Inhaling Odor-Active Compounds of Osmanthus fragrans Var. aurantiacus Suppresses Inflammation-Related Transcriptomic Signatures in LPS-Exposed SD Rat Brains: Applications of GC–MS–O and RNA Sequencing
Restricted accessResearch articleFirst published online June, 2026
Inhaling Odor-Active Compounds of Osmanthus fragrans Var. aurantiacus Suppresses Inflammation-Related Transcriptomic Signatures in LPS-Exposed SD Rat Brains: Applications of GC–MS–O and RNA Sequencing
This study investigated the effects of nasal inhalation of Osmanthus fragrans var. aurantiacus (OFA) extract in lipopolysaccharide (LPS)-challenged rats. Electronic nose analysis of the OFA extracts identified 25 volatile organic compounds. Gas chromatography–mass spectrometry–olfactometry (GC–MS–O) analysis revealed that β-ionone, linalool, and related derivatives are the primary odor-active compounds in the OFA extract. LPS exposure increased pro-inflammatory markers like tumor necrosis factor-alpha, caused organ hypertrophy in the liver, lungs, and spleen, and altered transcriptional profiles linked to nuclear factor-kappa B (NF-κΒ) and immune pathways. RNA sequencing of brain tissues showed that while LPS strongly elevated NF-κΒ and cytokine pathways, this effect was less pronounced in OFA-treated rats, indicating reduced LPS-driven inflammation at the transcriptome level. Overall, these findings highlight the potential of OFA extract, abundant in β-ionone and linalool, to mitigate inflammatory signaling and improve select metabolic markers in an LPS-induced inflammatory model.
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