Coxiella spp. are widespread in ticks. Coxiella burnetii, the agents of Q fever, is vectored by ticks and is well known for its veterinary and medical importance. However, reports on C. burnetii in ticks from China have been quite few compared with other tick-borne pathogens. In addition to C. burnetii, more and more Coxiella endosymbionts of ticks have been described in China.
Materials, Methods, and Results:
In this study, ticks were collected from domestic animals in two provinces (Chongqing and Xinjiang) in western China, and the Coxiella spp. in them were molecularly studied. In the 168 ticks (all Hyalomma asiaticum) from Xinjiang, C. burnetii was detected with an overall positive rate of 76.19%. In contrast, in 96 ticks (all Rhipicephalus microplus) from Chongqing, only Coxiella endosymbiont was identified with an extremely high prevalence (97.92%).
Conclusions:
We propose that there may have been a tick-animal-tick circulation of C. burnetii in Xinjiang. The high positive rate of C. burnetii suggests a high risk to public health.
AminF, AliS, ImranM, et al.Molecular detection of Coxiella burnetii in ticks infesting wild and domestic animals in the Eastern region of Punjab, Pakistan. Trop Anim Health Prod, 2024; 56(7):252; doi: 10.1007/s11250-024-04087-8
ApanaskevichDA, HorakIG. The genus Hyalomma. XI. Redescription of all parasitic stages of H. (Euhyalomma) asiaticum (Acari: Ixodidae) and notes on its biology. Exp Appl Acarol, 2010; 52(2):207–220; doi: 10.1007/s10493-010-9361-0
4.
BatuN, WangY, LiuZ, et al.Molecular epidemiology of Rickettsia sp. and Coxiella burnetii collected from Hyalomma asiaticum in Bactrian camels (Camelus bactrianus) in inner Mongolia of China. Ticks Tick Borne Dis, 2020; 11(6):101548; doi: 10.1016/j.ttbdis.2020.101548
5.
BrennerAE, Muñoz-LealS, SachanM, et al.Coxiella burnetii and related tick endosymbionts evolved from pathogenic ancestors. Genome Biol Evol, 2021; 13(7):evab108; doi: 10.1093/gbe/evab108
6.
BrindhaS, ShindeSV, BhureM, et al.Occurrence of Coxiellosis in ruminants and its associated risk factors. Acta Trop, 2024; 255:107235; doi: 10.1016/j.actatropica.2024.107235
7.
BuysseM, DuhayonM, CantetF, et al.Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii. PLoS Negl Trop Dis, 2021; 15(1):e0009008; doi: 10.1371/journal.pntd.0009008
8.
CelinaSS, CernýJ. Coxiella burnetii in ticks, livestock, pets and wildlife: A mini-review. Front Vet Sci, 2022; 9:1068129; doi: 10.3389/fvets.2022.1068129
9.
ChisuV, MuraL, FoxiC, MasalaG. Coxiellaceae in ticks from human, domestic and wild hosts from Sardinia, Italy: High diversity of Coxiella-like endosymbionts. Acta Parasitol, 2021; 66(2):654–663; doi: 10.1007/s11686-020-00324-w
10.
Cotes-PerdomoAP, OviedoÁ, CastroLR. Molecular detection of pathogens in ticks associated with domestic animals from the Colombian Caribbean region. Exp Appl Acarol, 2020; 82(1):137–150; doi: 10.1007/s10493-020-00531-0
11.
DraganAL, VothDE. Coxiella burnetii: International pathogen of mystery. Microbes Infect, 2020; 22(3):100–110; doi: 10.1016/j.micinf.2019.09.001
12.
DuanDY, LiuYK, LiuL, et al.Microbiome analysis of the midguts of different developmental stages of Argas persicus in China. Ticks Tick Borne Dis, 2022; 13(1):101868; doi: 10.1016/j.ttbdis.2021.101868
13.
