A peptide fraction (VUEFFE) obtained from bovine factor VIII, with a high affinity for vessel endothelium, was bound to fluorescein and used for angiography in rabbits. Several eyes had undergone laser photocoagulation on the retina. The location of the dye was studied histologically with fluorescence microscopy on the enucleated, freeze-dried eyes. The angiographic and histologic findings were compared with findings obtained using sodium fluorescein. Fluorescein-labelled VUEFFE gave a longer lasting fluorescence of the retinal vessels with histologic evidence of dye deposition on the vessel wall. It also showed marked affinity for the photocoagulated tissues, giving intense and prolonged staining of the laser burns. Possible clinical applications are discussed.
HochheimerB.F.Angiography of the retina with indocyanine green.Arch Ophthalmol1971; 86: 564–5.
6.
MachemerR.Angiographic - histologic correlation of eye vessel permeability with protein-bound fluorescent dye.Am J Ophthalmol1970; 69: 27–38.
7.
HochheimerB.F., D'AnnaS.A.Angiography with new dyes.Exp Eye Res1978; 27: 1–16.
8.
GrimesP.A., StoneR.A., LatiesA.M., LiW.Carboxyfluorescein. Probe of the blood-ocular barriers with lower membrane permeability than fluorescein.Arch Ophthalmol1982; 100: 635–9.
9.
OncelM., KhoobehiB., PeyhanG.Calcein angiography: a preliminary report on an experimental dye.International Ophthalmology1990; 14: 245–50.
10.
FangT., NaguibK.S., PeyhanG.A., KhoobehiB.Comparative study of three fluorescent dyes for angiography: sodium fluorescein, carboxyfluorescein and calcein.Ophthalmic Surgery1990; 21: 250–7.
11.
SollomA.W.Conjugated fluorescein for fundus photography.Br J Ophthalmol1968; 52: 691–4.
12.
McnaughtE.I., FouldsW.S., JohnsonN.F.The permeability of the posterior blood-ocular barrier after xenon photocoagulation: a study using fluorescein labelled dextrans.Br J Ophthalmol1981; 65: 473–7.
13.
WitteS.Surface phenomena of coagulation factors in vivo, observed by fluorescence intravital microscopy.Ann NY Acad Sci1983; 416: 426–40.
14.
GervasiG.B., BartoliC., CarpitaG., BaldacciM.Decrease of bleeding time by a peptide fraction from bovine Factor VIII in laboratory animals.Arzneimittel Forsch1988; 38: 1268–73.
15.
BaldacciM., CatalaniR., FascettiE., GervasiG.B.Polypeptide fraction from bovine Factor VIII does not influence human platelet aggregation and blood coagulation.Pharmacol Res Commun1988; 20: 1.
16.
AmmannatiP., SiravoD., GervasiG.B., GelsoE., BaldacciM.Polypeptide fraction from bovine Factor VIII used in fluorangiography.Ophthalmologica1988; 197: 51–5.
17.
GraysonH.E., LatiesA.M.Ocular localization of sodium fluorescein.Arch Ophthalmol1971; 85: 600–9.
18.
McMahonR.T., TsoM.O.M., McLeanI.W.Histologic localization of sodium fluorescein in human ocular tissues.Am J Ophthalmol1975; 80: 1058–65.
19.
RinderknechtH.Ultra-rapid fluorescent labelling of proteins.Nature1962; 193: 167–8.
20.
BartoliC., PellegriniN., BaldacciU.Research on toxicity and antigenic property of V.F. natural hemostatic. Internal Report: Research Department S.p.A. Pisa, 1978.
21.
BrubakerR.F., PennistoneJ.T., GrotteD.A., NagatakiS.Measurement of fluorescein binding in human plasma using fluorescence polarization.Arch Ophthalmol1982; 100: 625–30.
LanaconeD.C., FelbergN.T., FedermanJ.L.Tritiated fluorescein binding to normal human plasma proteins.Arch Ophthalmol1980; 98: 1643–5.
24.
LiW., RockeyJ.H.Fluorescein binding to normal human serum proteins demonstrated by equilibrium dialysis.Arch Ophthalmol1982; 100: 484–7.
25.
YannuzziL.A., SlakterJ.S., SorensonJ.A., GuyerD.R., OrlockD.A.Digital indocyanine green videoangiography and choroidal neovascularization.Retina (in press).
26.
SceiffarthO.F., ZrennerE., DiskoR., StefaniF.H., Brabandert. Intraocular in vivo immunofluorescence. A new technique for visualizing structures of the ocular fundus.Invest Ophthalmol Vis Sci1990; 31: 272–6.
