Our investigation in Mars-relevant terrestrial environments where biological material is entombed within rapidly precipitated evaporite crystals has given us the ability to evaluate the preservation potential of a hypersaline brine system in advance of interrogating similar environments on Mars. These evaporite minerals, halite (NaCl) and gypsum (CaSO4), have been found to host authigenic fluid inclusions over geologic time, with cellular life and carotenoid pigments that are understudied in the planetary context. Great Salt Lake provides an excellent site to test the ability to detect organic matter in Mars-relevant evaporite crystals. DNA was extracted to determine which microbial clades were present and assess the attenuation of DNA preservation from the host fluid of the lake to the mineral. Raman spectroscopy was used to investigate the presence of pigments that have longer preservation potential than DNA. Compared with the water column, evaporite minerals preserve higher volumes of DNA and associated biochemistry, whereas entombed fluid inclusions preserve even higher magnitudes of both biomarkers. This indicates organic addition and continued preservation as the crystals precipitate from the fluid, which was later confirmed as micrometer-scale environments continued to maintain the ecology within closed-system fluid inclusions. Raman analyses of halite revealed the presence of β-carotene and bacterioruberin, consistent with the presence of carotenoid-generating bacteria and archaea in this hypersaline environment, which are characterized by pink coloration. The continued preservation of these chemical biomarkers over time has led to the formation of physical biosignatures within the evaporite record. Given that these same minerals are present in ancient fluvial sites across Mars, halite and gypsum are ideal candidates for future in situ observation and should be considered high priority for sample return missions.
Almeida-DalmetS, SikaroodiM, GillevetPM, et al.Temporal study of the microbial diversity of the north arm of great salt lake, Utah, US. Microorganisms, 2015; 3(3):310–326; doi: 10.3390/microorganisms3030310
2.
AllwoodA, GrotzingerJP, KnollA, et al.Controls on development and diversity of early Archean stromatolites. Proc Natl Acad Sci U S A, 2009; 106(24):9548–9555; doi: 10.1073/pnas.0903323106
3.
Andrews-HannaJC, ZuberMT, ArvidsonRE, et al.Early Mars hydrology: Meridiani playa deposits and the sedimentary record of Arabia terra. J Geophys Res, 2010; 115:E06002; doi: 10.1029/2009JE003485
4.
AubreyAD, CleavesHJ, BadaJL. The role of submarine hydrothermal systems in the synthesis of amino acids. Orig Life Evol Biosph, 2009; 39(2):91–108; doi: 10.1007/s11084-008-9153-2
5.
AubreyAD, CleavesHJ, ChalmersJH, et al.Sulfate minerals and organic compounds on Mars. Geol, 2006; 34(5):357–360; doi: 10.1130/G22316.1
6.
BadaJL, EhrenfreundF, GrunthanerF, et al.Urey: Mars organic and oxidant detector. Space Sci Rev, 2008; 135(1–4):269–279; doi: 10.1007/s11214-007-9213-3
BarbieriR, StivalettaN. Continental evaporites and the search for evidence of life on Mars. Geol J, 2011; 46(6):513–524; doi: 10.1002/gj.1326
9.
BaxterBK, EddingtonB, RiddleMR, et al.Great Salt Lake halophilic microorganisms as models for astrobiology: Evidence for desiccation tolerance and ultraviolet irradiation resistance. Proc SPIE, 2007; 6694:669415; doi: 10.1117/12.732621
10.
BaxterBK, LitchfieldCD, SowersK, et al.Microbial diversity of Great Salt Lake. In: Adaptation to Life at High Salt Concentrations in Archaea, Bacteria, and Eukarya. Cellular Origin, Life in Extreme Habitats and Astrobiology. (Gunde-CimermanN, OrenA, PlemenitašA eds.) Springer: Dordrecht, Netherlands, 2005; Vol. 9; pp 9–25.
11.
BaxterBK, LitchfieldCD, SowersK, et al.Adaptation to Life in High Salt Concentrations in Archaea Bacteria, and Eukarya. Springer: Dordrecht, Netherlands; 2005.
12.
BaxterBK. Great Salt Lake microbiology: A historical perspective. Int Microbiol, 2018; 21(3):79–95; doi: 10.1007/s10123-018-0008-z
13.