DuanL, ZhangL, HouX, et al.Surveillance of tick-borne bacteria infection in ticks and forestry populations in Inner Mongolia, China. Front Public Health, 2024; 12:1302133; doi: 10.3389/fpubh.2024.1302133
14.
DuronO, JourdainE, McCoyKD. Diversity and global distribution of the Coxiella intracellular bacterium in seabird ticks. Ticks Tick Borne Dis, 2014; 5(5):557–563; doi: 10.1016/j.ttbdis.2014.04.003
15.
DuronO, NoëlV, McCoyKD, et al.The recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the Q fever pathogen, Coxiella burnetii. PLoS Pathog, 2015a;11(5):e1004892; doi: 10.1371/journal.ppat.1004892
16.
DuronO, Sidi-BoumedineK, RoussetE, et al.The importance of ticks in Q fever transmission: What has (and has not) been demonstrated? Trends Parasitol, 2015b;31(11):536–552; doi: 10.1016/j.pt.2015.06.014
17.
GeY, GuoG, GeB, et al.The spleen microbiota of small wild mammals reveals distinct patterns with tick-borne bacteria. PLoS Negl Trop Dis, 2018; 12(7):e0006499; doi: 10.1371/journal.pntd.0006499
18.
GuindonS, DelsucF, DufayardJF, GascuelO. Estimating maximum likelihood phylogenies with PhyML. Methods Mol Biol, 2009; 537:113–137; doi: 10.1007/978-1-59745-251-9_6
19.
JiaoJ, LuZ, YuY, et al.Identification of tick-borne pathogens by metagenomic next-generation sequencing in Dermacentor nuttalli and Ixodes persulcatus in Inner Mongolia, China. Parasit Vectors, 2021; 14(1):287; doi: 10.1186/s13071-021-04740-3
20.
KilicogluY, AkpinarRK, KiziltepeS, et al.Molecular investigation of Coxiella burnetii in the Middle and East Black Sea region in aborted bovine fetuses and investigation of the oxidant/antioxidant system. Comp Immunol Microbiol Infect Dis, 2023; 98:101995; doi: 10.1016/j.cimid.2023.101995
21.
KrzysiakMK, PuchalskaM, OlechW, AnuszK. A Freedom of Coxiella burnetii Infection Survey in European Bison (Bison bonasus) in Poland. Animals (Basel), 2021; 11(3):651; doi: 10.3390/ani11030651
22.
LiJ, LiY, MoumouniPFA, et al.First description of Coxiella burnetii and Rickettsia spp. infection and molecular detection of piroplasma co-infecting horses in Xinjiang Uygur Autonomous Region, China. Parasitol Int, 2020a;76:102028; doi: 10.1016/j.parint.2019.102028
23.
LiY, LiJ, ChahanB, et al.Molecular investigation of tick-borne infections in cattle from Xinjiang Uygur Autonomous Region, China. Parasitol Int, 2020b;74:101925; doi: 10.1016/j.parint.2019.05.003
24.
LuM, TangG, RenZ, et al.Ehrlichia, Coxiella and Bartonella infections in rodents from Guizhou Province, Southwest China. Ticks Tick Borne Dis, 2022b;13(5):101974; doi: 10.1016/j.ttbdis.2022.101974
25.
LuM, TianJ, PanX, et al.Identification of Rickettsia spp., Anaplasma spp., and an Ehrlichia canis-like agent in Rhipicephalus microplus from Southwest and South-Central China. Ticks Tick Borne Dis, 2022a;13(2):101884; doi: 10.1016/j.ttbdis.2021.101884
26.
LuM, TianJ, ZhaoH, et al.Molecular survey of vector-borne pathogens in ticks, Sheep Keds, and domestic animals from Ngawa, Southwest China. Pathogens, 2022c;11(5):606; doi: 10.3390/pathogens11050606
27.
LuoJ, RenQ, LiuW, et al.Micropathogen community identification in ticks (Acari: Ixodidae) using third-generation sequencing. Int J Parasitol Parasites Wildl, 2021; 15:238–248; doi: 10.1016/j.ijppaw.2021.06.003
28.
