Influence of solvent on the yield and chemical composition of liquid products of hydrothermal liquefaction of Arthrospira platensis as revealed by Fourier transform ion cyclotron resonance mass spectrometry

Abstract

Yields of liquid products (bio-oil and residual organics in aqueous solution) of hydrothermal liquefaction (at 300℃ and 60 min) of own cultivated Arthrospira platensis using different solvents have been determined. Nonpolar hexane, moderately polar dichloromethane, and relatively more polar acetonitrile have been used. High-resolution mass spectrometry based on linear quadrupole ion trap and Fourier transform mass spectrometer (LTQ FT) has been used for liquid sample characterization. Concentration of bio-oil in a unit of solvent volume after solvent extraction took the following arrangement: acetonitrile > dichloromethane > hexane. Concentration of residual organics in a unit of aqueous solution after solvent extraction took the following arrangement: hexane >dichloromethane > acetonitrile. Yield of total organics was arranged as follows: hexane > dichloromethane > acetonitrile. Content of carbon in bio-oil was increased and the content of oxygen was decreased with the increasing of solvent polarity. From mass spectrometric analysis it was established that in the positive electrospray ionization (ESI) mode the compounds containing two nitrogen atoms dominate and the considerable portion of the compounds containing single nitrogen atom are also presented for both bio-oil and residual organics samples. In the negative ESI mode the compounds containing four oxygen atoms dominated except bio-oil obtained using hexane where the compounds containing two oxygen atoms dominated. Bio-oil fraction had highly saturated compounds with low double bond equivalent values while the compounds of the residual organics fraction had large double bond equivalent values.

Keywords

Hydrothermal liquefaction microalgae bio-oil solvent extraction chemical composition mass spectrometry Fourier transform ion cyclotron resonance high resolution

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