BeegleLW, WilsonMG, FernandoA, et al.A concept for NASA’s Mars 2016 astrobiology field laboratory. Astrobiology, 2007; 7(4):545–577; doi: 10.1089/ast.2007.0153
14.
BibringJ-P, LangevinY, GendrinAet al.Mars surface diversity as revealed by the OMEGA/Mars Express observations. Science, 2005; 307(5715):1576–1581; doi: 10.1126/science.1108806
15.
BridgesJC, SchwenzerSP, LeveilleR, et al.Diagenesis and clay mineral formation at Gale crater, Mars. J Geophys Res Planets, 2015; 120(1):1–19; doi: 10.1002/2014JE004757
16.
CannonJS, CannonMA. The southern pacific railroad trestle—past and present. In: Great Salt Lake: An Overview of Change. (GwynnJW. ed.) Special Publication of the Utah Department of Natural Resources: Salt Lake City, UT, 2002; pp 283–294.
17.
CarterJ, PouletF, BibringJ-Pet al., “Hydrous minerals on Mars as seen by the CRISM and OMEGA imaging spectrometers: Updated global view, Journal of Geophysical Research (Planets), 2013; 118:831–858; doi: 10.1029/2012JE004145
18.
ChoquettePW, PrayLC. Geologic nomenclature and classification of porosity in sedimentary carbonates. Am Assoc Pet Geol Bull, 1970; 54(2):207–250; doi: 10.1306/5D25C98B-16C1-11D7-8645000102C1865D
19.
ChybaC, PhillipsC. Possible ecosystems and the search for life on Europa. Proc Natl Acad Sci U S A, 2001; 98(3):801–804; doi: 10.1073/pnas.98.3.801
20.
ClarkBC, KolbVM, SteeleA, et al.Origin of life on Mars: Suitability and opportunities. Life (Basel), 2021; 11(6):539; doi: 10.3390/life11060539
21.
CohenourRE, ThompsonKC. Geologic setting of Great Salt Lake. In: The Great Salt Lake. Utah Geological Association: Salt Lake City, UT, 1966; pp 35–56.
22.
CockellCS. Trajectories of Martian habitability. Astrobiology, 2014a;14(2):182–203; doi: 10.1089/ast.2013.1106
23.
CockellCS, AndradyAL. The Martian and extraterrestrial UV radiation environment-1. Biological and closed-loop ecosystem considerations. Acta Astronaut, 1999; 44(1):53–62; doi: 10.1016/s0094-5765(98)00186-6
24.
CockellCS. The subsurface habitability of terrestrial rocky planets: Mars. In: Microbial Life of the Deep Biosphere. (Life in Extreme Environments) (KallmeyerJ, WagnerD. eds.) Walter de Gruyter GmbH: Berlin, 2014b; Vol. 1; pp 225–259.
25.
CockellCS, HoltJ, CampbellJ, et al.Subsurface scientific exploration of extraterrestrial environments (MINAR 5): Analog science, technology, and education in the Boulby Mine, UK. Int J Astrobiol, 2019; 18(2):157–182; doi: 10.1017/S1473550418000186
26.
EardleyAJ, StringhamB. Selenite crystals in the clays of Great Salt Lake. J Sediment Petrol, 1952; 22(4):234–238; doi: 10.1306/D4269517-2B26-11D7-8648000102C1865D
27.
Ehling-SchulzM, BilgerW, SchererS. UV-B-induced synthesis of photoprotective pigments and extracellular polysaccharides in the terrestrial cyanobacterium Nostoc commune. J Bacteriol, 1997; 179(6):1940–1945; doi: 10.1128/jb.179.6.1940-1945.1997
28.
EhlmannBL, EdwardsCS. Mineralogy of the Martian Surface. Annu Rev Earth Planet Sci, 2014; 42(1):291–315; doi: 10.1146/annurev-earth-060313-055024
29.
EhlmannBL, MustardJF, MurchieSL, et al.Subsurface water and clay mineral formation during the early history of Mars. Nature, 2011; 479(7371):53–60; doi: 10.1038/nature10582
30.
