The present study compared the expression of brain-derived neurotrophic factor (BDNF) in the lateral geniculate body between form deprivation amblyopia kittens and normal kittens to examine the significance of BDNF in the lateral geniculate body in the pathogenesis of amblyopia.
Methods:
Twenty kittens were divided into control group (n = 10) and deprivation group (n = 10). A black opaque eye mask was placed to cover the right eye of the deprivation group. Pattern visual-evoked potentials (PVEPs) were detected weekly in all kittens .After the kittens in the deprivation group developed monocular amblyopia, the lateral geniculate bodies of all kittens were removed. The expression of BDNF in the lateral geniculate body of the two groups was compared by immunohistochemistry and Western blotting.
Results:
The latency of the P100 wave in the right eye of the deprivation group was longer than that of the left eye and that of the right eye of the control group (p < 0.05), and the amplitude decreased (p < 0.05). The number and average optical density of BDNF-positive cells in the deprivation group were lower than those in the control group (p < 0.05), and the expression of BDNF in the deprivation group was lower than that in the control group (p < 0.05).
Conclusions:
The expression of BDNF in the lateral geniculate body of the amblyopic kittens decreased, and the decrease in BDNF promoted the development of amblyopia. These results demonstrate that BDNF in the lateral geniculate body plays an important role in visual development.
BardeYAEdgarDThoenenH.Purification of a new neurotrophic factor from mammalian brain. EMBO J1982; 1(5): 549–553.
2.
ConnerJMLauterbornJCYanQ, et al. Distribution of brain-derived neurotrophic factor (BDNF) protein and mRNA in the normal adult rat CNS. Evidence for anterograde axonal transport. J Neurosci1997; 17(7): 2295–2313.
3.
YoshiiAConstantine-PatonM.Postsynaptic BDNF-TrkB signaling in synapse maturation, plasticity, and disease. Dev Neurobiol2010; 70(5): 304–322.
4.
MatsumotoTRauskolbSPolackM, et al. Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF. Nat Neurosci2008; 11(2): 131–133.
5.
DiStefanoPSFriedmanBRadziejewskiC, et al. The neurotrophins BDNF, NT-3, and NGF display distinct patterns of retrograde axonal transport in peripheral and central neurons. Neuron1992; 8(5): 983–993.
6.
KalishBTCheadleLHrvatinS, et al. Single-cell transcriptomics of the developing lateral geniculate nucleus reveals insights into circuit assembly and refinement. Proc Natl Acad Sci USA2018; 115(5): E1051–E1060.
7.
KraheTEEl-DanafRNDilgerEK, et al. Morphologically distinct classes of relay cells exhibit regional preferences in the dorsal lateral geniculate nucleus of the mouse. J Neurosci2011;31(48):17437–1748.
8.
ZhaoXChenHLiuX, et al. Orientation-selective responses in the mouse lateral geniculate nucleus. J Neurosci2013; 33(31): 12751–1263.
9.
RompaniSBMüllnerFEWannerA, et al. Different modes of visual integration in the lateral geniculate nucleus revealed by single-cell-initiated transsynaptic tracing. Neuron2017; 93(6): 1519.
10.
LeventhalAG.Evidence that the different classes of relay cells of the cat’s lateral geniculate nucleus terminate in different layers of the striate cortex. Exp Brain Res1979; 37(2): 349–372.
11.
PiscopoDMEl-DanafRNHubermanAD, et al. Diverse visual features encoded in mouse lateral geniculate nucleus. J Neurosci2013; 33(11): 4642–4656.
12.
KileyCWUsreyWM.Orientation tuning of correlated activity in the developing lateral geniculate nucleus. J Neurosci2017; 37(48): 11549–11558.
13.
WieselTNHubelDH.Effects of visual deprivation on morphology and physiology of cells in the cats lateral geniculate body. J Neurophysiol1963; 26: 978–93.
14.
BarnesGRLiXThompsonB, et al. Decreased gray matter concentration in the lateral geniculate nuclei in human amblyopes. Invest Ophthalmol Vis Sci2010; 51(3): 1432–1438.
15.
TropeaDCapsoniSTongiorgiE, et al. Mismatch between BDNF mRNA and protein expression in the developing visual cortex: the role of visual experience. Eur J Neurosci2001; 13(4): 709–721.
16.
MikiALiuGTGoldsmithZG, et al. Decreased activation of the lateral geniculate nucleus in a patient with anisometropic amblyopia demonstrated by functional magnetic resonance imaging. Ophthalmologica2003; 217(5): 365–369.
17.
