Investigations on the diversity of species of filamentous fungi found in the premises of five medical institutions: two hospitals, the museum of heart surgery, the centre of mental health, and a newly established medicine packing factory, have been carried out. A total of 155 species ascribed to 41 fungal genera were isolated and identified, of which species from the genera of Penicillium, Aspergillus, Acremonium, Paecilomyces, Mucor and Cladosporium made up the vast majority. A number of airborne fungi were also found in the same premises as well. Among the fungi found were several identified as allergenic and opportunistically pathogenic species. The potential health risk for the patients and staff posed by the fungi isolated is discussed.
2 Rotstein C , Cummings KM , Tidings J , Killion K , Powell E , Gustafson TL , Higby D : An outbreak of invasive aspergillosis among allogeneic bone marrow transplants: a case-control study . Infect Control1985;6: 347–355 .
3.
3 Macher JM , Streifel AJ , Vesley D : Problem Buildings, Laboratories and Hospitals; in Burge HA (ed.): Bioaerosols. London, Lewis Publishers , 1995.
4.
4 Sessa A , Meroni M , Battini G , Pitingolo F , Giordano F , Marks M , Casella P : Nosocomial outbreak of Aspergillus fumigatus infection among patients in a renal unit . Nephrol Dial Transplant1996;11: 1322–1324 .
5.
5 Kontoyiannis DP , Bodey GP : Invasive aspergillosis in 2002: an update . Eur J Clin Microbiol Infect Dis2002;21: 161–172 .
6.
6 Kennedy MJ , Sigler L : Aspergillus, Fusarium and other opportunistic moniliaceous fungi; in Murray PR , Baron EJ , Pfaller MA , Tenover FC , Yolken RH (eds): Manual of Clinical Microbiology, ed. 3. Washington, ASM Press , 1995, pp. 765–790.
7.
7 Rinaldi MG : Aspergillosis; in Balows A , Hausler WJ , Ohashi M , Turano A (eds): Laboratory Diagnosis of Infectious Diseases. Principles and Practice. Vol. 1. Bacterial, Mycotic and Parasitic Diseases. New York, Springer-Verlag , 1988, pp. 559–572.
8.
8 Goodwin SD , Fiedler-Kelly J , Grasela TH , Schell WA , Perfect JR : A nation-wide survey of clinical laboratory methodologies for fungal infections . J Med Vet Mycol1992;30: 153–160 .
9.
9 Walsh TI , Groll AH : Emerging fungal pathogens: evolving challenges to immunocompromised patients for the twenty-first century . Transpl Infect Dis1999;1: 247–261 .
10.
10 Weems JJ , Davis BJ , Tablan OC , Kaufman L , Martone WJ : Construction activity: an independent risk factor for invasive aspergillosis and zygomycosis in patients with hematologic malignancy . Infect Control1987;8: 71–75 .
11.
11 Iwen PC , Davis JC , Reed EC , Winfield BA , Hinrichs SH : Airborne fungal spore monitoring in a protective environment during hospital construction, and correlation with an outbreak of invasive aspergillosis . Infect Control Hosp Epidemiol1994;15: 303–306 .
12.
12 Lentino JR , Rosenkranz MA , Michaels JA , Kurup VP , Rose HD , Rytel MW : Nosocomial aspergillosis: a retrospective review of airborne disease secondary to road construction and contaminated air conditioners . Am J Epidemiol1982;116: 430–437 .
13.
13 Construction-related nosocomial infections in patients in health care facilities. Canada Communicable Disease Control 2001; 27s2 (http://www.hc-sc.gc.ca/pphb-dgspsp/publicat/ccdr-rmtc/01vol27/27s2/index.html).
14.
14 Krasinski K , Holzman RS , Hanna B , Greco MA , Graff M , Bhogal M : Nosocomial fungal infection during hospital renovation . Infect Control1985;6: 278–282 .
15.
15 Perraud M , Piens MA , Nicoloyannis N , Girard P , Sepetjan M , Garin JP : Invasive nosocomial pulmonary aspergillosis: risk factors and hospital building works . Epidemiol Infect1987;99: 407–412 .
16.
16 Arnow PM , Sadigh M , Costas C , Weil D , Chudy R : Endemic and epidemic aspergillosis associated with in-hospital replication of Aspergillus organisms . J Infect Dis1991;164: 998–1002 .
17.
17 Humphreys H , Johnson EM , Warnock DW , Willatts SM , Winter RI , Speller DC : An outbreak of aspergillosis in a general ITU . J Hosp Infect1991;18: 167–177 .
