Acute Freeze-Dried Mango Consumption With a High-Fat Meal has Minimal Effects on Postprandial Metabolism,Inflammation and Antioxidant Enzymes

Abstract

Objective:

Postprandial fluxes in oxidative stress, inflammation, glucose, and lipids, particularly after a high-fat meal (HFM), have been implicated in the development of cardiovascular disease (CVD). The aim of this study is to determine whether acute freeze-dried mango consumption modulates the postprandial response to an HFM. We hypothesized that the addition of mango, which is a rich source of many bioactive components, to an HFM would lower postprandial triglycerides, glucose, and inflammation, and increase antioxidant enzymes, compared to a standard HFM alone.

Methods:

In a randomized cross-over study, 24 healthy adult males (18-25 years old) consumed a typical American breakfast (670 kcal; 58% fat) with or without the freeze-dried mango pulp (50 g). Lipids, glucose, antioxidant enzymes, and inflammatory markers were assessed at baseline/fasting and 1, 2, and 4 hours after the HFM.

Results:

Addition of mango resulted in lower glucose (95.8 ± 4.4 mg/dL; P = .002) and higher high-density lipoprotein cholesterol (HDL-C; 58.4 ± 2.7 mg/dL; P = .01) 1 hour post-HFM compared to control (glucose: 104.8 ± 5.4 mg/dL; HDL-C: 55.2 ± 2.3 mg/dL), although no differences were observed in triglycerides (P = .88 for interaction). No significant meal × time interactions were detected in markers of inflammation (C-reactive protein, P = .17; interleukin-6, P = .30) or antioxidant enzymes (superoxide dismutase, P = .77; glutathione peroxidase, P = .36; catalase, P = .32) in the postprandial period.

Conclusions:

When added to an HFM, acute mango consumption had modest beneficial effects on postprandial glucose and HDL-C responses, but did not alter triglyceride, inflammatory, or antioxidant enzymes.

Keywords

Mango postprandial glucose inflammation antioxidant enzymes

Introduction

The importance of the postprandial state in the development of chronic diseases is increasingly being recognized.¹ Approximately 16 hours per day are spent in the postprandial phase and changes that occur during this state, particularly after consuming a high-fat meal (HFM), can have a significant impact on the development of chronic diseases, such as diabetes and cardiovascular disease (CVD).^1,2 Following an HFM, there is a transient increase in circulating triglycerides (TG) that lasts for several hours.³ This postprandial increase in TG is involved in atherogenesis by increasing cholesterol-dense chylomicron remnants (CMRs) that are produced when TG are removed from chylomicrons and exchanged with cholesterol from high-density lipoprotein (HDL). High-density lipoprotein cholesterol (HDL-C) is reduced as a result of this lipid exchange. This cholesterol-dense CMR is more easily oxidized and can form foam cells that contribute to the formation of atherosclerotic plaque.³

An HFM is also accompanied by an increase in oxidative stress and reactive oxygen species (ROS).^4-8 Reactive oxygen species is a normal product of metabolic processes during the postprandial response such as the oxidation of carbohydrate, fats, and proteins. Reactive oxygen species circulate throughout the body and interact with lipids, proteins, and nucleic acids, and can damage cellular components and increase risk for many chronic diseases, including CVD. Reactive oxygen species are tightly regulated under normal conditions by antioxidants in the body such as enzymes, vitamins, and phenolic compounds.⁹ Endogenous as well as dietary antioxidants scavenge the extra electrons in ROS and stabilize the molecule, thus preventing damage to the cells. However, in diseases such as CVD, the production and function of these antioxidant enzymes decreases while ROS continue to increase, resulting in an overall redox state that favors chronic oxidative stress.

In addition to oxidative stress, inflammation also plays a role in the pathogenesis of CVD.¹⁰ Interestingly, it has been demonstrated that markers of inflammation can increase in the acute period following HFM consumption.¹¹ Specifically, interleukin (IL)-6 has been found to increase in >70% of postprandial studies, approximately doubling in concentration in response to an HFM.¹² The continuous release of IL-6, and the acute phase protein, C-reactive protein (CRP), produced in the liver, can result in increased production of adhesion molecules and ultimately lead to endothelial dysfunction and increased risk of CVD.^13,14

