Abstract
For purposes of this study, micro-adaptations of established fluorometric and spectrophotometric methods were developed for quantitative measurement in microtome sections of tissue of NADPH- and NADH-dependent dichlorophenolindophenol reductases (diaphorases) and quinone reductases, and also of NADPH and NADH oxidases and NADPH-NAD transhydrogenase. Correlations of the enzyme activities with the histology of the rat adrenal gland were made, both in glands from animals injected with ACTH and from control animals given saline injections. The functional significance of the results was considered in relation to electron transport and hormone biosynthesis in the organ.