18.
18 Fotedar R , Shriniwas UB , Verma A : Cockroaches (Blattella germanica) as carriers of micro-organisms of medical importance in hospitals . Epidemiol Infect1991;107: 181–187 .
19.
19 Grubliauskas L , Masteikienė R , Pečiulis J , Valikonytė V : Food microbiology. Vilnius, Mokslas , 1983 (in Lithuanian).
20.
20 Wanner HU , Verhoeff A , Colombi A , Flannigan B , Gravesen S , Mouilleseaux A , Nevalainen A , Papadakis J , Seidel K : Biological Particles in Indoor Environments. Report No 12. Brussels, Commission of the European Community , 1993.
21.
21 Raper KB , Fennel J , Austwick PKC : The genus Aspergillus. Baltimore, MD, Williams & Wilkins Co. , 1965.
23 Ellis MB : More Dematiaceous Hyphomycetes. London, Kew: Commonwealth Mycological Institute , 1976.
24.
24 Domsch KH , Gams W , Anderson TH : Compendium of Soil Fungi. Vol. 1. London, Academic Press , 1980.
25.
25 Ramirez C : Manual and Atlas of the Penicillia. Amsterdam, Elsevier , 1982.
26.
26 Lugauskas A , Mikulskienė A , Šliaužienė D : Catalogue of the Fungi Deteriorating Polymeric Materials. Moscow, Nauka , 1987 (in Russian).
27.
27 St-Germain G , Summerbell R : Identifying Filamentous Fungi. A Clinical Laboratory Handbook. Belmont, CA, Star Pub Co , 1996.
28.
28 Kwon-Chung KJ , Bennett JE : Medical Mycology. Philadelphia–London, Lea & Febiger , 1992.
29.
29 Anandi V , John TJ , Walter A , Shastry JCM , Lalitha MK , Padhye AA , Ajello L , Chandler FW : Cerebral phaeohyphomycosis caused by Chaetomium globosum in a renal transplant recipient . J Clin Microbiol1989;27: 2226–2229 .
30.
30 Stiller MJ , Rosenthal S , Summerbell RC , Pollack J : Onychomycosis of the toenails caused by Chaetomium globosum . J Am Acad Dermatol1992;26: 775–776 .
31.
31 Zabawski J , Baran E : Description of more frequently occurring pathogenic and opportunistic fungi from the subclasses of Zygomyco-tina, Ascomycotina and Deuteromycotina; in Barrn E (ed.): Study of Medical Mycology. Wroclaw, Volumed , 1998, pp. 37–254. (in Polish).
32.
32 Lugauskas A , PaŠkevičius A , Repečkienė J : Pathogenic and toxic micro-organisms in the human environment. Vilnius, Aldorija , 2002. (in Lithuanian).
33.
33 Singh SM , Scharma S , Chatterjee PK : Clinical and experimental mycotic keratitis caused by Aspergillus terreus and the effect of subconjuctival oxiconazole treatment in the animal model . Mycopathologia1990;112: 127–137 .
34.
34 Garrett MH , Rayment PR , Hooper MA , Abramson MJ , Hooper BM : Indoor airborne fungal spores, house dampness and associations with environmental factors and respiratory health in children . Clin Exp Allergy1998;28: 459–467 .
35.
35 Kurup VP , Banerjee B , Hemmann S , Greenberger PA , Blaser K , Crameri R : Selected recombinant Aspergillus fumigatus allergens bind specifically to IgE in ABPA . Clin Exp Allergy2000;30: 988–993 .
36.
36 Rand TG , Mahoney M , White K , Oulton M : Micro-anatomical changes in alveolar type II cells in juvenile mice intratracheally exposed to Stachybotrys chartarum spores and toxin . Toxicol Sci2002;65: 239–245 .
37.
37 Madelin TM , Madelin MF : Biological analysis of fungi and associated molds; in Cox CS , Wathes CM (eds): Bioaerosols Handbook. Boca Raton, Lewis Publishers, Inc. , 1995, pp. 361–386.
38.
38 Reponen T : Aerodynamic diameters and respiratory deposition estimates of viable fungal spores in mould problem homes . Aerosol Science and Technology1995;22: 11–23 .
39.
39 Dutkiewicz J : Bacteria and Fungi in Organic Dust as Potential Health Hazards . Ann Agric Environ Med1997;4: 11–16 .