It is well known that lipid levels, endothelial dysfunction, oxidative stress, and inflammation are linked to the dietary pattern.^2,3 Functional foods are foods which have the ability to perform tasks in the body beyond the basic functions of a nutrient. They contain phytochemicals, antioxidants, and other nutrients which have been shown to modulate lipid profile, decrease inflammatory markers, increase antioxidant enzymes, regulate the immune system, and much more.^15-18 The effects of these functional foods, particularly fruits and vegetables, on the glycemic response and postprandial oxidative stress and endothelial dysfunction have also been investigated.^19-22 One fruit of interest is mango, grown and popular to sunny, warm climates. Mangos are packed with many nutrients (beta-carotene, vitamin C, and folate) as well as phenolic compounds (chlorogenic, gallic, vanillic, and protocatechuic acids) which confer health-promoting properties.^23,24 Extracts from the stem, skin, and pulp of mango have all been tested in cell culture and animal models and have generally been shown to possess anti-inflammatory, antidiabetic, and immune-regulatory properties.^{15,16,18,25,26}

Although a few human studies have been conducted to investigate the health benefits of mango consumption, improvements in lipids, glucose, and antioxidant capacity have been reported.^25,27 There are even fewer studies that investigated the effects of mango on the postprandial response. Two pilot human studies compared the glycemic response of 25 g of mango and other tropical fruits in 10 diabetic subjects.^19,20 Roongpisuthipong et al¹⁹ found mango and banana had significantly lower glucose response curves compared to pineapple, rambutan, and durian in female diabetics. Contractor et al²⁰ found mango and sapota had a lower area under the curve (AUC) for glucose than banana in diabetic subjects. These two studies demonstrate that mango pulp, despite its sweet taste, does not cause a rapid increase in blood glucose. In addition, mango has been reported to have a moderate glycemic index (approximately 41-60).²⁸ However, the effects of mango fruit in combination with a high-fat meal on postprandial responses have not been explored.

Therefore, the purpose of this study is to investigate the effects of acute consumption of freeze-dried mango together with an HFM on postprandial metabolic, antioxidant enzymes, and inflammatory responses. We hypothesize that the addition of mango, which is a rich source of many bioactive components, to an HFM will modulate the postprandial response, resulting in lower TG, glucose, and inflammation, and greater antioxidant status, compared to a standard HFM alone.

Materials and Methods

Participants

Twenty-four healthy males (age 18-25 years) were recruited from the campus of Oklahoma State University to participate in this study. Interested individuals were interviewed over the phone to determine if they met the inclusion and exclusion criteria. Any healthy males within the specified age range and not violating any of the exclusion criteria were included in the study. Exclusion criteria included having taken (within the last month) or currently taking any kind of dietary supplements, diagnosed with a medical condition, smoking, or consuming alcohol or illicit drugs. This study was conducted according to the guidelines in the Declaration of Helsinki and all procedures were approved by the Institutional Review Board at the Oklahoma State University.

Overall study design

In this randomized cross-over study, participants consumed an HFM alone (Control condition) and an HFM with the addition of a mango shake (Mango condition) in random order. For each trial, participants came to the study site following a 10-hour overnight fast. First, participants completed questionnaires, and anthropometric measurements were taken (described below). Next, a baseline blood draw was performed by the study phlebotomist. After the first blood draw in the first session, subjects were randomized to either the Control or Mango condition. They were given 20 minutes to consume the respective meal. The HFM was a typical high-fat American breakfast consisting of a sausage and egg biscuit with hash browns from McDonald’s (675 kcal; 19 g protein, 53 g carbohydrate, 43 g fat [16 g saturated fat]; 11% protein, 31% carbohydrate, 58% fat). For the Mango condition, a mango shake (containing 50 g freeze-dried mango powder, a cup of water, and a cup of ice blended together) was added to the meal. The 50 g serving of freeze-dried mango powder utilized in the present study contains 45.4 g carbohydrate (181.6 kcal), 2.2 g fiber, 1.8 g protein (7.2 kcal), and 0.6 g fat (5.4 kcal) yielding 194 kcal per serving (Nestle Purina Analytical Laboratories; St. Louis, MO). The dose of 50 g of freeze-dried mango is approximately 500 g or three cups of fresh mango.¹⁸ The mango pulp is a rich source of vitamin C, β-carotene, and phenolic compounds.^23,24 The HFM and mango shake were consumed simultaneously in the Mango condition. In the Control condition, participants were given water to drink during the meal. After consumption of the meal, participants were allowed to drink water ad libitum throughout the visit. Repeated blood draws were taken at 1, 2, and 4 hours after the meal was complete. After the first visit, participants completed the other condition following a 3- to 4-week washout period, where the same procedure was performed. The washout period of 3 to 4 weeks has been used in other cross-over studies^29,30 and was chosen to ensure that the participants were back to their normal routine in terms of diet and physical activity as well as to remove any residual effects of mango and the stress of blood draws. Participants were asked to follow their normal diet and physical activity patterns between the two visits.