EhlmannBL, MustardJF, SwayzeGA, et al.Identification of hydrated silicate minerals on Mars using MRO-CRISM: Geologic context near Nili fossae and implications for aqueous alteration. J Geophys Res, 2009; 114(E2):E00D08; doi: 10.1029/2009JE003339
31.
EhrenfreundP, RölingWFM, ThielCS, et al.Astrobiology and habitability studies in preparation for future Mars missions: Trends from investigating minerals, organics and biota. Int J Astrobiol, 2011; 10(3):239–253; doi: 10.1017/S1473550411000140
32.
FendrihanS, MussoM, Stan-LotterH. Raman spectroscopy as a potential method for the detection of extremely halophilic archaea embedded in halite in terrestrial and possibly extraterrestrial samples. J Raman Spectrosc, 2009; 40(12):1996–2003; doi: 10.1002/jrs.2357
33.
GlennieKW. (ed.) Petroleum Geology of the North Sea: Basic Concepts and Recent Advances. Blackwell: London; 1998; pp. 160–75.
34.
GrotzingerJP, ArvidsonRE, BellJFet al.Stratigraphy and sedimentology of a dry to wet eolian depositional system, Burns formation, Meridiani Planum, Mars. Earth and Planetary Science Letters, 2005; 240(1):11–72; doi: 10.1016/j.epsl.2005.09.039
35.
GrotzingerJP, GuptaS, MalinMCet al.Deposition, exhumation, and paleoclimate of an ancient lake deposit, Gale crater, Mars. Science, 2015; 350(6257):aac7575; doi: 10.1126/science.aac7575
36.
HaysLE, GrahamHV, Des MaraisDJet al.Biosignature Preservation and Detection in Mars Analog Environments. Astrobiology, 2017; 17(4):363–400; doi: 10.1089/ast.2016.1627
37.
HerkenhoffKE, SquyresSW, ArvidsonRet al.Evidence from Opportunity's Microscopic Imager for water on Meridiani Planum. Science, 2004; 306(5702):1727–1730; doi: 10.1126/science.1105286
38.
HirabayashiH, IshiiT, TakaichiS, et al.The role of carotenoids in the Photoadaptation of the brown-colored sulfur bacterium Chlorobium phaeobacteroides. Photochem Photobiol, 2004; 79(3):280–285; doi: 10.1562/wb-03-11.1
39.
JehličkaJ, EdwardsHG, OrenA. Raman spectroscopy of microbial pigments. Appl Environ Microbiol, 2014; 80(11):3286–3295; doi: 10.1128/AEM.00699-14
40.
JonesDL, BaxterBK. DNA repair and photoprotection: Mechanisms of overcoming environmental ultraviolet radiation exposure in halophilic archaea. Front Microbiol, 2017; 8:1882; doi: 10.3389/fmicb.2017.01882
41.
KnollAHet al.Veneers, rinds, and fracture fills: Relatively late alteration of sedimentary rocks at Meridiani Planum, Mars. Journal of Geophysical Research: Planets, 2008; 113(E6):E06S16.
42.
KnollAH, CarrM, ClarkB, et al.An astrobiological perspective on Meridiani Planum. Earth Planet Sci Lett, 2005; 240(1):179–189; doi: 10.1016/j.epsl.2005.09.045
43.
LindsayMR, AndersonC, FoxN, et al.Microbialite response to an anthropogenic salinity gradient in Great Salt Lake, Utah. Geobiology, 2017; 15(1):131–145; doi: 10.1111/gbi.12201
44.
LowensteinTK, LaurenAC, DolginkoJG-V. Influence of magmatic hydrothermal activity on brine evolution in closed basins: Searles Lake, California. GSA Bulletin, 2016; 128(9–10):1555–1568; doi: 10.1130/B31398.1
45.
LynchKL, HorganBH, Munakata-MarrJ, et al.Near-infrared spectroscopy of lacustrine sediments in the Great Salt Lake Desert: An analog study for Martian paleolake basins. JGR Planets, 2015; 120(3):599–623; doi: 10.1002/2014JE004707
46.
MadisonRJ. Effects of a Causeway on the Chemistry of the Brine in Great Salt Lake Utah. Utah Geological and Mineralogical Survey Water-Resources Bulletin 14. USGS: Salt Lake City, UT; 1970.
47.
