risk Group 3 microorganisms in the Singapore national guidelines that require a BSL-3 facility for their storage and handling. A comprehensive approach to biosafety is based on a combination of administrative controls, safety practices and procedures, engineering controls, and personal protective equipment. For the benefit of other research institutes and future users in Singapore, here we report on facility requirements and implementation, management of biosafety and biosecurity measures, application of processes and procedures, and documentation specifically adopted in our facility.