Anthropometrics, questionnaires, and blood collection

Anthropometric measurements (height, weight, waist and hip circumference, and blood pressure), health and physical activity questionnaires, and a 24-hour dietary recall were recorded at both visits. Height was measured to the nearest centimeter using a wall-mounted stadiometer (Shorr Productions; Olney, MD), while weight was acquired to the nearest 10th of a pound using a digital scale (SECA; Chino, CA). Waist and hip measurements were taken using a flexible tape measure (to the nearest ½ inch). An automatic blood pressure monitor (Omron Healthcare; Bannockburn, IL) was used to measure blood pressure.

The Physical Activity Scale 2 (PAS 2) questionnaire³¹ was used to determine whether the participants altered their exercise habits while participating in the study. This questionnaire assessed the number of hours of light, moderate, and heavy physical activity performed for work or leisure time during a week.³¹ Dietary intake was assessed using 24-hour dietary recall, which was analyzed using the Food Processor Nutrition Analysis and Fitness software (ESHA Research; Salem, OR).

Approximately 5-mL blood samples were collected via single venipuncture in ethylenediaminetetraacetic acid (EDTA) tubes at 0, 1, 2, and 4 hours after the meal in both trials. The samples were placed on a Labquake shaker (Barnstead Thermolyne; Dubuque, IA) and allowed to mix for 5 to 10 minutes. Blood was centrifuged at 3500 r/min to obtain plasma, which was aliquoted, and stored at −80°C for later analyses.

Clinical chemistry analyses

A Biolis 24i automated clinical chemistry analyzer (Carolina Chemistry; Winston-Salem, NC) was used for the determination of plasma concentrations of alanine aminotransferase (ALT), low-density lipoprotein cholesterol (LDL-C), HDL-C, total cholesterol (Total-C), TG, high-sensitivity CRP, and glucose (all from Carolina Liquid Chemistries Corp; Brea, CA), as well as nonesterified fatty acids (NEFAs; Wako; Richmond, VA) per manufacturer’s instructions. All measurements were performed in duplicate and the inter-assay coefficient of variation were 2.7%, 2.5%, 1.8%, 2.2%, 3.0%, 3.7%, 2.7%, and 2.8% for ALT, LDL-C, HDL-C, Total-C, TG, high-sensitivity CRP, glucose, and NEFA, respectively.

Antioxidant enzymes and inflammatory marker analyses

Colorimetric kits from Cayman Chemical (Ann Arbor, MI) were used to analyze total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity. Interleukin-6 activity was determined using the sandwich enzyme-linked immunosorbent assay (ELISA) kits from R&D Systems (Minneapolis, MN). All measurements were done in duplicate and the inter-assay coefficient of variation were 3.7%, 9.9%, 7.2%, and 4.5% for SOD, CAT, GPx, and IL-6, respectively.

Statistical analyses

Statistical analyses, power calculation, and figure generation were performed using SAS software version 9.4 (SAS Institute; Cary, NC) and GraphPad Prism software version 7.03 (GraphPad Software; La Jolla, CA). Powers for detecting differences were calculated based on a range of possible decreases in plasma glucose with mango supplementation.²⁵ The mean value was assumed to be 95 mg/dL with a standard deviation of 7.5 mg/dL. These values were arrived at under the assumption that the study group contains only starting glucose values ranging from 80 to 110 mg/dL. To calculate power, or the probability of detecting a specified difference, a two-tailed significance level of .05 was used. A noncentral t distribution with the analysis of variance (ANOVA) error degrees of freedom and the noncentrality parameter of the percent decrease expressed as a fraction of the standard error of a paired comparison were used. The power for detecting a 6 unit difference in blood glucose (6.3% change) was calculated to be 80%.

Data were checked for normality via Shapiro-Wilk normality test. Potential pretrial differences in baseline participant characteristics, fasting metabolic levels, and sleep activity data leading up to each trial were assessed via paired t test. A two-way (trial × time) repeated measures ANOVA with Tukey’s adjustment for multiple comparisons was used to test for postprandial interactions or meal/group effects on markers of metabolism, inflammation, and antioxidant enzymes. A P value of less than .05 was considered statistically significant.

