Abstract
It was demonstrated that acridine orange, analogous to cetyl pyridinium chloride, precipitates acid mucopolysaccharides, and that the precipitates can be redissolved in solutions of graded sodium chloride concentration. The established fractional solution technique for acid mucopolysaccharide complexes with cetyl pyridinium chloride has been applied to histochemical staining by replacing the cetyl pyridinium chloride with acridine orange. The staining method described differentiates between the presence of hyaluronic acid, chondroitin sulfuric acid and heparin.