The origin of life is yet a compelling scientific mystery that has sometimes been attributed to high-pressure impacts by small solar system bodies such as comets, meteoroids, asteroids, and transitional objects. High-pressure torsion (HPT) is an innovative method with which to simulate the extreme conditions of astronomical impacts and offers insights relevant to prebiotic chemistry. In the present study, we investigated the polymerization and stability of adenosine monophosphate (AMP), a key precursor to ribonucleic acid (RNA), in dry and hydrated conditions (10 wt% water) under 6 GPa at ambient and boiling water temperatures. Comprehensive analyses with the use of X-ray diffraction, Raman spectroscopy, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, scanning electron microscopy, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry revealed no evidence of polymerization, while AMP partly transformed to other organic compounds such as nucleobase-derived fragments of adenine, phosphoribose fragments, dehydrated adenosine, protonated adenosine, and oxidized adenosine. The torque measurements during HPT further highlighted the mechanical behavior of AMP under extreme conditions. These findings suggest that, while HPT under the conditions tested does not facilitate polymerization, the formation of various compounds from AMP confirms the significance of astronomical impacts on the prebiotic chemistry of RNA on early Earth. Key Words: Ribonucleic acid (RNA)—Origin of life—Phase transformations—Chemical reactions—Small solar system bodies. Astrobiology 26, 1–9.
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References
1.
Arranz-MascarosP, BazzicalupiC, BianchiA, et al.Binding and recognition of AMP, ADP, ATP and related inorganic phosphate anions by a tren-based ligand containing a pyrimidine functionality. New J Chem, 2011; 35(9):1883–1891.
2.
BennerS, KimH, CarriganM. Asphalt, water, and the prebiotic synthesis of ribose, ribonucleosides, and RNA. Acc Chem Res, 2012; 45(12):2025–2034.
3.
BlankJG, MillerGH, AhrensMJ, et al.Experimental shock chemistry of aqueous amino acid solutions and the cometary delivery of prebiotic compounds. Orig Life Evol Biosph, 2001; 31(1–2):15–51.
4.
BridgmanPW. Effects of high shearing stress combined with high hydrostatic pressure. Phys Rev, 1935; 48(10):825–847.
5.
Cade-MenunBJ. Improved peak identification in 31P-NMR spectra of environmental samples with a standardized method and peak library. Geoderma, 2015; 257–258:102–114.
6.
ChoJ, KimS. Selective sensing of adenosine monophosphate (AMP) by a calix[6]triazolium-based colorimetric sensing ensemble. RSC Adv, 2022; 12(51):32784–32789.
ČížekJ, JanečekM, VlasákT, et al.The development of vacancies during severe plastic deformation. Mater Trans, 2019; 60(8):1533–1542.
9.
EdalatiK, HoritaZ. A review on high-pressure torsion (HPT) from 1935 to 1988. Mater Sci Eng A, 2016; 652:325–352.
10.
EdalatiK, BachmaierA, BeloshenkoVA, et al.Nanomaterials by severe plastic deformation: Review of historical developments and recent advances. Mater Res Lett, 2022a;10(4):163–256.
11.
EdalatiK, TaniguchiI, FlorianoR, et al.Glycine amino acid transformation under impacts by small solar system bodies, simulated by high-pressure torsion method. Sci Rep, 2022b;12(1):5677.
12.
EdalatiK, AhmedAQ, AkramiS, et al.Severe plastic deformation for producing superfunctional ultrafine-grained and heterostructured materials: An interdisciplinary review. J Alloys Compd, 2024a;1002:174667.
13.
EdalatiK, Hidalgo-JiménezJ, NguyenTT, et al.High-pressure torsion processing of serine and glutamic acid: Understanding mechanochemical behavior of amino acids under astronomical impacts. Adv Eng Mater, 2024b;26(19):2302267.
14.
FerrisJP, HillARJr, LiuR, et al.Synthesis of long prebiotic oligomers on mineral surfaces. Nature, 1996; 381(6577):59–61.
15.
FuH, XuL, LvQ, et al.Electrospray ionization mass spectra of amino acid phosphoramidates of adenosine. Rapid Commun Mass Spectrom, 2000; 14(19):1813–1822.
16.
FurukawaT, SekineY, ObaM, et al.Nucleobase synthesis in interstellar ices. Nat Commun, 2019; 10:4413.
17.
GlavinDP, CallahanMP, DworkinJP, et al.The effects of parent body processes on amino acids in carbonaceous chondrites. Meteorit Planet Sci, 2010; 45(12):1948–1972.
18.
GogichaevaNV, AltermanMA. Amino acid analysis by means of MALDI TOF mass spectrometry or MALDI TOF/TOF tandem mass spectrometry. In: Amino Acid Analysis. Methods in Molecular Biology 2019;Vol. 2030. (AltermanM. eds.) Humana: New York; 2019; pp. 17–32.
19.
HazenRM, SverjenskyDA. Mineral surfaces, geochemical complexities, and the origins of life. Cold Spring Harb Perspect Biol, 2010; 2(5):a002162.
20.