Results

Participant characteristics

Twenty-four healthy males participated in this cross-over study. At each visit, height, body mass, waist and hip circumference, and systolic and diastolic blood pressure were measured and no statistical differences were found (Table 1). The only significant difference in fasting metabolic markers was observed in Total-C, which was higher in the Control trial than the Mango trial (Table 1). There were no significant differences in nutrient intake (i.e. total calories, macronutrients, and fiber), sleep duration or physical activity levels before each visit (Table 2).

Table 1.

Participant characteristics and fasting metabolic levels at baseline of each trial.

Parameters	Mango (n = 24)	Control (n = 24)	P value
Age (years)	21.0 ± 1.9	21.0 ± 1.9	.91
Height (in.)	70.7 ± 0.6	70.6 ± 0.6	.26
Weight (kg)	79.3 ± 3.0	78.3 ± 3.0	.82
BMI (kg/m²)	24.6 ± 0.9	24.3 ± 0.9	.81
Waist circumference (in.)	34.6 ± 0.9	34.4 ± 0.9	.41
Hip circumference (in.)	37.2 ± 0.9	37.0 ± 0.7	.50
Systolic BP (mm Hg)	122.8 ± 1.7	121.0 ± 2.4	.26
Diastolic BP (mm Hg)	70.1 ± 1.5	69.6 ± 1.5	.68
Triglycerides (mg/dL)	75.0 ± 11.5	77.1 ± 16.2	.75
Glucose (mg/dL)	93.4 ± 1.8	96.0 ± 2.5	.17
NEFA (mEq/L)	0.44 ± 0.05	0.42 ± 0.03	.72
HDL-C (mg/dL)	58.2 ± 2.5	57.2 ± 2.3	.56
LDL-C (mg/dL)	84.9 ± 4.0	86.9 ± 5.0	.62
Total-C (mg/dL)	148.4 ± 6.2	159.3 ± 6.3	.03

Data represent mean ± SEM.

Abbreviations: BMI, body mass index; BP, blood pressure; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; NEFA, nonesterified fatty acids; Total-C, total cholesterol.

Table 2.

Nutrient intake, sleep, and physical activity (PA) data leading up to each trial.

Parameters	Mango (n = 24)	Control (n = 24)	P value
Select nutrient intake
Calories	2158 ± 148	2240 ± 161	.69
Protein (g)	89.4 ± 8.7	89.5 ± 7.8	.99
Fat (g)	85.3 ± 8.2	86.8 ± 7.2	.90
Carbohydrates (g)	261.8 ± 21.4	282.5 ± 23.7	.37
Monosaccharides (g)	3.1 ± 0.9	4.7 ± 1.5	.34
Disaccharides (g)	12.6 ± 3.5	13.2 ± 4.3	.93
Dietary fiber (g)	14.4 ± 1.5	14.8 ± 1.4	.84
Sleep and physical activity
Sleep (min/night)	431.3 ± 12.9	428.8 ± 10.9	.78
Sedentary activity (min/day)	337.9 ± 44.0	291.3 ± 42.7	.47
Standing/walking (min/days)	89.2 ± 25.3	106.0 ± 25.0	.39
Heavy work (min/day)	29.6 ± 9.0	34.6 ± 9.8	.65
Leisure—no PA (min/day)	210.0 ± 42.2	173.8 ± 28.1	.29
Leisure—light PA (min/week)	283.1 ± 48.6	213.5 ± 45.1	.29
Leisure—moderate PA (min/week)	88.8 ± 21.2	113.8 ± 25.2	.42
Leisure—strenuous PA (min/week)	174.0 ± 36.7	146.3 ± 31.5	.35

Data represent Mean ± SEM.

Leisure (no PA)—min/day watching TV, sitting quietly, reading, and listening to music or the like. Leisure (light PA)—min/week engage in light physical activity such as walking, light cleaning, raking lawn, or lightly strenuous exercise such as yoga, bowling, or similar activities. Leisure (moderate PA)—min/week engaged in gardening, carrying, loads upstairs, or moderately strenuous sport such as gymnastics, swimming, bicycling, strengths conditioning, or similar activities. Leisure (strenuous PA)—min/week engaged in strenuous sport and conditioning exercise such as running, jogging, soccer, tennis, aerobics, or similar activities.

