OBJECTIVE: This cross-sectional study investigated the correlation between diacron reactive oxygen metabolites (d-ROMs) and high-sensitivity C-reactive protein (hs-CRP) in subjects with or without metabolic syndrome. METHODS: Cardiometabolic risk factors, d-ROMs and hs-CRP were determined in 457 women: 123 with metabolic syndrome and 334 without metabolic syndrome. The correlation between d-ROMs and hs-CRP levels was compared between the two groups. RESULTS: The group with metabolic syndrome had significantly higher d-ROMs and hs-CRP levels than the group without metabolic syndrome. While the d-ROMs level was significantly and positively correlated with the hs-CRP level in both groups, the correlation level between the two groups was significantly different. Multiple linear regression analysis adjusted for other cardiometabolic risk factors also showed significant positive correlation between d-ROMs and hs-CRP levels in both groups. CONCLUSION: Subjects with metabolic syndrome may have a closer relationship between inflammation and oxidative stress than subjects without metabolic syndrome, possibly reflecting their increased predisposition to atherosclerosis. Further studies are necessary to confirm the observed relationship.
Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults: Executive Summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA2001; 285: 2486–2497.
2.
BruceKDByrneCD: The metabolic syndrome: common origins of a multifactorial disorder. Postgrad Med J2009; 85: 614–621.
3.
HuangALVitaJA: Effects of systemic inflammation on endothelium-dependent vasodilation. Trends Cardiovasc Med2006; 16: 15–20.
Van GuilderGPHoetzerGLGreinerJJ: Influence of metabolic syndrome on biomarkers of oxidative stress and inflammation in obese adults. Obesity2006; 14: 2127–2131.
8.
SeetRCLeeCYLimEC: Markers of oxidative damage are not elevated in otherwise healthy individuals with the metabolic syndrome. Diabetes Care2010; 33: 1140–1142.
9.
StephensJWKhanolkarMPBainSC: The biological relevance and measurement of plasma markers of oxidative stress in diabetes and cardiovascular disease. Atherosclerosis2009; 202: 321–329.
10.
AlbertiABologniniLMacciantelliD: The radical cation of N,N-diethyl-para-phenylendiamine: A possible indicator of oxidative stress in biological samples. Research on Chemical Intermediates2000; 26: 253–267.
11.
IameleLFiocchiRVernocchiA: Evaluation of an automated spectrophotometric assay for reactive oxygen metabolites in serum. Clin Chem Lab Med2002; 40: 673–676.
12.
VassalleC: An easy and reliable automated method to estimate oxidative stress in the clinical setting. Methods Mol Biol2008; 477: 31–39.
13.
KamezakiFYamashitaKKubaraT: Derivatives of reactive oxygen metabolites correlates with high-sensitivity C-reactive protein. J Atheroscler Thromb2008; 15: 206–212.
14.
SakaneNFujiwaraSSanoY: Oxidative stress, inflammation, and atherosclerotic changes in retinal arteries in the Japanese population; results from the Mima study. Endocr J2008; 55: 485–488.
15.
HiroseHKawabeHKomiyaN: Relations between serum reactive oxygen metabolites (ROMs) and various inflammatory and metabolic parameters in a Japanese population. J Atheroscler Thromb2009; 16: 77–82.
16.
SugiuraTDohiYTakaseH: Increased reactive oxygen metabolites is associated with cardiovascular risk factors and vascular endothelial damage in middle-aged Japanese subjects. Vasc Health Risk Manag2011; 7: 475–482.
17.
Examination Committee of Criteria for ‘Obesity Disease’ in Japan, Japan Society for the Study of Obesity: New criteria for ‘obesity disease’ in Japan. Circ J2002; 66: 987–992.
18.
VincentHKTaylorAG: Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes2006; 30: 400–418.
19.
FurukawaSFujitaTShimabukuroM: Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest2004; 114: 1752–1761.
20.
BastardJPMaachiMLagathuC: Recent advances in the relationship between obesity, inflammation, and insulin resistance. Eur Cytokine Netw2006; 17: 4–12.
21.
GalicSOakhillJSSteinbergGR: Adipose tissue as an endocrine organ. Mol Cell Endocrinol2010; 316: 129–139.
22.
NakazonoKWatanabeNMatsunoK: Does superoxide underlie the pathogenesis of hypertension?Proc Natl Acad Sci U S A1991; 88: 10045–10048.
23.
MeyersMRGokceN: Endothelial dysfunction in obesity: etiological role in atherosclerosis. Curr Opin Endocrinol Diabetes Obes2007; 14: 365–369.
24.
FukuchiYMiuraYNabenoY: Immunohistochemical detection of oxidative stress biomarkers, dityrosine and Nɛ-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb2008; 15: 185–192.
25.
YasojimaKSchwabCMcGeerEG: Generation of C-reactive protein and complement components in atherosclerotic plaques. Am J Pathol2001; 158: 1039–1051.
26.
KobayashiSInoueNOhashiY: Interaction of oxidative stress and inflammatory response in coronary plaque instability: important role of C-reactive protein. Arterioscler Thromb Vasc Biol2003; 23: 1398–1404.
27.
DevarajSDasuMRSinghU: C-reactive protein stimulates superoxide anion release and tissue factor activity in vivo. Atherosclerosis2009; 203: 67–74.
28.
VîrgoliciBMohoraMStoianI: A comparative oxidative stress study—obesity with and without diabetes mellitus. Rom J Intern Med2005; 43: 261–268.
29.
HoekstraTGeleijnseJMSchoutenEG: Relationship of C-reactive protein with components of the metabolic syndrome in normal-weight and overweight elderly. Nutr Metab Cardiovasc Dis2005; 15: 270–278.
