Experimental investigation of proangiogenic properties of sacubitril on a chorio allantoic membrane model

Abstract

Background

Sacubitril is an important and effective agent for cardiovascular remodeling. This study aims to evaluate its proangiogenic effects in an experimental model.

Methods

The chorioallantoic membrane (CAM) model was created for the investigation of the proangiogenic potential of sacubitril with different therapeutic doses (10⁻⁷ M, 10⁻⁶ M, 10⁻⁵ M) and compared with drug-free pellet group (sham) and normal morphology (control) of chick embryo development in drug-free chick embryos. The developing embryo's vascularity and the pellets’ effect were evaluated under a stereoscopic microscope. The density of vascular shoots and newly formed vascular nodules were not recorded.

Results

There was no significant difference between the control and drug-free pellet groups (12.4 ± 2.8 vs. 14.1 ± 1.3 junctions per ROI, p = 0.48). The incremental angiogenic properties were detected in drug groups as follows: 15.3 ± 3.8 per ROI in Group I (10⁻⁷ M concentration); 21.6 ± 5.4 per ROI in Group II (10⁻⁶ M concentration); 22.9 ± 8.1 per ROI in Group III (10⁻⁵ M concentration) (p < 0.001).

Conclusion

Our findings support that sacubitril provokes angiogenesis in a dose-dependent manner. Investigating these properties can be useful for understanding further effects of this agent in other cardiovascular diseases. Therapeutic angiogenesis is important for ameliorating the results.