Lipids and glucose

Fasting and postprandial metabolic values are displayed in Figure 1. Acute mango consumption in conjunction with an HFM resulted in significantly lower (P = .002) 1-hour postprandial plasma glucose (95.8 ± 4.4 mg/dL) compared to no supplementation (104.8 ± 5.4 mg/dL). However, no difference was observed in plasma glucose at any other time points after the meal. Plasma TG was elevated at every time point in the postprandial period in both groups, but there were no differences between groups. Similarly, NEFA were lower at 1 and 2 hours post-meal, but there were no group differences.

Figure 1.

Fasting and postprandial metabolic values in the Mango and Control trials. Triglycerides (top panel), glucose (middle panel), and NEFA (bottom panel) were assessed at baseline/fasting (time 0) and 1, 2, and 4 hours after the high-fat meal. Significant differences (P < .05) at specific time points reflect the results of a two-way (trial × time) repeated measures ANOVA. Error bars reflect SEM.

Cholesterol responses are displayed in Figure 2. Significant meal × time interactions were detected for HDL-C (P = .04) and Total-C (P = .03). At 1 hour post-meal, HDL-C was significantly higher (P = .01) in the Mango trial (58.4 ± 2.7 mg/dL) than in the Control trial (55.2 ± 2.3 mg/dL). Total-C was lower at baseline (P < .0001) and 2 (P = .0009) and 4 hours (P = .0007) post-meal in the Mango trial than in the Control trial.

Figure 2.

Fasting and postprandial cholesterol values in the Mango and Control trials.

Liver and inflammatory markers

Alanine aminotransferase and inflammation responses are exhibited in Figure 3. A significant meal × time interaction was determined for ALT (P = .02), but not for CRP (P = .17) or IL-6 (P = .30). Alanine aminotransferase decreased significantly in the Control trial (P = .0005) from baseline (23.8 ± 2.4 IU/L) to 1 hour post-meal (20.4 ± 1.8 IU/L), and was lower than the Mango trial (23.1 ± 2.2; P = .007). No trial- or time-based differences were detected in CRP or IL-6.

Figure 3.

Fasting and postprandial ALT and inflammation values in the Mango and Control trials.

Antioxidant enzymes

With regard to antioxidant enzymes (Figure 4), there were no statistically significant time × trial interactions for SOD (P = .77), GPx (P = .36), or CAT (P = .32). A time effect (P = .03) was detected for SOD, but post hoc analyses of specific time points revealed no significant differences (P > .05).

Figure 4.

Fasting and postprandial antioxidant enzymes in the Mango and Control trials.

Discussion

The aim of this study is to investigate whether acute consumption of freeze-dried mango pulp together with an HFM would modulate postprandial responses in healthy young men. This study demonstrated that acute mango consumption together with an HFM had modest effect on postprandial lipids and glucose, but no effect on antioxidant enzymes and inflammatory markers, compared to the HFM without mango fruit.

Glucose, lipids, and liver markers

In this study, plasma glucose was significantly decreased 1 hour postmeal after the mango supplemented HFM compared to the nonsupplemented meal. Two studies previously looked at the glucose AUC of mango compared to other fruits high in carbohydrate and found mango’s response to be significantly lower.^19,20 The lower peak in blood glucose in this study could have been due to decreased or delayed absorption from the fiber content in the mango. Kay and Holub²¹ also reported a significant decrease in plasma glucose 3 and 4 hours following 100 g blueberry supplementation after an HFM consumed by healthy adults. However, unlike this study, significant decreases in LDL-C were also observed at 3 and 4 hours after meal.²¹ Violi et al³² also found similar results in the glycemic and lipid response to that of Kay et al after extra virgin olive oil supplementation during a Mediterranean style meal. Similar to this study design and findings, Esposito et al³³ compared an HFM alone to an HFM supplemented with antioxidants and did not find any significant differences in blood lipids or glucose parameters in healthy participants. Overall, the finding in our study of lower glucose levels 1 hour after meal intake in the Mango trial points to a potentially noteworthy health benefit of freeze-dried mango pulp consumption.

