Traumatic brain injury is often associated with acute spinal cord injury (ASCI). Insulin and chondroitinase ABC (ChABC) are both therapeutically effective, but the combined therapeutic effect of insulin and ChABC is still not clear. A combination of insulin and ChABC were used to treat a rat model of ASCI. This combination therapy prevented neuronal cell death by improving motor function, increasing cell growth and inhibiting cell apoptosis in ASCI rats. Expression of growth-associated protein 43, a marker of axonal re-growth, increased after combined treatment with insulin and ChABC. These results may provide a basis for a future method of treating ASCI.
De WinterFOudegaMLankhorstAJ: Injury-induced class 3 semaphorin expression in the rat spinal cord. Exp Neurol2002; 175: 61–75.
5.
ProperziFCarulliDAsherRA: Chondroitin 6-sulphate synthesis is up-regulated in injured CNS, induced by injury-related cytokines and enhanced in axon-growth inhibitory glia. Eur J Neurosci2005; 21: 378–390.
6.
RouleauPUngRVLapointeNP: Hormonal and immunological changes in mice after spinal cord injury. J Neurotrauma2007; 24: 367–378.
7.
BrangeJLangkjoerL: Insulin structure and stability. Pharm Biotechnol1993; 5: 315–350.
8.
CostacouTMayer-DavisEJ: Nutrition and prevention of type 2 diabetes. Annu Rev Nutr2003; 23: 147–170.
9.
ColbergSR: Physical activity, insulin action, and diabetes prevention and control. Curr Diabetes Rev2007; 3: 176–184.
10.
ZhaoYXiaoZGaoY: Insulin rescues ES cell-derived neural progenitor cells from apoptosis by differential regulation of Akt and ERK pathways. Neurosci Lett2007; 429: 49–54.
11.
WuXHYangSHDuanDY: Anti-apoptotic effect of insulin in the control of cell death and neurologic deficit after acute spinal cord injury in rats. J Neurotrauma2007; 24: 1502–1512.
12.
MorgensternDAAsherRAFawcettJW: Chondroitin sulphate proteoglycans in the CNS injury response. Prog Brain Res2002; 137: 313–332.
13.
SnowDMLemmonVCarrinoDA: Sulfated proteoglycans in astroglial barriers inhibit neurite outgrowth in vitro. Exp Neurol1990; 109: 111–130.
14.
BarrittAWDaviesMMarchandF: Chondroitinase ABC promotes sprouting of intact and injured spinal systems after spinal cord injury. J Neurosci2006; 26: 10856–10867.
IkegamiTNakamuraMYamaneJ: Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury. Eur J Neurosci2005; 22: 3036–3046.
17.
YickLWCheungPTSoKF: Axonal regeneration of Clarke's neurons beyond the spinal cord injury scar after treatment with chondroitinase ABC. Exp Neurol2003; 182: 160–168.
18.
CaggianoAOZimberMPGangulyA: Chondroitinase ABCI improves locomotion and bladder function following contusion injury of the rat spinal cord. J Neurotrauma2005; 22: 226–239.
19.
HouleJDTomVJMayesD: Combining an autologous peripheral nervous system “bridge” and matrix modification by chondroitinase allows robust, functional regeneration beyond a hemisection lesion of the adult rat spinal cord. J Neurosci2006; 26: 7405–7415.
20.
FouadKSchnellLBungeMB: Combining Schwann cell bridges and olfactory-ensheathing glia grafts with chondroitinase promotes locomotor recovery after complete transection of the spinal cord. J Neurosci2005; 25: 1169–1178.
21.
AartsLHSchotmanPVerhaagenJ: The role of the neural growth associated protein B-50/GAP-43 in morphogenesis. Adv Exp Med Biol1998; 446: 85–106.
22.
LiGLFarooqueMHoltzA: Increased expression of growth-associated protein 43 immunoreactivity in axons following compression trauma to rat spinal cord. Acta Neuropathol1996; 92: 19–26.
23.
OestreicherABDe GraanPNGispenWH: B-50, the growth associated protein-43: Modulation of cell morphology and communication in the nervous system. Prog Neurobiol1997; 53: 627–686.
24.
HuangWCKuoWCCherngJH: Chondroitinase ABC promotes axonal re-growth and behavior recovery in spinal cord injury. Biochem Biophys Res Commun2006; 349: 963–968.
25.
TencerAFFergusonRLAllenBLJr: A biomechanical study of thoracolumbar spinal fractures with bone in the canal. Part II. The effect of flexion angulation, distraction, and shortening of the motion segment. Spine1985; 10: 586–589.
26.
BassoDMBeattieMSBresnahanJC: A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma1995; 12: 1–21.
27.
WennerPO'DonovanMJ: Identification of an interneuronal population that mediates recurrent inhibition of motoneurons in the developing chick spinal cord. J Neurosci1999; 19: 7557–7567.
28.
NihalMAhmadNMukhtarH: Anti-proliferative and proapoptotic effects of (–)-epigallocatechin-3-gallate on human melanoma: Possible implictions for the chemoprevention of melanoma. Int J Cancer2004; 114: 513–521.
29.
ChenCHWangSMYangSH: Role of Thy-1 in vivo and in vitro neural development and regeneration of dorsal root ganglionic neurons. J Cell Biochem2005; 94: 684–694.
30.
HsuJYXuXM: Early profiles of axonal growth and astroglial response after spinal cord hemisection and implantation of Schwann cell-seeded guidance channels in adult rats. J Neurosci Res2005; 82: 472–483.
31.
TomVJHouléJD: Intraspinal microinjection of chondroitinase ABC following injury promotes axonal regeneration out of a peripheral nerve graft bridge. Exp Neurol2008; 211: 315–319.
32.
ChenHKohnoKGongQ: Conditional ablation of mature olfactory sensory neurons mediated by diphtheria toxin receptor. J Neurocytol2005; 34: 37–47.