MadariagaMG, RezaiK, TrenholmeGM, WeinsteinRA. Q fever: A biological weapon in your backyard. Lancet Infect Dis, 2003; 3(11):709–721; doi: 10.1016/s1473-3099(03)00804-1
29.
Mohabati MobarezA, BaseriN, KhaliliM, et al.Genotyping and phylogenetic analysis of Coxiella burnetii in domestic ruminant and clinical samples in Iran: Insights into Q fever epidemiology. Sci Rep, 2023; 13(1):20374; doi: 10.1038/s41598-023-47920-0
30.
NiJ, LinH, XuX, et al.Coxiella burnetii is widespread in ticks (Ixodidae) in the Xinjiang areas of China. BMC Vet Res, 2020; 16(1):317; doi: 10.1186/s12917-020-02538-6
31.
OhlopkovaOV, YakovlevSA, EmmanuelK, et al.Epidemiology of zoonotic Coxiella burnetii in The Republic of Guinea. Microorganisms, 2023; 11(6):1433; doi: 10.3390/microorganisms11061433
32.
RahravaniM, MoravedjiM, MostafaviE, et al.The epidemiological survey of Coxiella burnetii in small ruminants and their ticks in western Iran. BMC Vet Res, 2022; 18(1):292; doi: 10.1186/s12917-022-03396-0
33.
RamatlaT, KhumaloZTH, MatshotshiA, et al.Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa. Vet Med Sci, 2023; 9(5):2185–2191; doi: 10.1002/vms3.1192
34.
RooneyT, FèvreEM, VillingerJ, et al.Coxiella burnetii serostatus in dromedary camels (Camelus dromedarius) is associated with the presence of C. burnetii DNA in attached ticks in Laikipia County, Kenya. Zoonoses Public Health, 2024; 71(5):503–514; doi: 10.1111/zph.13127
35.
SeoMG, LeeSH, VanBikD, et al.Detection and genotyping of Coxiella burnetii and Coxiella-like bacteria in horses in South Kore. PLoS One, 2016; 11(5):e0156710; doi: 10.1371/journal.pone.0156710
ShiQ, SongFL, YangY, et al.Epidemiological and molecular study on tick-borne pathogens in Argun Port Area Near the Chinese-Russian Border. Vector Borne Zoonotic Dis, 2023; 23(9):447–457; doi: 10.1089/vbz.2022.0061
38.
SmithTA, DriscollT, GillespieJJ, RaghavanR. A Coxiella-like endosymbiont is a potential vitamin source for the Lone Star tick. Genome Biol Evol, 2015; 7(3):831–838; doi: 10.1093/gbe/evv016
39.
TanCK, OwensL. Infectivity, transmission and 16S rRNA sequencing of a rickettsia, Coxiella cheraxi sp. nov., from the freshwater crayfish Cherax quadricarinatus. Dis Aquat Organ, 2000; 41(2):115–122; doi: 10.3354/dao041115
40.
TanTS, Hernandez-JoverM, HayesLM, et al.Identifying scenarios and risk factors for Q fever outbreaks using qualitative analysis of expert opinion. Zoonoses Public Health, 2022; 69(4):344–358; doi: 10.1111/zph.12923
41.
ZhangB, WangX, Aguli NurlandR, et al.Investigation of tick-borne bacterial microorganisms in Haemaphysalis ticks from Hebei, Shandong, and Qinghai provinces, China. Ticks Tick Borne Dis, 2024; 15(2):102290; doi: 10.1016/j.ttbdis.2023.102290
42.
ZhangCM, LiNX, ZhangTT, et al.Endosymbiont CLS-HI plays a role in reproduction and development of Haemaphysalis longicornis. Exp Appl Acarol, 2017; 73(3–4):429–438; doi: 10.1007/s10493-017-0194-y
