The cellular fatty acid profiles of 67 strains belonging to three different species of the genus Mycobacterium were determined by gas chromatography of the fatty acid methyl esters, using the MIDI Sherlock® Microbial Identification System (MIS). The species M. tuberculosis, M. xenopi and M. avium complex were clearly distinguishable and could be identified based on the presence and concentrations of 12 fatty acids: 14:0, 15:0, 16:1ω7c, 16:1ω6c, 16:0, 17:0, 18:2ω6,9c, 18:1ω9c, 18:0, 10Me-18:0 tuberculostearic acid, alcohol and cyclopropane. Fatty acid analysis showed that there is great homogeneity within and heterogeneity between Mycobacterium species. Thus the MIS is an accurate, efficient and relatively rapid method for the identification of mycobacteria.
HoasDW: Mycobacterial diseases. In: Principles and Practice of Infectious Diseases (MandellGLBennettJEDolinR, eds), 5th edn.Philadelphia: Churchill Livingstone, 2000; pp2576–2608.
2.
KonemanEWAllenSDJandaWMSchreckenbergerPCWinnWCJr: Color Atlas and Textbook of Diagnostic Microbiology, 5th edn.Philadelphia: Lippincott, 1997; pp893–938.
3.
KourbetiISMaslowMJ: Nontuberculous mycobacterial infections of the lung. Curr Infect Dis Rep2000; 2: 193–200.
4.
ArteroJMHerreraEAValeroPLLuengoF Martin: Comparative study of 2 rapid techniques (DNA hybridization and chromatography) for the identification of Mycobacterium tuberculosis complex isolated from clinical specimens (in Spanish). Enferm Infecc Microbiol Clin1990; 8: 505–507.
5.
LuquinMAusinaVCalahorraF LopezBeldaFBarceloM GarciaCelmaC: Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria. J Clin Microbiol1991; 29: 120–130.
6.
PinsiGSignoriniCGelmiMGarrafaEAlbertiniATuranoA: Gas chromatographic assay of cellular fatty acids and alcohols for the identification of Mycobacterium species. New Microbiol1999; 22: 151–154.
7.
TonjumTWeltyDBJantzenESmallPL: Differentiation of Mycobacterium ulcerans, M. marinum, and M. haemophilum: mapping of their relationships to M. tuberculosis by fatty acid profile analysis, DNA-DNA hybridization, and 16s rRNA gene sequence analysis. J Clin Microbiol1998; 36: 918–925.
8.
YassinAFBrzezinkaHSchaalKP: Cellular fatty acid methyl ester profiles as a tool in the differentiation of members of the genus Mycobacterium. Zentralbl Bakteriol1993; 279: 316–329.
9.
ZerbiniECardosoMMSequeiraMDSantiniMNTaherHLarpinD: Utility of gas chromatography for the identification of mycobacterial species (in Spanish). Medicina (B Aires)1999; 59: 453–458.
10.
ZhaiBZhangLYanX: Comparative experiment of gas chromatography determination of Mycobacterium bacillus (in Chinese). Zhonghua Jie He He Hu Xi Za Zhi1995; 18: 344–347.
11.
ZhangYZhuangYLiuZRuanJ: Identification of twenty-eight species of mycobacteria with their cellular fatty acids by capillary gas chromatography (in Chinese). Wei Sheng Wu Xue Bao1991; 31: 187–197.
12.
ChouSChedorePHaddadAPaulNRKasatiyaS: Direct identification of Mycobacterium species in Bactec 7H12B medium by gas-liquid chromatography. J Clin Microbiol1996; 34: 1317–1320.
13.
ChouSChedorePKasatiyaS: Use of gas chromatographic fatty acid and mycolic acid cleavage product determination to differentiate among Mycobacterium genavense, Mycobacterium fortuitum, Mycobacterium simiae, and Mycobacterium tuberculosis. J Clin Microbiol1998; 36: 577–579.
14.
Garcia-BarceloMLuquinMBeldaFAusinaV: Gas chromatographic whole-cell fatty acid analysis as an aid for the identification of mixed mycobacterial cultures. J Chromatogr1993; 617: 299–303.
15.
CuervoLM MederosAlvarezERosadoACorreaMTReyesMValdiviaJA: Analysis of fatty acids from stains of Mycobacterium habana and Mycobacterium simiae (in Spanish). Rev Cubana Med Trop1999; 51: 106–115.
16.
PinsiGSignoriniCGelmiMMancaNTuranoA: Identification of Mycobacterium xenopi by gas chromatography. New Microbiol2001; 24: 91–94.
17.
RobertsGDBottgerECStockmanL: Methods for the rapid identification of mycobacterial species. Clin Lab Med1996; 16: 603–615.
JantzenETangenTEngJ: Gas chromatography of mycobacterial fatty acids and alcohols: diagnostic applications. APMIS1989; 97: 1037–1045.
20.
TengLJLiawSJHsuehPRFanJHLuhKTHaSW: Constitutive fatty acid and enzyme profiles of Mycobacterium species. J Formos Med Assoc1997; 96: 336–345.
21.
AlugupalliSLarssonL: Secondary fatty alcohols of M. xenopi. J Gen Microbiol1992; 138: 2499–2502.
22.
MullerKSchmidENKroppenstedtRM: Improved identification of mycobacteria by using the microbial identification system in combination with additional trimethylsulfonium hydroxide pyrolysis. J Clin Microbiol1998; 36: 2477–2480.