KarpovaNNRantamäkiTDi LietoA, et al. Darkness reduces BDNF expression in the visual cortex and induces repressive chromatin remodeling at the BDNF gene in both hippocampus and visual cortex. Cell Mol Neurobiol2010; 30(7): 1117–1123.
18.
HubelDHWieselTN.The period of susceptibility to the physiological effects of unilateral eye closure in kittens. J Physiol1970; 206(2): 419–436.
19.
DuffyKRLingleyAJHolmanKD, et al. Susceptibility to monocular deprivation following immersion in darkness either late into or beyond the critical period. J Comp Neurol2016(13); 524: 2643–2653.
20.
ArdenGBBarnardWMMushinAS.Visually evoked responses in amblyopia. Br J Ophthalmol1974; 58(3): 183–192.
21.
SnyderAShapleyR.Deficits in the visual evoked potentials of cats as a result of visual deprivation. Exp Brain Res1979; 37(1): 73–86.
AhmedSReynoldsBAWeissS.BDNF enhances the differentiation but not the survival of CNS stem cell-derived neuronal precursors. J Neurosci1995; 15(8): 5765–5778.
24.
LindsayRM.Nerve growth factors (NGF, BDNF) enhance axonal regeneration but are not required for survival of adult sensory neurons. J Neurosci1988; 8(7): 2394–2405.
25.
HorchHWKatzLC.BDNF release from single cells elicits local dendritic growth in nearby neurons. Nat Neurosci2002; 5(11): 1177–1184.
26.
DesaiNSRutherfordLCTurrigianoGG.BDNF regulates the intrinsic excitability of cortical neurons. Learn Mem1999; 6(3): 284–291.
27.
MadedduFNaskaSMennaE, et al. Intraocular delivery of BDNF following visual cortex lesion upregulates cytosolic branched chain aminotransferase (BCATc) in the rat dorsal lateral geniculate nucleus. Eur J Neurosci2004; 20(2): 580–586.
28.
MadedduFNaskaSBozziY.BDNF down-regulates the caspase 3 pathway in injured geniculo-cortical neurones. Neuroreport2004; 15(13): 2045–2049.
29.
ChengBMattsonMP.NT-3 and BDNF protect CNS neurons against metabolic/excitotoxic insults. Brain Res1994; 640(1–2): 56–67.
30.
AlmeidaRDManadasBJMeloCV, et al. Neuroprotection by BDNF against glutamate-induced apoptotic cell death is mediated by ERK and PI3-kinase pathways. Cell Death Differ2005; 12(10): 1329–1343.
31.
DuffyKRFongMFMitchellDE, et al. Recovery from the anatomical effects of long-term monocular deprivation in cat lateral geniculate nucleus. J Comp Neurol2018; 526(2): 310–323.
32.
GaoMMaynardKRChokshiV, et al. Rebound potentiation of inhibition in juvenile visual cortex requires vision-induced BDNF expression. J Neurosci2014; 34(32): 10770–10779.
33.
JonesKRFariñasIBackusC, et al. Targeted disruption of the BDNF gene perturbs brain and sensory neuron development but not motor neuron development. Cell1994; 76(6): 989–999.
34.
AllendoerferKLCabelliRJEscandónE, et al. Regulation of neurotrophin receptors during the maturation of the mammalian visual system. J Neurosci1994; 14(3 Pt 2): 1795–1811.
35.
MandolesiGMennaEHarauzovA, et al. A role for retinal brain-derived neurotrophic factor in ocular dominance plasticity.Curr Biol2005; 15(23): 2119–2124.
36.
ReganD.Speedy assessment of visual acuity in amblyopia by the evoked potential method. Ophthalmologica1977; 175(3): 159–164.
37.
SuzukiMNagaeMNagataY, et al. Effects of refractive errors on visual evoked magnetic fields. BMC Ophthalmol2015; 15: 162.
38.
CastrénEZafraFThoenenH, et al. Light regulates expression of brain-derived neurotrophic factor mRNA in rat visual cortex. Proc Natl Acad Sci USA1992; 89(20): 9444–9448.
39.
DurandSIyerRMizusekiK, et al. A comparison of visual response properties in the lateral geniculate nucleus and primary visual cortex of awake and anesthetized mice. J Neurosci2016; 36(48): 12144–12156.
40.
GianfranceschiLSicilianoRWallsJ, et al. Visual cortex is rescued from the effects of dark rearing by overexpression of BDNF. Proc Natl Acad Sci USA2003; 100(21): 12486–12491.
41.
SanseveroGBaroncelliLScaliM, et al. Intranasal BDNF administration promotes visual function recovery in adult amblyopic rats. Neuropharmacology2019; 145(PtA): 114–122.