MancinelliRL, FahlenTF, LandheimR, et al.Brines and Evaporites: Analogs for Martian life. Adv Space Res, 2004; 33(8):1244–1246; doi: 10.1016/j.asr.2003.08.034
48.
Mathews-RothMM, KrinskyNI. Studies on the protective function of the carotenoid pigments of Sarcina lutea. Photochem Photobiol, 1970; 11(6):419–428; doi: 10.1111/j.1751-1097.1970.tb06014.x
49.
McLennanSM, AndersonRB, BellJFet al.Elemental geochemistry of sedimentary rocks at Yellowknife Bay, Gale crater, Mars. Science, 2014; 343(6169):1244734; doi: 10.1126/science.1244734
50.
McLennanSM, BellJFIII, CalvinW, et al.Provenance and diagenesis of the evaporite-bearing burns formation, meridiani planum, Mars. Earth Planet Sci Lett, 2005; 240(1):95–121; doi: 10.1016/j.epsl.2005.09.041
51.
MeunierA, PetitS, EhlmannBL, et al.Magmatic precipitation as a possible origin of Noachian clays on Mars. Nature Geosci, 2012; 5(10):739–743; doi: 10.1038/ngeo1572
52.
MichalskiJR, CuadrosJ, NilesPB, et al.Groundwater activity on Mars and implications for a deep biosphere. Nature Geosci, 2013; 6(2):133–138; doi: 10.1038/ngeo1706
53.
MillikenRE, GrotzingerJP, ThomsonBJ. Paleoclimate of Mars as captured by the stratigraphic record in Gale Crater. Geophysical Research Letters, 2010; 37(4), L04201; doi: 10.1029/2009GL041870
54.
MingDW, ArcherPD, GlavinDPet al.Volatile and organic compositions of sedimentary rocks in Yellowknife Bay, Gale crater, Mars. Science, 2014; 343(6169):1245267; doi: 10.1126/science.1245267
55.
MingD, MittlefehldtD, MorrisRet al.Geochemical and mineralogical indicators for aqueous processes in the Columbia Hills of Gusev crater, Mars. Journal of Geophysical Research-Planets, 2006; 111(E02S12); doi: 10.1029/2005JE002560
56.
MichalskiJR, CuadrosJ, BishopJL, et al.Constraints on the crystal-chemistry of Fe/Mg-rich Smectitic clays on Mars and links to global alteration trends. Earth Planet Sci Lett, 2015; 427:215–225; doi: 10.1016/j.epsl.2015.06.020
57.
MurchieSL, MustardJF, EhlmannBL, et al.A synthesis of Martian aqueous mineralogy after 1 Mars year of observations from the Mars reconnaissance orbiter. J Geophys Res, 2009; 114(E2); doi: 10.1029/2009JE003342
58.
OviattCG, ThompsonRS, KaufmanDS, et al.Reinterpretation of the Burmester core, Bonneville basin, Utah. Quat Res, 1999; 52(2):180–184; doi: 10.1006/qres.1999.2058
59.
PerlSM, McLennanSM, and the Athena Science Team (2008) . Comparison of secondary porosity and permeability from Eagle to Victoria craters, Meridiani Planum, Mars. Lunar and Planetary Science XXXIX, Abstract #2246, Lunar and Planetary Institute, Houston (CD-ROM). http://www.lpi.usra.edu/meetings/lpsc2008/pdf/2246.pdf
60.
PhuaYY, EhlmannB, SiljeströmS, et al.Characterizing hydrated sulfates and altered phases in Jezero crater fan and floor geologic units with SHERLOC on Mars 2020. JGR Planets, 2024; 129(7):e2023JE008251; doi: 10.1029/2023JE008251
61.
PerlSM, BaxterBK. Great Salt Lake as an astrobiology analogue for ancient martian hypersaline aqueous systems. In: Great Salt Lake Biology: A Terminal Lake in a Time of Change. (BaxterBK, ButlerJ., eds.) Springer: Cham, Switzerland; 2020; pp 487–514.
62.
PerlSM, MurphyAE, Govinda RajC, et al.Crystal habits as potential Biosignatures. Astrobiology, 2025; 25(8):525–536; doi: 10.1177/15311074251360767
63.