KitadaiN, MaruyamaS. Origins of building blocks of life: A review. Geosci Front, 2018; 9(4):1117–1153.
21.
KitajimaM. Defects in crystals studied by Raman scattering. Crit. Rev. Solid State Mater Sci, 1997; 22(4):275–349.
22.
KroonblawdMP, LindseyRK, GoldmanN. Synthesis of functionalized nitrogen-containing polycyclic aromatic hydrocarbons and other prebiotic compounds in impacting glycine solutions. Chem Sci, 2019; 10(24):6091–6098.
23.
KunduJ, NeumannO, JaneskoBG, et al.Adenine-and adenosine monophosphate (AMP)-gold binding interactions studied by surface-enhanced Raman and infrared spectroscopies. J Phys Chem C, 2009; 113(32):14390–14397.
24.
LiuB, NielsenSB, HvelplundP, et al.Collision-induced dissociation of hydrated adenosine monophosphate nucleotide ions: Protection of the ion in water nanoclusters. Phys Rev Lett, 2006; 97(13):133401–133404.
25.
MartinsZ, BottaO, FogelML, et al.Extraterrestrial nucleobases in the Murchison meteorite. Earth Planet Sci Lett, 2008; 270(1–2):130–136.
26.
MartinsZ, PriceMC, GoldmanN, et al.Shock synthesis of amino acids from impacting cometary and icy planet surface analogues. Nature Geosci, 2013; 6(12):1045–1049.
27.
MastCB, SchinkS, GerlandU, et al.Escalation of polymerization in a thermal gradient. Proc Natl Acad Sci U S A, 2013; 110(20):8030–8035.
28.
Nagana GowdaGA, AbellL, LeeCF, et al.Simultaneous analysis of major coenzymes of cellular redox reactions and energy using ex vivo 1H NMR spectroscopy. Anal Chem, 2016; 88(9):4817–4824.
29.
OberdorferB, LorenzoniB, UngerK, et al.Absolute concentration of free volume-type defects in ultrafine-grained Fe prepared by high-pressure torsion. Scr Mater, 2010; 63(4):452–455.
30.
OrgelLE. Prebiotic chemistry and the origin of the RNA world. Crit Rev Biochem Mol Biol, 2004; 39(2):99–123.
31.
PatelBH, PercivalleC, RitsonDJ, et al.Common origins of RNA, protein, and lipid precursors in a Cyanosulfidic Protometabolism. Nat Chem, 2015; 7(4):301–307.
32.
PippanR, ScheriauS, TaylorA, et al.Saturation of fragmentation during severe plastic deformation. Annu Rev Mater Res, 2010; 40(1):319–343.
33.
PownerMW, GerlandB, SutherlandJD. Synthesis of activated pyrimidine ribonucleotides in prebiotically plausible conditions. Nature, 2009; 459(7244):239–242.
34.
QiJ, PanY, LuoZ, et al.Facile and scalable fabrication of bioderived flame retardant based on adenine for enhancing fire safety of fully biodegradable PLA/PBAT/TPS ternary blends. J Appl Polym Sci, 2021; 138(35):1–17.
35.
RakshitS, RayA, TaheriA, et al.Influence of citrate and phosphate on the adsorption of Adenosine-5′-monophosphate at the hematite water interface. Front Environ Sci, 2022; 10:894581.
36.
RimaiL, ColeT, ParsonsJL, et al.Studies of Raman spectra of water solutions of adenosine tri-, di-, and monophosphate and some related compounds. Biophys J, 1969; 9(3):320–329.
37.
RotelliL, Trigo-RodríguezJM, Moyano-CamberoCE, et al.The key role of meteorites in the formation of relevant prebiotic molecules in a formamide/water environment. Sci Rep, 2016; 6:38888.
38.
SasmalAK, PalT. Chromism of chemical compounds. J Indian Chem Soc, 2021; 98(5):100073.
39.
StrzeleckaD, ChmielinskiS, BednarekS, et al.Analysis of mononucleotides by tandem mass spectrometry: Investigation of fragmentation pathways for phosphate- and ribose-modified nucleotide analogues. Sci Rep, 2017; 7(1):8931.
40.
Trigo-RodriguezJM, RimolaA, TanbakoueiS, et al.Accretion of water in carbonaceous chondrites: Current evidence and implications for the delivery of water to early Earth. Space Sci Rev, 2019; 215(1):18.
41.
WangC, JiaoL, MengH, et al.Bifunctional heterogeneous catalysts derived from the coordination of adenosine monophosphate to Sn (IV) for effective conversion of sucrose to 5-hydroxymethylfurfural. Catal Sci Technol, 2022; 12(2):630–640.
42.
YamagakiT, NobuharaM. Structural categorization of adenine, guanine, and xanthine derivatives using matrix-assisted laser desorption/ionization mass spectrometry with 5-nitrosalicylic acid and 1,5-diaminonaphtalene. J Am Soc Mass Spectrom, 2025; 36(2):329–339.
43.
ZhilyaevAP, LangdonTG. Using high-pressure torsion for metal processing: Fundamentals and applications. Prog Mater Sci, 2008; 53(6):893–979.
