Relationship between Cellular Apoptosis and the Expression of p75 Neurotrophin Receptor and Tyrosine Kinase A Receptor in Tissue Surrounding Haematoma in Intracerebral Haemorrhage
Free accessResearch articleFirst published online February, 2011
Relationship between Cellular Apoptosis and the Expression of p75 Neurotrophin Receptor and Tyrosine Kinase A Receptor in Tissue Surrounding Haematoma in Intracerebral Haemorrhage
Cellular apoptosis and the expression of p75 neurotrophin receptor (p75NTR) and tyrosine kinase A receptor (TrkA) were investigated in the tissues surrounding haematoma in patients with intracerebral haemorrhage. Specimens of tissue from near the haematoma (haemorrhagic samples) and tissue from a distant site (control samples) were collected from 14 patients with basal ganglia haemorrhage undergoing surgical intervention. Cellular apoptosis was determined by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling (TUNEL), and p75NTR TrkA location, protein and gene expression were studied using immunohistochemistry, Western blot and real-time polymerase chain reaction, respectively. The percentage of apoptotic cells and expression of p75NTR, but not of TrkA, were significantly higher in the haemorrhagic samples than in the control samples. There was a positive correlation between the percentage of TUNEL-positive cells and the percentage of p75NTR-positive cells. These results suggest that the p75NTR-dependent signal transduction pathway plays an important role in apoptosis after intracerebral haemorrhage.
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