Elevations in TG during the postprandial period have been proposed as an independent CVD risk factor due to the production TG-rich lipoproteins (TRLs) which are readily acted upon by lipase enzymes forming small, dense easily oxidizable lipoproteins.³⁴ This study showed that acute mango consumption did not contribute to any significant differences in postprandial TG. Chronic (3 month) intakes of mango and mangiferin supplements have been shown to significantly improve glycemic and lipid profiles in animal and human studies, respectively.^26,35 For effects on lipid profile, it was proposed that mangiferin, the main phenolic compound in mango, increased transcription of genes involved in fatty acid oxidation.³⁵ The short duration of a postprandial study is likely insufficient to detect transcriptional changes in enzymes involved in lipid metabolism. Interestingly, we did find higher HDL-C in the Mango trial compared to the Control trial 1 hour postmeal. High-density lipoprotein cholesterol is known to generally decrease in the postprandial period following HFM consumption and is typically inversely associated with TG.³⁶ The explanation for the beneficial effect of Mango on HDL-C without differences in TG is unclear, but suggests a modest protective effect of mango when consumed in conjunction with an HFM. In terms of Total-C, it is hard to determine the exact effects of mango supplementation, since there was a significant difference in baseline concentration between the Control and Mango trial. We cannot explain the difference in baseline concentrations of Total-C between the Control and Mango trial. Nonetheless, consumption of mango shake did not alter Total-C concentration during the postprandial state.

Markers of inflammation

Elevations in inflammatory markers have been reported as risk factors for CVD.^14,37 In this study, there were no significant differences in inflammatory markers after either meal. Interleukin-6 is an inflammatory marker that appears to be upregulated following a variety of stressors to the human body, including exercise and HFM intake.^37-39 Interleukin-6 stimulates the acute phase response, resulting in increased secretion of CRP from the liver, an independent risk factor for CVD.^14,40 Other studies have demonstrated IL-6 increases in response to an HFM.^7,41 Specifically, a recent systematic review found that IL-6 increased in >70% of studies assessing postprandial inflammation.¹² This review also found that the peak IL-6 response occurs ~6 hours post-HFM. In our study, it appears that IL-6 may be increasing at 4 hours postmeal, although not statistically significant. Therefore, it is possible that our postprandial period was simply not long enough in duration to sufficiently detect a change in IL-6.

Mixed results have been seen for CRP during the postprandial response.³³ The previously mentioned systematic review found that CRP did not change in 79% of previous studies.¹² Given that CRP is a downstream marker, primarily increasing in response to IL-6, it is understandable why CRP may not increase in the acute postprandial period. Previous work in an endotoxin model suggests that CRP increases ~24 hours poststimulus.¹¹ Therefore, the lack of meal-induced changes and group differences in this study are not surprising. Overall, in our study, mango did not have a noteworthy effect on the postprandial inflammatory process, as measured by IL-6 and CRP.

Antioxidant enzymes

Oxidative stress is a significant factor in the development of CVD by damaging the endothelium and oxidizing LDL.⁹ In this study, endogenous antioxidant enzymes were measured as an indirect marker of oxidative stress. However, other than a significant main effect of time (with no significant pairwise comparisons) in SOD, we did not find any postprandial changes or group differences in antioxidant enzymes. Our findings differ from the results of Cardona et al,⁴² who observed a 30% increase in SOD and significant reductions in GPx in healthy adults following an HFM. The discrepancies between results could be due to the (1) type of meal consumed; a lipid load elicited a much higher oxidative stress response when compared to a meal of all three macronutrients, (2) physical fitness; physically active individuals exhibited significant increases in SOD compared to inactive participants, and (3) prior meals of the participants; previous HFMs can cause an increased postprandial response.^8,41,43,44 The HFM used by Cardona et al is a commercial liquid preparation (Supracalw; SHS International, Liverpool, UK) containing 60 g of fat (12 g are saturated) while we used a typical HFM American breakfast consisting of a sausage and egg biscuit with hash browns (43 g fat and 16 g are saturated fat). In addition, Cardona et al did not provide information on physical activity or nutrient intake. These factors might explain the differences in SOD and GPx between the two studies.

Instead of antioxidant enzymes, other investigators have used total antioxidant capacity as a marker of oxidative stress response. Kay and Holub²¹ showed significant increases in oxygen radical absorbance capacity (ORAC) and total antioxidant status (TAS) after blueberry supplementation with an HFM. In a study investigating the response from vitamin C, strawberry, spinach, or red wine consumption in elderly females, Cao et al²² observed significant increases in ORAC and ferric-reducing ability of plasma (FRAP) after all supplements compared to a control. There was also a significant increase in trolox equivalent antioxidant capacity (TEAC) following the spinach supplement. Thirty-day mango supplementation in healthy adults increased antioxidant capacity which was proposed to be through absorption of antioxidant polyphenols or increasing endogenous antioxidant production.²⁵ In this study, looking at different markers of oxidative stress (malondialdehyde (MDA), oxidized LDL, and H₂O₂ and antioxidant status (ORAC, TAS, and TEAC), may have developed a more complete picture.

