The Copenhagen Burnout Inventory was developed to address limitations in the MBI-HSS, the predominant burnout assessment tool for healthcare professionals. This study evaluated the psychometric properties of the CBI in a sample of 510 Colombian healthcare workers. Using a cross-sectional design, reliability, confirmatory factor analysis, and item response theory analyses were conducted. Results showed high internal consistency (ω = 0.89–0.92). The original structure was not fully confirmed, as item 13 exhibited low factor loading and high measurement error. After removing it, the model achieved adequate fit (CFI and TLI ⩾ 0.95; RMSEA ⩽ 0.05). IRT analyses revealed appropriate item discrimination and ascending response thresholds, with the tool better differentiating individuals with low and high burnout levels. Correlations between subscales reflected associations among dimensions. The CBI is a valid and reliable tool for assessing burnout in Colombian healthcare professionals but removing item 13 is recommended to optimize its measurement properties.
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