PerlSM, CelestianAJ, CockellCS, et al.A proposed Geobiology-driven nomenclature for astrobiological in situ observations and sample analyses. Astrobiology, 2021; 21(8):954–967; doi: 10.1089/ast.2020.2318
64.
RamanC, KrishnanK. The negative absorption of radiation. Nature, 1928; 122:12–13; doi: 10.1038/122012b0
65.
SankaranarayananK, LowensteinTK, TimofeeffMN, et al.Characterization of ancient DNA supports long-term survival of Haloarchaea. Astrobiology, 2014; 14(7):553–560; doi: 10.1089/ast.2014.1173
66.
SatterfieldCL, LowensteinTK, VreelandRH, et al.New evidence for 250 Ma age of halotolerant bacterium from a Permian salt crystal. Geol, 2005; 33(4):265–268; doi: 10.1130/G21106.1
67.
SchopfJW, FarmerJD, FosterIS, et al.Gypsum-Permineralized microfossils and their relevance to the search for life on Mars. Astrobiology, 2012; 12(7):619–633; doi: 10.1089/ast.2012.0827
68.
ShroderJF, CornwellK, OviattCG, et al.Landslides, alluvial fans, and dam failure at red rock pass: The outlet of Lake Bonneville. In: Lake Bonneville: A Scientific Update. (OviattCG, ShroderJF eds.) Elsevier: Amsterdam, 2016; pp. 75–85.
69.
SiljeströmS, CzajaAD, CorpolongoA, et al.Evidence of sulfate-rich fluid alteration in Jezero crater floor, Mars. JGR Planets, 2024; 129(1):e2023JE007989; doi: 10.1029/2023JE007989
70.
SmithGI. Subsurface stratigraphy and geochemistry of late quaternary Evaporites, Searles Lake, California, U.S. (Geological Survey Professional Paper 1043). US Geological Survey: Washington, DC; 1979.
71.
SmithGI. Late Cenozoic geology and lacustrine history of Searles valley, Inyo and san Bernardino counties, California: U.S. (Geological Survey Professional Paper 1727). US Geological Survey: Washington, DC; 2009.
72.
SpencerRJ, EugsterHP, JonesBF, et al.Geochemistry of great salt lake, Utah I: Hydrochemistry since 1850. Geochim Cosmochim Acta, 1985; 49(3):727–737; doi: 10.1016/0016-7037(85)90167-X
73.
SquyresSW, KnollAH. Sedimentary rocks at Meridiani Planum: Origin, diagenesis, and implications for life on Mars. Earth Planet Sci Lett, 2005; 240(1):1–10; doi: 10.1016/j.epsl.2005.09.038
74.
StahlW, SiesH. Photoprotection by dietary carotenoids: concept, mechanisms, evidence and future development. Mol Nutr Food Res, 2012; 56(2):287–295; doi: 10.1002/mnfr.201100232
75.
SummonsRE, AmendJP, BishDL, et al.Preservation of Martian organic and environmental records: Final report of the Mars Biosignature working group. Astrobiology, 2011; 11(2):157–181; doi: 10.1089/ast.2010.0506
76.
TehaiM, FranzettiB, MaureletM-C, et al.The search for traces of life: The protective effect of salt on biological macromolecules. Extremophiles, 2002; 6:427–430; doi: 10.1007/s00792-002-0275-6
77.
TruscottGT. New trends in photobiology: The Photophysics and photochemistry of the carotenoids. J Photochem Photobiol B Biology, 1990; 6(4):359–371; doi: 10.1016/1011-1344(90)85110-I
78.
VivianoCE, SeelosFP, MurchieSL, et al.Revised CRISM spectral parameters and summary products based on the currently detected mineral diversity on Mars. JGR Planets, 2014; 119(6):1403–1431; doi: 10.1002/2014JE004627
79.
WadsworthJ, CockellCS. Perchlorates on Mars enhance the bacteriocidal effects of UV light. Sci Rep, 2017; 7:4662; doi: 10.1038/s41598-017-04910-3
80.
WintersYD, LowensteinTK, TimofeeffMN. Identification of carotenoids in ancient salt from Death Valley, Saline Valley, and Searles Lake, California, using laser Raman spectroscopy. Astrobiology, 2013; 13(11):1065–1080; doi: 10.1089/ast.2012.0952
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