Strengths and experimental considerations

This study has several limitations. The first limitation is the absence of a true control group. We did not account for the extra kcal, carbohydrates, and other nutrients in the mango shake, as the mango supplement was simply added to the HFM. Moreover, the amount and specific types of carbohydrates that can influence the postprandial response (i.e. dietary fiber, monosaccharides, disaccharides, amylase, or amylopectin) found in the freeze-dried mango were also not accounted for. Nonetheless. Masibo and He²³ reported that the amount of insoluble, soluble, and total dietary fiber found in mango pulp are 13.80%, 14.25%, and 28.05% on dry matter basis, respectively. Similar concentrations of glucose, fructose, and sucrose are reported to be the major sugars in ripe mango.⁴⁵ Knowing the types of the carbohydrates in our freeze-dried mango and matching the control for the different carbohydrates, fiber, and caloric content may have provided a better picture of the effects of the mango fruit. However, the question of our study was to determine whether the addition of mango modulates the postprandial response to an HFM. In this context, our findings of a blunted glucose response and a greater HDL-C response are potentially meaningful, considering that the participants consumed additional carbohydrate and kcal in the mango trial and still exhibited improved outcomes.

Another major limitation of the study is the lack of assessment of insulin levels. This would have given us a more complete picture of the insulin response to the two meals and a more complete picture of the postprandial metabolic response. Next, our study sample was not very representative of the general population, since our cohort was comprised of only young males with generally healthy BMIs. Another limitation of the study is the markers of oxidative stress used. As previously mentioned, other markers of oxidation, such as H₂O₂, MDA, or oxidized LDL, would have provided information on whether or not mango reduced oxidative stress by free radical scavenging, instead of assessing antioxidant enzymes, which may require more time to see changes. Similarly, total antioxidant capacity was not evaluated, which would have provided a better picture of the overall response. As mentioned previously, some markers of inflammation, such as CRP, may take longer to change and would not be observed immediately within the 4 hours after a meal. Finally, the PAS 2 questionnaire provided the physical activity in a course of a single week and not whether the person exercise the day prior to the postprandial tests that can alter the postprandial response.

Despite the study limitations, the study did possess several strengths. To the best of our knowledge, this is the first study to look at postprandial clinical parameters, inflammation, and antioxidant enzymes, after acute mango consumption together with an HFM. The cross-over design allowed for participants to be their own control, reducing between-subject variability. The participants also consumed a traditional HFM providing a real-world application.

Conclusion

Acute mango consumption in conjunction with an HFM had modest effects on the postprandial response in young healthy males, including beneficial effects in glucose and HDL-C. However, there were no improvements in antioxidant enzymes or inflammation following the HFM supplemented with mango. Future studies should look at the effect of mango supplementation on the postprandial response in more at-risk populations, such as individuals who are older, over-weight, obese, diabetic, or have metabolic syndrome. The postprandial response has been shown to be exaggerated in these population^46-50 and may benefit more from dietary intervention. In addition, the effects of chronic mango supplementation on postprandial response should also be explored.

Footnotes

Acknowledgements

The authors would like to thank all the study participants, the staff at the Stillwater Medical Center, and the Oklahoma State University Department of Nutritional Sciences for support with this research.

Funding:

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the Oklahoma State University College of Human Sciences and through a grant from the National Mango Board. The National Mango Board had no involvement in the study design, data collection, data analysis, manuscript preparation, or decision in manuscript submission. Supported, in part, by a grant from the National Mango Board.

Declaration of conflicting interests:

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Author Contributions

Experimental design: EL, PP, MP, JH, and BS. Preparation of the freeze-dried mango powder: PP. Statistical analyses: MP. Research procedures: CO, BO, SE, AS, SP, PP, JH, BS, and EL. First draft of the manuscript: SE and CO. Revisions and final approved version of paper: SE, BS, and EL. All authors reviewed and approved of the final manuscript

ORCID iD

Edralin A Lucas

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